Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mechanisms of disassembly at the actin filament pointed and barbed ends.
Journal, issue, pages	Sci Adv, Vol. 12, Issue 14, Page eaee5882, Year 2026
Publish date	Apr 3, 2026
Authors	Nicholas J Palmer / Malgorzata Boczkowska / Grzegorz Rebowski / Roberto Dominguez /
PubMed Abstract	Actin cytoskeleton dynamics power processes from cell motility to organelle trafficking, requiring rapid polymerization and depolymerization accelerated in cells by regulatory proteins. While ...Actin cytoskeleton dynamics power processes from cell motility to organelle trafficking, requiring rapid polymerization and depolymerization accelerated in cells by regulatory proteins. While mechanisms of accelerated polymerization are relatively well studied, those of depolymerization remain poorly understood. Here, we present twelve cryo-electron microscopy structures showing how cofilin, cyclase-associated protein (CAP), and capping protein (CP) coordinate their activities to accelerate depolymerization at both filament ends. Alone, CAP produces a ~4.0 Å lateral displacement of the first pointed-end subunit, whereas cofilin reverts terminal subunits at the pointed and barbed ends to a G-actin-like conformation and undertwists the filament short-pitch helix. When functioning together, these cofilin- and CAP-induced conformational changes are amplified to accelerate pointed-end disassembly. At the barbed end, the cofilin-induced changes trigger stepwise CP dissociation and favor depolymerization. These findings support end-specific mechanisms of filament disassembly through accelerated subunit dissociation, slowed subunit addition, and barbed-end uncapping.
External links	Sci Adv / PubMed:41931606 / PubMed Central
Methods	EM (single particle) / EM (helical sym.)
Resolution	2.06 - 4.21 Å
Structure data	EMDB-72302: Pointed end of Cofilin-2 Bound F-actin, Consensus Map Method: EM (single particle) / Resolution: 3.13 Å EMDB-72303: Pointed end of Cofilin-2 Bound F-actin, Focused Refinement on Terminal Pointed End Actins Method: EM (single particle) / Resolution: 3.23 Å EMDB-72304: Pointed end of Cofilin-2 Bound F-actin, Focused Refinement on Actin Filament Method: EM (single particle) / Resolution: 3.09 Å 72305 9q7k EMDB-72305, PDB-9q7k: Pointed end of Cofilin-2 Bound F-actin Method: EM (single particle) / Resolution: 3.13 Å 72306 9q7l EMDB-72306: Barbed End of Cofilin-2 F-actin, B0 and B-1 Unoccupied PDB-9q7l: Barbed end of F-actin and cofilin on sides Method: EM (single particle) / Resolution: 3.68 Å 72307 9q7m EMDB-72307: Barbed End of Cofilin-2 F-actin, B-1 Occupied PDB-9q7m: Barbed end of cofilin actin, cofilin on second-to-last barbed end subunit Method: EM (single particle) / Resolution: 3.5 Å 72308 9q7n EMDB-72308: Barbed End of Cofilin-2 F-actin, Terminal Actins Occupied with Cofilin PDB-9q7n: Cofilin barbed end, cofilin on the two barbed end subunits Method: EM (single particle) / Resolution: 3.48 Å EMDB-72310: One CAP-1 Bound to the Pointed End of F-actin, Consensus Map Method: EM (single particle) / Resolution: 3.02 Å EMDB-72311: One CAP-1 Bound to the Pointed End of F-actin, HFD Local Refinement Method: EM (single particle) / Resolution: 3.51 Å EMDB-72312: One CAP-1 Bound to the Pointed End of F-actin, Actin Local Refinement Method: EM (single particle) / Resolution: 2.9 Å 72313 9q7o EMDB-72313, PDB-9q7o: One CAP-1 Bound to the Pointed End of F-actin Method: EM (single particle) / Resolution: 