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TitleStructure of an Ancient Respiratory System.
Journal, issue, pagesCell, Vol. 173, Issue 7, Page 1636-1649.e16, Year 2018
Publish dateJun 14, 2018
AuthorsHongjun Yu / Chang-Hao Wu / Gerrit J Schut / Dominik K Haja / Gongpu Zhao / John W Peters / Michael W W Adams / Huilin Li /
PubMed AbstractHydrogen gas-evolving membrane-bound hydrogenase (MBH) and quinone-reducing complex I are homologous respiratory complexes with a common ancestor, but a structural basis for their evolutionary ...Hydrogen gas-evolving membrane-bound hydrogenase (MBH) and quinone-reducing complex I are homologous respiratory complexes with a common ancestor, but a structural basis for their evolutionary relationship is lacking. Here, we report the cryo-EM structure of a 14-subunit MBH from the hyperthermophile Pyrococcus furiosus. MBH contains a membrane-anchored hydrogenase module that is highly similar structurally to the quinone-binding Q-module of complex I while its membrane-embedded ion-translocation module can be divided into a H- and a Na-translocating unit. The H-translocating unit is rotated 180° in-membrane with respect to its counterpart in complex I, leading to distinctive architectures for the two respiratory systems despite their largely conserved proton-pumping mechanisms. The Na-translocating unit, absent in complex I, resembles that found in the Mrp H/Na antiporter and enables hydrogen gas evolution by MBH to establish a Na gradient for ATP synthesis near 100°C. MBH also provides insights into Mrp structure and evolution of MBH-based respiratory enzymes.
External linksCell / PubMed:29754813 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

7468

6cfw

EMDB-7468, PDB-6cfw:
cryoEM structure of a respiratory membrane-bound hydrogenase
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-NFU:
formyl[bis(hydrocyanato-1kappaC)]ironnickel(Fe-Ni)

Source
  • Pyrococcus furiosus (archaea)
  • pyrococcus furiosus com1 (archaea)
  • pyrococcus furiosus (strain atcc 43587 / dsm 3638 / jcm 8422 / vc1) (archaea)
KeywordsMEMBRANE PROTEIN / respiratory / hydrogenase / ion translocation

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