EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structure of a thermostable phage's portal vertex and neck complex illuminates the headful maturation mechanism.
ジャーナル・号・ページ	J Mol Biol, Page 169641, Year 2026
掲載日	2026年1月14日
著者	Emma L Sedivy / Emily Agnello / Julia E Hobaugh / Rakeyah Ahsan / Kangkang Song / Chen Xu / Brian A Kelch /
PubMed 要旨	Viruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged ...Viruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged into its capsid shell through the portal complex. The portal complex then closes to retain DNA and connects to the tail, which is required for host recognition and infection. The trigger to stop pumping DNA and assemble the mature virus has been a longstanding conundrum in the field. We determined the structure of the portal, the proteins that connect it to the tail, and portal vertex in the hyperthermophilic phage Oshimavirus using cryo-Electron Microscopy (cryo-EM). We find highly intertwined loop structures, like in a wicker basket, potentially stabilizing the portal vertex against high temperatures. Moreover, we observe that the portal protrudes from the capsid in mature virions. We propose that portal is repositioned by packaged DNA, forming a pressure-sensitive switch that terminates genome packaging and triggers tail attachment in headful phages.
リンク	J Mol Biol / PubMed:41544933
手法	EM (単粒子)
解像度	2.3 - 7.3 Å
構造データ	70278 9oab EMDB-70278, PDB-9oab: C3 reconstruction of the thermophilic bacteriophage P74-26 Neck 手法: EM (単粒子) / 解像度: 2.6 Å 70279 9oac EMDB-70279, PDB-9oac: C5 reconstruction of the thermophilic bacteriophage P74-26 Portal Vertex 手法: EM (単粒子) / 解像度: 2.6 Å 70280 9oad EMDB-70280, PDB-9oad: C1 reconstruction of the thermophilic bacteriophage P74-26 Portal and Portal Vertex 手法: EM (単粒子) / 解像度: 3.56 Å 70281 9oae EMDB-70281, PDB-9oae: Composite reconstruction of the thermophilic bacteriophage P74-26 Neck and Portal Vertex 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-70282: C3 reconstruction of the thermophilic bacteriophage P74-26 Capsid-less Neck 手法: EM (単粒子) / 解像度: 2.74 Å EMDB-70283: C12 reconstruction of the thermophilic bacteriophage P74-26 Neck 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-70284: C12 reconstruction of the thermophilic bacteriophage P74-26 Collar-less Neck 手法: EM (単粒子) / 解像度: 2.6 Å 72288 9q7a EMDB-72288, PDB-9q7a: dsDNA in the central channel of the bacteriophage P74-26 neck 手法: EM (単粒子) / 解像度: 4.54 Å EMDB-72289: Asymmetric reconstruction of filled phage capsids lacking neck and tail 手法: EM (単粒子) / 解像度: 7.3 Å EMDB-72290: Asymmetric reconstruction of filled phage capsids with broken tails 手法: EM (単粒子) / 解像度: 5.8 Å
由来	oshimavirus p7426 (ウイルス)
キーワード	VIRUS / bacteriophage / thermophilic / Neck / Portal / Capsid / VIRUS/DNA / VIRUS-DNA complex