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-Structure paper
Title | Cryo-electron microscopy structure of the filamentous bacteriophage IKe. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 116, Issue 12, Page 5493-5498, Year 2019 |
Publish date | Mar 19, 2019 |
Authors | Jingwei Xu / Nir Dayan / Amir Goldbourt / Ye Xiang / |
PubMed Abstract | The filamentous bacteriophage IKe infects cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 Å. The major ...The filamentous bacteriophage IKe infects cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 Å. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a ∼68-Å-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 Å and a twist of 38.52°. The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, π-π interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained. |
External links | Proc Natl Acad Sci U S A / PubMed:30819888 / PubMed Central |
Methods | EM (helical sym.) |
Resolution | 3.4 - 4.5 Å |
Structure data | EMDB-6993: The cryo-EM structure of filamentous bacteriophage IKe applied with C5 and helical parameters. EMDB-6994: |
Source |
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Keywords | VIRAL PROTEIN / filamentous bacteriophage / major coat protein |