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TitleThe structure of a thermostable phage's portal vertex and neck complex illuminates the headful maturation mechanism.
Journal, issue, pagesJ Mol Biol, Page 169641, Year 2026
Publish dateJan 14, 2026
AuthorsEmma L Sedivy / Emily Agnello / Julia E Hobaugh / Rakeyah Ahsan / Kangkang Song / Chen Xu / Brian A Kelch /
PubMed AbstractViruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged ...Viruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged into its capsid shell through the portal complex. The portal complex then closes to retain DNA and connects to the tail, which is required for host recognition and infection. The trigger to stop pumping DNA and assemble the mature virus has been a longstanding conundrum in the field. We determined the structure of the portal, the proteins that connect it to the tail, and portal vertex in the hyperthermophilic phage Oshimavirus using cryo-Electron Microscopy (cryo-EM). We find highly intertwined loop structures, like in a wicker basket, potentially stabilizing the portal vertex against high temperatures. Moreover, we observe that the portal protrudes from the capsid in mature virions. We propose that portal is repositioned by packaged DNA, forming a pressure-sensitive switch that terminates genome packaging and triggers tail attachment in headful phages.
External linksJ Mol Biol / PubMed:41544933
MethodsEM (single particle)
Resolution2.3 - 7.3 Å
Structure data

70278

9oab

EMDB-70278, PDB-9oab:
C3 reconstruction of the thermophilic bacteriophage P74-26 Neck
Method: EM (single particle) / Resolution: 2.6 Å

70279

9oac

EMDB-70279, PDB-9oac:
C5 reconstruction of the thermophilic bacteriophage P74-26 Portal Vertex
Method: EM (single particle) / Resolution: 2.6 Å

70280

9oad

EMDB-70280, PDB-9oad:
C1 reconstruction of the thermophilic bacteriophage P74-26 Portal and Portal Vertex
Method: EM (single particle) / Resolution: 3.56 Å

70281

9oae

EMDB-70281, PDB-9oae:
Composite reconstruction of the thermophilic bacteriophage P74-26 Neck and Portal Vertex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-70282: C3 reconstruction of the thermophilic bacteriophage P74-26 Capsid-less Neck
Method: EM (single particle) / Resolution: 2.74 Å

EMDB-70283: C12 reconstruction of the thermophilic bacteriophage P74-26 Neck
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-70284: C12 reconstruction of the thermophilic bacteriophage P74-26 Collar-less Neck
Method: EM (single particle) / Resolution: 2.6 Å

72288

9q7a

EMDB-72288, PDB-9q7a:
dsDNA in the central channel of the bacteriophage P74-26 neck
Method: EM (single particle) / Resolution: 4.54 Å

EMDB-72289: Asymmetric reconstruction of filled phage capsids lacking neck and tail
Method: EM (single particle) / Resolution: 7.3 Å

EMDB-72290: Asymmetric reconstruction of filled phage capsids with broken tails
Method: EM (single particle) / Resolution: 5.8 Å

Source
  • oshimavirus p7426
KeywordsVIRUS / bacteriophage / thermophilic / Neck / Portal / Capsid / VIRUS/DNA / VIRUS-DNA complex

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