EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational landscape and ligand-dependent clustering of the human type 2 IP receptor.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年6月19日
著者	Caifeng Liu / Yu-Jing Lan / Max G Kushner / Qingyu Tang / Erkan Karakas /
PubMed 要旨	Inositol 1,4,5-trisphosphate (IP) receptors (IPRs) are tetrameric ER Ca channels that shape intracellular Ca signaling in response to IP, regulating diverse physiological processes. The structural ...Inositol 1,4,5-trisphosphate (IP) receptors (IPRs) are tetrameric ER Ca channels that shape intracellular Ca signaling in response to IP, regulating diverse physiological processes. The structural basis for subtype-specific regulation among the three subtypes (IPR-1-3) remains incompletely understood due to the lack of IPR-2 structures. Here, we report cryo-electron microscopy (cryo-EM) structures of human IPR-2 in distinct conformations in the presence and absence of IP, Ca, and ATP. These structures define the conformational landscape of IPR-2, delineate ligand-binding interactions, and reveal shared architectural features alongside isoform-specific differences. We also resolve ligand-dependent IPR-2 assemblies, identifying a conformation-dependent inter-channel interface. Live-cell imaging demonstrates that IPR-2 undergoes clustering following ligand-induced Ca release, and disruption of this interface selectively abolishes clustering without impairing channel activity. Together, these findings provide a structural framework for human IPR-2 and establish a mechanism linking ligand-dependent conformational changes to inter-channel interactions and post-activation cellular clustering.
リンク	Nat Commun / PubMed:42315508 / PubMed Central
手法	EM (単粒子)
解像度	2.71 - 4.47 Å
構造データ	EMDB-73048: Human type 2 IP3 receptor apo state consensus map 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-73049: Human type 2 IP3 receptor apo state ligand binding domain (LBD) local refinement 手法: EM (単粒子) / 解像度: 2.72 Å EMDB-73050: Human type 2 IP3 receptor apo state central linker domain (CLD) local refinement 手法: EM (単粒子) / 解像度: 2.88 Å EMDB-73051: Human type 2 IP3 receptor apo state ARM2 domain local refinement 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-73052: Human type 2 IP3 receptor apo state ARM3-JD domain local refinement 手法: EM (単粒子) / 解像度: 2.71 Å EMDB-73053: Human type 2 IP3 receptor apo state transmembrane domain (TMD) local refinement 手法: EM (単粒子) / 解像度: 2.78 Å 73054 9ykk EMDB-73054, PDB-9ykk: Human type 2 IP3 receptor in apo state 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-73061: IP3/ATP bound human type 2 IP3 receptor in the resting state - Consensus map 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-73062: Local refined ligand-binding domain (LBD) of IP3/ATP bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-73063: Local refined central linker domain (CLD) of IP3/ATP bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-73064: Local refined ARM2 domain of IP3/ATP bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-73065: Local refined ARM3-JD domains of IP3/ATP bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-73066: Local refined transmembrane domain (TMD) of IP3/ATP bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.23 Å 73067 9yky EMDB-73067, PDB-9yky: IP3/ATP-bound human type 2 IP3 receptor in the resting state 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-73082: IP3/ATP-bound human type 2 IP3 receptor in the preactivated state - Consensus map 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-73083: Local refined ligand-binding domain (LBD) of IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-73084: Local refined central linker domain (CLD) of IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 3.24 Å EMDB-73085: Local refined ARM2 domain of IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 3.24 Å EMDB-73086: Local refined ARM3-JD domains of IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-73087: Local refined transmembrane domain (TMD) of IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 2.91 Å 73088 9yli EMDB-73088, PDB-9yli: IP3/ATP-bound human type 2 IP3 receptor in the preactivated state 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-73111: Human type 2 IP3 receptor in the inactive-like state (IP3/ATP) Consensus Map 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-73112: Local refined ligand-binding domain (LBD, chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-73113: Local refined central linker domain (CLD, chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.08 Å EMDB-73114: Local refined ARM2 (chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-73115: Local refined ARM3-JD (chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 2.86 Å EMDB-73116: Local refined central linker domain (CLD, chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-73117: Local refined ARM2 (chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.47 Å EMDB-73118: Local refined ARM3-JD (chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 2.92 Å EMDB-73119: Local refined ARM3-JD (chain C) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.01 Å EMDB-73120: Local refined JD (chain D) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-73121: Local refined transmembrane domain (TMD) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 2.88 Å 73129 9ymz EMDB-73129, PDB-9ymz: Human type 2 IP3 receptor in the inactive-like state (IP3/ATP) 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-73198: Human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) Consensus Map 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-73199: Local refined ligand-binding domain (LBD, chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.89 Å EMDB-73201: Local refined central linker domain (CLD, chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-73202: Local refined ARM2 (chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.75 Å EMDB-73203: Local refined ARM3-JD (chain A) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-73204: Local refined central linker domain (CLD, chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.66 Å EMDB-73205: Local refined ARM2 (chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.98 Å EMDB-73206: Local refined ARM3-JD (chain B) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-73207: Local refined ARM3-JD (chain C) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.68 Å EMDB-73208: Local refined JD (chain D) of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-73209: Local refined JD and TMD of human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.15 Å 73210 9ynk EMDB-73210, PDB-9ynk: Human type 2 IP3 receptor in the inactive-like state (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-73212: Human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) Consensus Map 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-73213: Local refined dimerization interface of human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-73214: Local refined central linker domain (CLD, chain A) of human type 2 IP3 receptor in dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.85 Å EMDB-73215: Local refined ARM2 (chain A) of human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-73216: Local refined ARM3-JD (chain A) of human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-73217: Local refined N-terminal domain (NTD, chain B) of human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 4.23 Å EMDB-73218: Local refined JD and TMD of human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 4.06 Å 73219 9yno EMDB-73219, PDB-9yno: Human type 2 IP3 receptor dimer (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-73222: Human type 2 IP3 receptor dimer complete map (IP3/ATP/Ca2+) 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-73223: Human type 2 IP3 receptor in the intermediate state 3 (IP3/ATP) 手法: EM (単粒子) / 解像度: 4.47 Å EMDB-73224: Human type 2 IP3 receptor in the intermediate state 4 (IP3/ATP) 手法: EM (単粒子) / 解像度: 4.17 Å EMDB-73225: Human type 2 IP3 receptor in the inactive-like state (ligand-free) 手法: EM (単粒子) / 解像度: 3.99 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-I3P: D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-CA*: Unknown entry
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / Inositol 1 / 4 / 5-triphosphate / IP3 / receptor / calcium channel / type-2 / IP3R / IP3R-2 / ITPR2