Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cytoplasmic lattices store developmentally poised degradative and cytoskeletal complexes in mammalian eggs.
Journal, issue, pages	Nat Struct Mol Biol, Year 2026
Publish date	Jul 9, 2026
Authors	Yujie Li / Wei Zheng / Jiyeon Leem / Chunxiang Wu / Shaogeng Tang / Binyam Mogessie / Yong Xiong /
PubMed Abstract	The cytoplasmic lattice (CPL) in mammalian eggs is essential for early embryonic development but its molecular components, structural organization and functional capacity have remained elusive. Here, ...The cytoplasmic lattice (CPL) in mammalian eggs is essential for early embryonic development but its molecular components, structural organization and functional capacity have remained elusive. Here, using cryo-electron microscopy, we show that the CPL filament in mouse metaphase II eggs contains repeating units with a periodicity of ~37 nm and determine its high-resolution, native structure and complete subunit composition. The CPL architecture organizes maternal-effect proteins, ubiquitination machinery and tubulin into a highly structured reservoir. Maternal-effect proteins form the scaffold of the CPL to sequester a UHRF1-UBE2D3 E3-E2 ubiquitination module and three distinct FBXW-SKP1 E3 ubiquitin ligase components, notably all in activity-excluded states. The CPL further contains αβ-tubulin heterodimers in a GTP-bound state, indicating microtubule-assembly-competent tubulin held in reserve. CPL filaments are capped by a terminal unit that lacks a PADI6 dimer, a scaffold component, suggesting a structural mechanism that prevents further oligomerization. Interactions between neighboring CPL filaments promote the assembly of a three-dimensional network in the egg cytoplasm. Taken together, our work defines how CPL assembly and architecture prime mammalian eggs for ubiquitin-mediated protein degradation and cytoskeletal remodeling during the egg-to-embryo transition.
External links	Nat Struct Mol Biol / PubMed:42426369
Methods	EM (single particle)
Resolution	2.56 - 8.63 Å
Structure data	EMDB-76315: Native structure of cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on PADI6 dimers 1-4 Method: EM (single particle) / Resolution: 3.29 Å EMDB-76321: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3 Method: EM (single particle) / Resolution: 3.1 Å EMDB-76322: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+ZBED3+FBXW18 Method: EM (single particle) / Resolution: 3.03 Å EMDB-76323: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP14+UHRF1 Method: EM (single particle) / Resolution: 3.08 Å EMDB-76324: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on tubulin Method: EM (single particle) / Resolution: 3.09 Å EMDB-76325: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP4F Method: EM (single particle) / Resolution: 2.56 Å EMDB-76326: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on FBXW19+FBXW21 Method: EM (single particle) / Resolution: 3.29 Å EMDB-76327: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on UBE2D3+UHRF1 RING Method: EM (single particle) / Resolution: 3.49 Å EMDB-76330: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on PADI6 dimers 5 Method: EM (single particle) / Resolution: 3.51 Å EMDB-76331: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP14+UHRF1+UBE2D3 Method: EM (single particle) / Resolution: 4.16 Å EMDB-76333: Native structure of the cytoplasmic lattice filament from mouse MII eggs Method: EM (single particle) / Resolution: 8.63 Å 76334 12dl EMDB-76334, PDB-12dl: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs Method: EM (single particle) / Resolution: 3.5 Å EMDB-76335: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, consensus map Method: EM (single particle) / Resolution: 3.52 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-CA*: Unknown entry
Source	mus musculus (house mouse)
Keywords	CYTOSOLIC PROTEIN / cytoplasmic lattice / egg / filamentous assembly