Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Ligand binding and conformational changes of SUR1 subunit in pancreatic ATP-sensitive potassium channels.
Journal, issue, pages	Protein Cell, Vol. 9, Issue 6, Page 553-567, Year 2018
Publish date	Mar 28, 2018
Authors	Jing-Xiang Wu / Dian Ding / Mengmeng Wang / Yunlu Kang / Xin Zeng / Lei Chen /
PubMed Abstract	ATP-sensitive potassium channels (K) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic K channels control insulin release and regulate ...ATP-sensitive potassium channels (K) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic K channels control insulin release and regulate metabolism at the whole body level. They are implicated in many metabolic disorders and diseases and are therefore important drug targets. Here, we present three structures of pancreatic K channels solved by cryo-electron microscopy (cryo-EM), at resolutions ranging from 4.1 to 4.5 Å. These structures depict the binding site of the antidiabetic drug glibenclamide, indicate how Kir6.2 (inward-rectifying potassium channel 6.2) N-terminus participates in the coupling between the peripheral SUR1 (sulfonylurea receptor 1) subunit and the central Kir6.2 channel, reveal the binding mode of activating nucleotides, and suggest the mechanism of how Mg-ADP binding on nucleotide binding domains (NBDs) drives a conformational change of the SUR1 subunit.
External links	Protein Cell / PubMed:29594720 / PubMed Central
Methods	EM (single particle)
Resolution	4.11 - 6.1 Å
Structure data	6831 5yke EMDB-6831, PDB-5yke: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on TM at 4.11A) Method: EM (single particle) / Resolution: 4.11 Å 6832 5ykf EMDB-6832, PDB-5ykf: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (3D class1 at 4.33A) Method: EM (single particle) / Resolution: 4.33 Å 6833 5ykg EMDB-6833, PDB-5ykg: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (Class2 at 4.57A) Method: EM (single particle) / Resolution: 4.57 Å 6847 5yw7 EMDB-6847, PDB-5yw7: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on SUR1 ABC transporter module at 4.4A) Method: EM (single particle) / Resolution: 4.4 Å 6848 5yw8 EMDB-6848, PDB-5yw8: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (all particles at 4.4A) Method: EM (single particle) / Resolution: 4.4 Å 6849 5yw9 EMDB-6849, PDB-5yw9: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (class1 5.0A) Method: EM (single particle) / Resolution: 5.0 Å 6850 5ywa EMDB-6850, PDB-5ywa: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (CTD class 2 at 6.1A) Method: EM (single particle) / Resolution: 6.1 Å 6851 5ywb EMDB-6851, PDB-5ywb: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (CTD class2 at 5.2A) Method: EM (single particle) / Resolution: 5.2 Å 6852 5ywc EMDB-6852, PDB-5ywc: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (CTD class1 at 4.3A) Method: EM (single particle) / Resolution: 4.3 Å 6853 5ywd EMDB-6853, PDB-5ywd: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (focused refinement of SUR1 ABC transporter module at 4.22A) Method: EM (single particle) / Resolution: 4.2 Å
Chemicals	ChemComp-GBM: 5-chloro-N-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide / medicationYM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogueYM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG*: Unknown entry
Source	mus musculus (house mouse) mesocricetus auratus (golden hamster)
Keywords	MEMBRANE PROTEIN / KATP / channel / glibenclamide / sulfonylurea