3.02 Å EMDB-72326: Two CAP-1 Bound to the Pointed End of F-actin, Consensus Map Method: EM (single particle) / Resolution: 3.18 Å EMDB-72327: Two CAP-1 Bound to the Pointed End of F-actin, CAP1 HFD Local Refinement Method: EM (single particle) / Resolution: 3.96 Å EMDB-72329: Two CAP-1 Bound to the Pointed End of F-actin, Actin Local Refinement Method: EM (single particle) / Resolution: 3.21 Å 72330 9xye EMDB-72330, PDB-9xye: Two CAP-1 Bound to the Pointed End of F-actin Method: EM (single particle) / Resolution: 3.18 Å EMDB-72498: One CAP-1 Bound to the Pointed End of Cofilin F-actin, Consensus map Method: EM (single particle) / Resolution: 3.46 Å EMDB-72504: One CAP-1 Bound to the Pointed End of Cofilin F-actin, PE local map Method: EM (single particle) / Resolution: 3.61 Å EMDB-72505: One CAP-1 Bound to the Pointed End of Cofilin F-actin, Actin local refinement Method: EM (single particle) / Resolution: 3.33 Å 72506 9y52 EMDB-72506, PDB-9y52: One CAP-1 Bound to the Pointed End of Cofilin F-actin Method: EM (single particle) / Resolution: 3.46 Å EMDB-72700: Two CAP-1 bound to the pointed end of cofilin F-actin, actin local refinement Method: EM (single particle) / Resolution: 3.45 Å EMDB-72701: Two CAP-1 bound to the pointed end of cofilin F-actin, pointed end local refinement Method: EM (single particle) / Resolution: 4.21 Å EMDB-72702: Two CAP-1 bound to the pointed end of cofilin F-actin Method: EM (single particle) / Resolution: 3.58 Å 72703 9y9j EMDB-72703, PDB-9y9j: Two CAP-1 Bound to the Pointed End of Cofilin F-actin Method: EM (single particle) / Resolution: 3.58 Å EMDB-72704: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, actin local refinement Method: EM (single particle) / Resolution: 2.99 Å EMDB-72705: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, capping protein local refinement Method: EM (single particle) / Resolution: 3.16 Å EMDB-72706: Capping protein bound to the barbed end of cofilactin, co-bound with cofilin on the B-1 actin, consensus map Method: EM (single particle) / Resolution: 3.06 Å 72707 9y9l EMDB-72707, PDB-9y9l: CP at the barbed end with one cofilin on second-to-last subunit Method: EM (single particle) / Resolution: 3.06 Å EMDB-72708: Capping protein bound to the barbed end of cofilactin, actin local refinement Method: EM (single particle) / Resolution: 2.98 Å EMDB-72709: Capping protein bound to the barbed end of cofilactin, barbed end local refinement Method: EM (single particle) / Resolution: 3.09 Å EMDB-72710: Capping protein bound to the barbed end of cofilactin, consensus refinement Method: EM (single particle) / Resolution: 3.06 Å 72711 9y9m EMDB-72711, PDB-9y9m: Capping protein bound to the barbed end of cofilactin Method: EM (single particle) / Resolution: 3.06 Å 72712 9y9p EMDB-72712, PDB-9y9p: Cofilactin filament Method: EM (helical sym.) / Resolution: 2.06 Å EMDB-72996: Capping protein bound to the barbed end of F-actin, Barbed end local refinement Method: EM (single particle) / Resolution: 2.61 Å EMDB-72997: Capping protein bound to the barbed end of F-actin, Consensus refinement Method: EM (single particle) / Resolution: 2.62 Å 72998 9yim EMDB-72998, PDB-9yim: Capping protein bound to the barbed end of F-actin Method: EM (single particle) / Resolution: 2.62 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-HOH*: WATER
Source	homo sapiens (human) oryctolagus cuniculus (rabbit)
Keywords	PROTEIN FIBRIL / Cytoskeleton / Actin / Filament / Helical