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TitleThe Conserved GTPase LepA May Contribute to the Final Proper Stabilization of the 3' Domain of the 30S Subunit During Ribosome Assembly.
Journal, issue, pagesInt J Mol Sci, Vol. 27, Issue 1, Year 2026
Publish dateJan 3, 2026
AuthorsOlesya Kravchenko / Elena Maksimova / Timur Baymukhametov / Irina Eliseeva / Elena Stolboushkina /
PubMed AbstractThe function of the highly conserved GTPase LepA, a homolog of elongation factor EF-G, remains unknown in translation. However, there is biochemical data that it implicates in the 30S ribosomal ...The function of the highly conserved GTPase LepA, a homolog of elongation factor EF-G, remains unknown in translation. However, there is biochemical data that it implicates in the 30S ribosomal subunit biogenesis. Here, using cryo-electron microscopy, we characterized 30S subunits isolated from an strain with a deleted gene. The cryo-EM maps for ∆ 30S particles were divided into classes corresponding to consecutive assembly intermediates: from particles characterized by unformed helices h44/h45 of the central decoding center (CDR) and highly flexible head, through intermediates with a distorted CDR and a partial stabilization of the head, to near-mature 30S subunits with correctly docked h44 in the CDR, accessible 3' end of 16S rRNA for translation but significant flexibility in head domain. Cryo-EM analysis of Δ 30S intermediates revealed that they predominantly proceed to nearly mature functional state and exhibit suboptimal flexibility in the head domain. This finding suggests that LepA likely contributes to the final proper stabilization of the 3' domain of the 30S subunit during ribosome assembly.
External linksInt J Mol Sci / PubMed:41516366 / PubMed Central
MethodsEM (single particle)
Resolution2.1 - 3.0 Å
Structure data

EMDB-67195: E.coli delta lepA 30S ribosomal subunit class A
Method: EM (single particle) / Resolution: 2.25 Å

EMDB-67196: E.coli delta lepA 30S ribosomal subunit class B
Method: EM (single particle) / Resolution: 2.27 Å

EMDB-67197: E.coli delta lepA 30S ribosomal subunit class C
Method: EM (single particle) / Resolution: 2.12 Å

EMDB-67198: E.coli delta lepA 30S ribosomal subunit class A, head domain
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-67199: E.coli delta lepA 30S ribosomal subunit class B, head domain
Method: EM (single particle) / Resolution: 2.66 Å

EMDB-67200: E.coli delta lepA 30S ribosomal subunit class C, head domain
Method: EM (single particle) / Resolution: 2.34 Å

67201

9xtc

EMDB-67201, PDB-9xtc:
E.coli delta lepA 30S ribosomal subunit class C, body domain
Method: EM (single particle) / Resolution: 2.1 Å

67202

9xtd

EMDB-67202, PDB-9xtd:
E.coli delta lepA 30S ribosomal subunit class B, body domain
Method: EM (single particle) / Resolution: 2.26 Å

67203

9xte

EMDB-67203, PDB-9xte:
E.coli delta lepA 30S ribosomal subunit class A, body domain
Method: EM (single particle) / Resolution: 2.24 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsRIBOSOME / 30S subunit maturationk / LepA / cryo-EM / ribosome assembly / 30S subunit maturation

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