Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Yeast Rad55-Rad57-SHU paralog complex dynamically promotes Rad51 filament formation.
Journal, issue, pages	Mol Cell, Year 2026
Publish date	Jul 21, 2026
Authors	Christopher W Koo / Steven K Gore / Soo Y Ro / Jie Liu / Christine Yu / Caleigh M Azumaya / Bobby Brillantes / Inna Zilberleyb / Henry Chen / Mariam B Rafiqzada / Lyra Garcia Sanchez / Wolf-Dietrich Heyer / Claudio Ciferri / Stanislau Yatskevich /
PubMed Abstract	Homologous recombination (HR) is an important DNA repair pathway that safeguards genome integrity. During HR, the Rad51 nucleoprotein filaments catalyze strand invasion into a homologous duplex DNA. ...Homologous recombination (HR) is an important DNA repair pathway that safeguards genome integrity. During HR, the Rad51 nucleoprotein filaments catalyze strand invasion into a homologous duplex DNA. Filament formation requires a conserved family of Rad51 paralogs that act as tumor suppressors in humans. By capturing six distinct states using cryo-electron microscopy, we reveal that the Saccharomyces cerevisiae Rad51 paralog complex, composed of the Rad55-Rad57 heterodimer and the SHU (Psy3-Csm2-Shu1-Shu2) complex, selectively brings Rad51 to single-stranded DNA to seed filament formation. Rad51 itself is a transient yet integral component of this machinery which binds along the Rad57 subunit to complete a high-affinity DNA-binding site. We also uncover a dual-nucleotide regulatory mechanism: a structural ADP molecule stabilizes the complex, while a second, catalytic ATPase site at the Rad57-Rad51 interface promotes the release of the paralog complex. These structural and mechanistic features provide a blueprint for understanding the function of Rad51 paralogs across eukaryotes.
External links	Mol Cell / PubMed:42480524
Methods	EM (single particle)
Resolution	2.65 - 5.75 Å
Structure data	72156 9q2c EMDB-72156: RAD55C-Rad51 bound to ssDNA with AMP-PNP PDB-9q2c: Rad55-Rad57-SHU-Rad51 bound to ssDNA with AMP-PNP Method: EM (single particle) / Resolution: 3.06 Å 72162 9q2e EMDB-72162, PDB-9q2e: Rad55-Rad57-SHU bound to ssDNA Method: EM (single particle) / Resolution: 3.44 Å 72163 9q2f EMDB-72163, PDB-9q2f: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP Method: EM (single particle) / Resolution: 3.06 Å 72165 9q2h EMDB-72165, PDB-9q2h: Rad55-Rad57-SHU homologous recombination complex Method: EM (single particle) / Resolution: 2.74 Å 72166 9q2i EMDB-72166, PDB-9q2i: Rad55-Rad57-SHU homologous recombination complex Method: EM (single particle) / Resolution: 3.0 Å 72167 9q2l EMDB-72167, PDB-9q2l: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP Method: EM (single particle) / Resolution: 3.7 Å EMDB-72226: Rad55-Rad57-SHU bound to ssDNA with AMP-PNP. Local map focused on 55/57 Method: EM (single particle) / Resolution: 3.75 Å EMDB-72228: Rad55-Rad57-SHU Method: EM (single particle) / Resolution: 3.73 Å EMDB-72229: Rad55-Rad57-SHU - Composite map Method: EM (single particle) / Resolution: 3.56 Å EMDB-72237: Rad55-Rad57-SHU-Rad51 Method: EM (single particle) / Resolution: 2.97 Å EMDB-72241: "Rad55-Rad57-SHU-Rad51 - Composite map Method: EM (single particle) / Resolution: 3.06 Å EMDB-72245: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on Rad55/Rad57 Method: EM (single particle) / Resolution: 3.07 Å EMDB-72246: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on SHU Method: EM (single particle) / Resolution: 3.49 Å EMDB-72247: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Local map focused on Rad51 Method: EM (single particle) / Resolution: 3.37 Å EMDB-72249: Rad55-Rad57-SHU-Rad51-Rad51 bound to ssDNA with AMP-PNP. Composite map Method: EM (single particle) / Resolution: 3.06 Å EMDB-72252: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on Rad55/Rad57 Method: EM (single particle) / Resolution: 3.37 Å EMDB-72253: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on SHU Method: EM (single particle) / Resolution: 3.74 Å EMDB-72254: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Local map focused on Rad51 Method: EM (single particle) / Resolution: 3.4 Å EMDB-72259: Rad55-Rad57(E161Q)-SHU-Rad51-Rad51 bound to ssDNA with ATP. Composite map Method: EM (single particle) / Resolution: 3.4 Å EMDB-72261: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on Rad55/Rad57 Method: EM (single particle) / Resolution: 3.72 Å EMDB-72262: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on SHU Method: EM (single particle) / Resolution: 5.75 Å EMDB-72263: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Local map focused on Rad51 Method: EM (single particle) / Resolution: 3.68 Å EMDB-72264: Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP. Composite map Method: EM (single particle) / Resolution: 3.7 Å EMDB-72268: Rad55-Rad57-SHU homologous recombination complex. Local refinement on Rad55/Rad57 Method: EM (single particle) / Resolution: 2.65 Å EMDB-72269: Rad55-Rad57-SHU homologous recombination complex. Local refinement on SHU Method: EM (single particle) / Resolution: 3.34 Å EMDB-72270: Rad55-Rad57-SHU homologous recombination complex. Composite map Method: EM (single particle) / Resolution: 2.74 Å
Chemicals	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogueYM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM
Source	saccharomyces cerevisiae (brewer's yeast) saccharomyces cerevisiae awri1631 (yeast) synthetic construct (others)
Keywords	DNA BINDING PROTEIN/DNA / Homologous Recombination Complex / Rad51 Paralog Complex / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN/Transferase / DNA BINDING PROTEIN-Transferase complex