EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	OC43 clinical isolate spike proteins have distinct carbohydrate-binding properties.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年5月16日
著者	Zaky Hassan / Min Jin / Ying Liu / Zhijie Li / Alan H M Wong / Marc Desforges / Adam Forman / Mark Nitz / Tarini Gunawardena / Theo J Moraes / Masahiro Narimatsu / Jeffrey L Wrana / Hai Yu / Xi Chen / James M Rini /
PubMed 要旨	The human coronavirus HCoV-OC43 (OC43) is the most widespread of the four common cold-causing seasonal coronaviruses, and tissue culture-adapted strains of it have been used for ~50 years. ...The human coronavirus HCoV-OC43 (OC43) is the most widespread of the four common cold-causing seasonal coronaviruses, and tissue culture-adapted strains of it have been used for ~50 years. Nevertheless, clinical isolates of OC43 differ from tissue culture-adapted OC43 in ways that call into question the value of the latter as a model. Among these are differences in their entry mechanisms and the activities of their hemagglutinin-esterases (HE). We now show that the spike proteins of OC43 clinical isolates differ from that of the tissue culture-adapted reference strain (OC43-Lab) in their carbohydrate-binding properties and ability to bind mucins, decoy receptors cleaved by the HE. We also show that, unlike HCoV-HKU1 (HKU1), they do not bind with high affinity and specificity the 9-O-acetylated α2-8-linked disialic acid moiety implicated in viral entry for OC43-Lab and HKU1. The spike proteins of the OC43 clinical isolates possess two inserts, not found in OC43-Lab, that flank the carbohydrate-binding site. Our structural analysis of a representative clinical isolate shows that insert-2 is a determinant of these specificity differences and that the carbohydrate-binding site undergoes conformational changes on carbohydrate binding. These structural features are shared by HKU1 and suggest common mechanisms for adaptation to the human sialoglycome.
リンク	Nat Commun / PubMed:42143065 / PubMed Central
手法	EM (単粒子)
解像度	1.68 - 2.05 Å
構造データ	70900 9ovk EMDB-70900, PDB-9ovk: Cryo-EM structure of HCoV-OC43-Lab Spike glycoprotein in complex with 9O-acetyl GD3 sialoglycan (D1 domain local refine) 手法: EM (単粒子) / 解像度: 1.7 Å 70901 9ovl EMDB-70901, PDB-9ovl: Cryo-EM structure of HCoV-OC43-Lab Spike glycoprotein in complex with 9O-acetyl GD3 sialoglycan 手法: EM (単粒子) / 解像度: 1.68 Å 70902 9ovm EMDB-70902, PDB-9ovm: Cryo-EM structure of HCoV-OC43-C2 Spike glycoprotein (local refined D1 domain) 手法: EM (単粒子) / 解像度: 2.05 Å 70903 9ovn EMDB-70903, PDB-9ovn: Cryo-EM structure of HCoV-OC43-C2 Spike glycoprotein 手法: EM (単粒子) / 解像度: 1.93 Å 70904 9ovo EMDB-70904, PDB-9ovo: Cryo-EM structure of HCoV-OC43-C2 Spike glycoprotein in complex with 9O-acetyl GD3 sialoglycan (Local refined D1 domain) 手法: EM (単粒子) / 解像度: 1.98 Å 70905 9ovp EMDB-70905, PDB-9ovp: Cryo-EM structure of HCoV-OC43-C2 Spike glycoprotein in complex with 9O-acetyl GD3 sialoglycan 手法: EM (単粒子) / 解像度: 1.89 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose PDB-1ar1: Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment ChemComp-HOH: WATER
由来	human coronavirus oc43 (ヒトコロナウイルス OC43)
キーワード	VIRAL PROTEIN / spike glycoprotein ectodomain / proline stablized