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TitleCryo-EM structure of the gasdermin A3 membrane pore.
Journal, issue, pagesNature, Vol. 557, Issue 7703, Page 62-67, Year 2018
Publish dateApr 25, 2018
AuthorsJianbin Ruan / Shiyu Xia / Xing Liu / Judy Lieberman / Hao Wu /
PubMed AbstractGasdermins mediate inflammatory cell death after cleavage by caspases or other, unknown enzymes. The cleaved N-terminal fragments bind to acidic membrane lipids to form pores, but the mechanism of ...Gasdermins mediate inflammatory cell death after cleavage by caspases or other, unknown enzymes. The cleaved N-terminal fragments bind to acidic membrane lipids to form pores, but the mechanism of pore formation remains unresolved. Here we present the cryo-electron microscopy structures of the 27-fold and 28-fold single-ring pores formed by the N-terminal fragment of mouse GSDMA3 (GSDMA3-NT) at 3.8 and 4.2 Å resolutions, and of a double-ring pore at 4.6 Å resolution. In the 27-fold pore, a 108-stranded anti-parallel β-barrel is formed by two β-hairpins from each subunit capped by a globular domain. We identify a positively charged helix that interacts with the acidic lipid cardiolipin. GSDMA3-NT undergoes radical conformational changes upon membrane insertion to form long, membrane-spanning β-strands. We also observe an unexpected additional symmetric ring of GSDMA3-NT subunits that does not insert into the membrane in the double-ring pore, which may represent a pre-pore state of GSDMA3-NT. These structures provide a basis that explains the activities of several mutant gasdermins, including defective mutants that are associated with cancer.
External linksNature / PubMed:29695864 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 4.6 Å
Structure data

7449

6cb8

EMDB-7449, PDB-6cb8:
Cryo-EM structure of the Gasdermin A3 membrane pore
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-7450:
Cryo-EM structure of the Gasdermin A3 membrane pore
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-7451:
Cryo-EM structure of the Gasdermin A3 membrane pore
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Source
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / Pyroptosis / Pore forming protein

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