EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanisms of disassembly at the actin filament pointed and barbed ends.
ジャーナル・号・ページ	Sci Adv, Vol. 12, Issue 14, Page eaee5882, Year 2026
掲載日	2026年4月3日
著者	Nicholas J Palmer / Malgorzata Boczkowska / Grzegorz Rebowski / Roberto Dominguez /
PubMed 要旨	Actin cytoskeleton dynamics power processes from cell motility to organelle trafficking, requiring rapid polymerization and depolymerization accelerated in cells by regulatory proteins. While ...Actin cytoskeleton dynamics power processes from cell motility to organelle trafficking, requiring rapid polymerization and depolymerization accelerated in cells by regulatory proteins. While mechanisms of accelerated polymerization are relatively well studied, those of depolymerization remain poorly understood. Here, we present twelve cryo-electron microscopy structures showing how cofilin, cyclase-associated protein (CAP), and capping protein (CP) coordinate their activities to accelerate depolymerization at both filament ends. Alone, CAP produces a ~4.0 Å lateral displacement of the first pointed-end subunit, whereas cofilin reverts terminal subunits at the pointed and barbed ends to a G-actin-like conformation and undertwists the filament short-pitch helix. When functioning together, these cofilin- and CAP-induced conformational changes are amplified to accelerate pointed-end disassembly. At the barbed end, the cofilin-induced changes trigger stepwise CP dissociation and favor depolymerization. These findings support end-specific mechanisms of filament disassembly through accelerated subunit dissociation, slowed subunit addition, and barbed-end uncapping.
リンク	Sci Adv / PubMed:41931606 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	2.06 - 4.21 Å
構造データ	EMDB-72302: Pointed end of Cofilin-2 Bound F-actin, Consensus Map 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-72303: Pointed end of Cofilin-2 Bound F-actin, Focused Refinement on Terminal Pointed End Actins 手法: EM (単粒子) / 解像度: 3.23 Å EMDB-72304: Pointed end of Cofilin-2 Bound F-actin, Focused Refinement on Actin Filament 手法: EM (単粒子) / 解像度: 3.09 Å 72305 9q7k EMDB-72305, PDB-9q7k: Pointed end of Cofilin-2 Bound F-actin 手法: EM (単粒子) / 解像度: 3.13 Å 72306 9q7l EMDB-72306: Barbed End of Cofilin-2 F-actin, B0 and B-1 Unoccupied PDB-9q7l: Barbed end of F-actin and cofilin on sides 手法: EM (単粒子) / 解像度: 3.68 Å 72307 9q7m EMDB-72307: Barbed End of Cofilin-2 F-actin, B-1 Occupied PDB-9q7m: Barbed end of cofilin actin, cofilin on second-to-last barbed end subunit 手法: EM (単粒子) / 解像度: 3.5 Å 72308 9q7n EMDB-72308: Barbed End of Cofilin-2 F-actin, Terminal Actins Occupied with Cofilin PDB-9q7n: Cofilin barbed end, cofilin on the two barbed end subunits 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-72310: One CAP-1 Bound to the Pointed End of F-actin, Consensus Map 手法: EM (単粒子) / 解像度: 3.02 Å EMDB-72311: One CAP-1 Bound to the Pointed End of F-actin, HFD Local Refinement 手法: EM (単粒子) / 解像度: 3.51 Å EMDB-72312: One CAP-1 Bound to the Pointed End of F-actin, Actin Local Refinement 手法: EM (単粒子) / 解像度: 2.9 Å 72313 9q7o EMDB-72313, PDB-9q7o: One CAP-1 Bound to the Pointed End of F-actin 手法: EM (単粒子) / 解像度: 3.02 Å EMDB-72326: Two CAP-1 Bound to the Pointed End of F-actin, Consensus Map 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-72327: Two CAP-1 Bound to the Pointed End of F-actin, CAP1 HFD Local Refinement 手法: EM (単粒子) / 解像度: 3.96 Å EMDB-72329: Two CAP-1 Bound to the Pointed End of F-actin, Actin Local Refinement 手法: EM (単粒子) / 解像度: 3.21 Å 72330 9xye EMDB-72330, PDB-9xye: Two CAP-1 Bound to the Pointed End of F-actin 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-72498: One CAP-1 Bound to the Pointed End of Cofilin F-actin, Consensus map 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-72504: One CAP-1 Bound to the Pointed End of Cofilin F-actin, PE local map 手法: EM (単粒子) / 解像度: 3.61 Å EMDB-72505: One CAP-1 Bound to the Pointed End of Cofilin F-actin, Actin local refinement 手法: EM (単粒子) / 解像度: 3.33 Å 72506 9y52 EMDB-72506, PDB-9y52: One CAP-1 Bound to the Pointed End of Cofilin F-actin 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-72700: Two CAP-1 bound to the pointed end of cofilin F-actin, actin local refinement 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-72701: Two CAP-1 bound to the pointed end of cofilin F-actin, pointed end local refinement 手法: EM (単粒子) / 解像度: 4.21 Å EMDB-72702: Two CAP-1 bound to the pointed end of cofilin F-actin 手法: EM (単粒子) / 解像度: 3.58 Å 72703 9y9j EMDB-72703, PDB-9y9j: Two CAP-1 Bound to the Pointed End of Cofilin F-actin 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-72704: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, actin local refinement 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-72705: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, capping protein local refinement 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-72706: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, consensus map 手法: EM (単粒子) / 解像度: 3.06 Å 72707 9y9l EMDB-72707, PDB-9y9l: CP at the barbed end with one cofilin on second-to-last subunit 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-72708: Capping protein bound to the barbed end of cofilactin, actin local refinement 手法: EM (単粒子) / 解像度: 2.98 Å EMDB-72709: Capping protein bound to the barbed end of cofilactin, barbed end local refinement 手法: EM (単粒子) / 解像度: 3.09 Å EMDB-72710: Capping protein bound to the barbed end of cofilactin, consensus refinement 手法: EM (単粒子) / 解像度: 3.06 Å 72711 9y9m EMDB-72711, PDB-9y9m: Capping protein bound to the barbed end of cofilactin 手法: EM (単粒子) / 解像度: 3.06 Å 72712 9y9p EMDB-72712, PDB-9y9p: Cofilactin filament 手法: EM (らせん対称) / 解像度: 2.06 Å EMDB-72996: Capping protein bound to the barbed end of F-actin, Barbed end local refinement 手法: EM (単粒子) / 解像度: 2.61 Å EMDB-72997: Capping protein bound to the barbed end of F-actin, Consensus refinement 手法: EM (単粒子) / 解像度: 2.62 Å 72998 9yim EMDB-72998, PDB-9yim: Capping protein bound to the barbed end of F-actin 手法: EM (単粒子) / 解像度: 2.62 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-HOH*: WATER
由来	homo sapiens (ヒト) oryctolagus cuniculus (ウサギ)
キーワード	PROTEIN FIBRIL / Cytoskeleton / Actin / Filament / Helical