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- EMDB-76321: Native structure of the cytoplasmic lattice (CPL) asymmetric unit... -

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Basic information

Entry
Database: EMDB / ID: EMD-76321
TitleNative structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3
Map data
Sample
  • Complex: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3
Keywordscytoplasmic lattice / egg / filamentous assembly / CYTOSOLIC PROTEIN
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsLi Y / Zheng W / Leem J / Wu C / Tang S / Mogessie B / Xiong Y
Funding support United States, 1 items
OrganizationGrant numberCountry
Other government United States
CitationJournal: Nat Struct Mol Biol / Year: 2026
Title: Cytoplasmic lattices store developmentally poised degradative and cytoskeletal complexes in mammalian eggs.
Authors: Yujie Li / Wei Zheng / Jiyeon Leem / Chunxiang Wu / Shaogeng Tang / Binyam Mogessie / Yong Xiong /
Abstract: The cytoplasmic lattice (CPL) in mammalian eggs is essential for early embryonic development but its molecular components, structural organization and functional capacity have remained elusive. Here, ...The cytoplasmic lattice (CPL) in mammalian eggs is essential for early embryonic development but its molecular components, structural organization and functional capacity have remained elusive. Here, using cryo-electron microscopy, we show that the CPL filament in mouse metaphase II eggs contains repeating units with a periodicity of ~37 nm and determine its high-resolution, native structure and complete subunit composition. The CPL architecture organizes maternal-effect proteins, ubiquitination machinery and tubulin into a highly structured reservoir. Maternal-effect proteins form the scaffold of the CPL to sequester a UHRF1-UBE2D3 E3-E2 ubiquitination module and three distinct FBXW-SKP1 E3 ubiquitin ligase components, notably all in activity-excluded states. The CPL further contains αβ-tubulin heterodimers in a GTP-bound state, indicating microtubule-assembly-competent tubulin held in reserve. CPL filaments are capped by a terminal unit that lacks a PADI6 dimer, a scaffold component, suggesting a structural mechanism that prevents further oligomerization. Interactions between neighboring CPL filaments promote the assembly of a three-dimensional network in the egg cytoplasm. Taken together, our work defines how CPL assembly and architecture prime mammalian eggs for ubiquitin-mediated protein degradation and cytoskeletal remodeling during the egg-to-embryo transition.
History
DepositionMar 27, 2026-
Header (metadata) releaseApr 8, 2026-
Map releaseApr 8, 2026-
UpdateJul 29, 2026-
Current statusJul 29, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_76321.png

Downloads & links

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Map

FileDownload / File: emd_76321.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

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AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 512 pix.
= 546.816 Å		1.07 Å/pix.
x 512 pix.
= 546.816 Å		1.07 Å/pix.
x 512 pix.
= 546.816 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.068 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.5116864 - 1.1758604
Average (Standard dev.)0.002328175 (±0.025168853)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 546.816 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_76321_msk_1.map
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Half map: #1

Fileemd_76321_half_map_1.map
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Half map: #2

Fileemd_76321_half_map_2.map
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Sample components

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Entire : Native structure of the cytoplasmic lattice (CPL) asymmetric unit...

EntireName: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3
Components
  • Complex: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3

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Supramolecule #1: Native structure of the cytoplasmic lattice (CPL) asymmetric unit...

SupramoleculeName: Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs, focused on NLRP5+TLE6+OOEP+KHDC3
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Mus musculus (house mouse)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 249541
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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