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TitleA neutralizing human antibody induces movement of the HCoV-229E receptor binding domain.
Journal, issue, pagesCommun Biol, Year 2025
Publish dateDec 31, 2025
AuthorsMera F Liccione / Trevor Scobey / Heather M Froggatt / Camryn Pajon / Boyd L Yount / Qi Yin / Taylor N Spence / Emmanuel B Walter / Kevin O Saunders / Robert J Edwards / Ralph S Baric / Barton F Haynes / Derek W Cain / Timothy P Sheahan / Daniel Wrapp /
PubMed AbstractHCoV-229E is an endemic Alphacoronavirus that typically causes common cold-like disease in most healthy adults, but can also cause severe respiratory disease in the very young and the elderly. ...HCoV-229E is an endemic Alphacoronavirus that typically causes common cold-like disease in most healthy adults, but can also cause severe respiratory disease in the very young and the elderly. Although the virus was discovered over sixty years ago and undergoes continuous antigenic drift, remarkably little is known about the humoral immune response to HCoV-229E infection. Here we report the isolation of two receptor binding domain-targeting neutralizing human antibodies raised in response to natural HCoV-229E infection. One of these, DH1533, potently neutralizes HCoV-229E, binds to spike with sub-nanomolar affinity and prevents the association between the RBD and the host cell receptor aminopeptidase N. Structural characterization of this antibody bound to HCoV-229E spike delineated a neutralization-sensitive epitope on the RBD and revealed that DH1533 induces conformational flexibility in neighboring RBDs, reminiscent of the "up-and-down" kinetics observed in the related Betacoronavirus spikes. These findings provide insight into the humoral immune response to HCoV-229E infection and will serve as a guide for the design of future therapeutic interventions.
External linksCommun Biol / PubMed:41476125
MethodsEM (single particle)
Resolution2.82 - 3.38 Å
Structure data

70440

9ofo

EMDB-70440, PDB-9ofo:
HCoV-229E S2P bound by three DH1533 Fabs
Method: EM (single particle) / Resolution: 2.82 Å

70441

9ofp

EMDB-70441, PDB-9ofp:
HCoV-229E S2P bound by two DH1533 Fabs
Method: EM (single particle) / Resolution: 3.16 Å

70442

9ofq

EMDB-70442, PDB-9ofq:
HCoV-229E S2P bound by one DH1533 Fab
Method: EM (single particle) / Resolution: 2.88 Å

EMDB-70507: HCoV-229E S2P bound by one DH1533 Fab, consensus map
Method: EM (single particle) / Resolution: 2.88 Å

EMDB-70508: HCoV-229E S2P bound by one DH1533 Fab, focused map
Method: EM (single particle) / Resolution: 3.38 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • human coronavirus 229e
  • homo sapiens (human)
KeywordsVIRAL PROTEIN / coronavirus spike / antibody / HCoV-229E / alphacoronavirus

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