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-Structure paper
| タイトル | Structure of a new capsid form and comparison with A-, B-, and C-capsids clarify herpesvirus assembly. |
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| ジャーナル・号・ページ | J Virol, Vol. 99, Issue 7, Page e0050425, Year 2025 |
| 掲載日 | 2025年7月22日 |
 著者 | Alexander Stevens / Saarang Kashyap / Ethan Crofut / Ana Lucia Alvarez-Cabrera / Jonathan Jih / Yun-Tao Liu / Z Hong Zhou /  |
| PubMed 要旨 | Three capsid types have been recognized from the nuclei of herpesvirus-infected cells: empty A-capsids, scaffolding-containing B-capsids, and DNA-filled C-capsids. Despite progress in determining ...Three capsid types have been recognized from the nuclei of herpesvirus-infected cells: empty A-capsids, scaffolding-containing B-capsids, and DNA-filled C-capsids. Despite progress in determining atomic structures of these capsids and extracellular virions in recent years, debate persists concerning the origins and temporal relationships among these capsids during capsid assembly and genome packaging. Here, we have imaged over 300,000 capsids of herpes simplex virus type 1 by cryogenic electron microscopy (cryoEM) and exhaustively classified them to characterize the structural heterogeneity of the DNA-translocating portal complex and their functional states. The resultant atomic structures reveal not only the expected A-, B-, and C-capsids but also capsids with portal vertices similar to C-capsids but no resolvable genome in the capsid lumen, which we named D-capsids. The dodecameric dsDNA-translocating portal complex varies across these capsid types in their radial positions in icosahedral capsids and exhibits structural dynamics within each capsid type. In D-capsids, terminal DNA density exists in multiple conformations including one reminiscent of that in C-capsids, suggesting D-capsids are products of failed DNA retention. This interpretation is supported by varying amounts of DNA outside individual D-capsids and by the correlation of capsid counts observed of infected cell nuclei and those after purification. Additionally, an "anchoring" segment of the scaffold protein is resolved interacting with the portal baskets of A- and B-capsids but not D- and C-capsids. Taken together, our data indicate that A-capsids arise from failed DNA packaging and D-capsids from failed genome retention, clarifying the origins of empty capsids in herpesvirus assembly.IMPORTANCEAs the prototypical herpesvirus, herpes simplex virus 1 (HSV-1) exhibits a global seroprevalence of 67% and approaching 90% in some localities. Herpesvirus infections can cause devastating cancers and birth defects, with HSV-1 infections leading to cold sores among the general population worldwide and blindness in developing nations. Here, we present atomic structures of the capsids sorted out from the nuclear isolates of HSV-1 infected cells, including the previously recognized A-, B-, and C-capsids, as well as the newly identified D-capsid. The structures show the details of protein-protein and protein-DNA interactions within each capsid type and the positional and interactional variability of the viral DNA-translocating portal vertex among these capsids. Importantly, our findings suggest that A-capsids are products of failed dsDNA packaging and D-capsids of failed genome retention. Together, the high-resolution 3D structures clarify the processes of genome packaging, maintenance, and ejection during capsid assembly, which are conserved across all herpesviruses. |
 リンク | J Virol / PubMed:40607812 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.4 - 9.2 Å |
| 構造データ | EMDB-70678, PDB-9op4: EMDB-70679, PDB-9op5: EMDB-70682, PDB-9op8:  EMDB-70683: HSV-1 A-capsid portal vertex reconstruction with C5 symmetry  EMDB-70684: HSV-1 B-capsid portal vertex reconstruction with C5 symmetry EMDB-70687, PDB-9opb:  EMDB-70688: HSV-1 B-capsid portal vertex reconstruction with C1 symmetry  EMDB-70689: HSV-1 A-capsid reconstruction  EMDB-70690: HSV-1 B-capsid reconstruction EMDB-70691, PDB-9opc:  EMDB-70692: C Capsid C1 Portal Vertex  EMDB-70693: HSV-1 C-capsid reconstruction  EMDB-70694: HSV-1 D-capsid reconstruction  EMDB-70695: HSV-1 B-capsid reconstruction with global scaffold arrangement  EMDB-70696: C- capsid portal genome  EMDB-70698: HSV-1 D-capsid portal vertex reconstruction with C1 symmetry  EMDB-70699: A-Capsid C1 Portal Vertex  EMDB-70701: C-Capsid C5 Portal Vertex EMDB-70725, PDB-9opv: |
| 由来 |
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 キーワード | VIRAL PROTEIN / Complex |
human alphaherpesvirus 1 strain kos (ヘルペスウイルス)