EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	How Sup35 monomer conformation and amyloid fibril polymorphism determine yeast strain phenotypes.
ジャーナル・号・ページ	Res Sq, Year 2025
掲載日	2025年11月3日
著者	Motomasa Tanaka / Takashi Nomura / David Boyer / Yusuke Komi / Peng Ge / Rodrigo A Maillard / Piere Rodriguez / Atsushi Yamagata / Mikako Shirouzu / Giuseppe Legname / Bruno Samori / David Eisenberg /
PubMed 要旨	In the [ ] prion system, the yeast prion protein Sup35 can form structurally distinct amyloid fibrils that lead to distinct transmissible prion states, or strains. However, our understanding of how ...In the [ ] prion system, the yeast prion protein Sup35 can form structurally distinct amyloid fibrils that lead to distinct transmissible prion states, or strains. However, our understanding of how different Sup35 fibril structures arise and translate to phenotypic variations is limited. Here, using cryo-EM and single-monomer force spectroscopy with optical tweezers, we reveal the structural basis of yeast prion propagation in four wild-type and S17R mutant variants of Sup35 that underlie different [ ] strains. Cryo-EM structures show that the four variants form strikingly distinct fibril structures, which exhibit varying stability and chaperone-accessibility. Force spectroscopy suggests the different distinct fibril structures are derived from distinct monomer conformational ensembles. Further, cryo-EM structures indicate that prion strain strength is correlated with enhanced fibril propagation caused by a combination of low fibril stability and a large separation between the Sup35 fibril core and the Ssa1/Sis1 chaperone-binding region. These results provide a structure-based mechanism for the yeast prion strain phenomenon with implications for understanding amyloid propagation in human neurodegenerative diseases.
リンク	Res Sq / PubMed:41282265 / PubMed Central
手法	EM (らせん対称)
解像度	2.2 - 3.1 Å
構造データ	66703 9xbk EMDB-66703, PDB-9xbk: Cryo-EM structure of Sup35NM S17R fibril formed at 4 degrees (S17R4N) 手法: EM (らせん対称) / 解像度: 2.4 Å 66704 9xbl EMDB-66704, PDB-9xbl: Cryo-EM structure of Sup35NM S17R fibril formed at 37 degrees (S17R37N) 手法: EM (らせん対称) / 解像度: 2.4 Å 66705 9xbm EMDB-66705, PDB-9xbm: Cryo-EM structure of Sup35NM S17R fibril formed at 37 degrees (S17R37C) 手法: EM (らせん対称) / 解像度: 2.4 Å 66706 9xbn EMDB-66706, PDB-9xbn: Cryo-EM structure of Sup35NM fibril formed at 4 degrees (Sc4) 手法: EM (らせん対称) / 解像度: 3.1 Å 66707 9xbo EMDB-66707, PDB-9xbo: Cryo-EM structure of Sup35NM fibril formed at 37 degrees (Sc37) 手法: EM (らせん対称) / 解像度: 3.1 Å 66708 9xbp EMDB-66708, PDB-9xbp: Cryo-EM structure of Sup35NM S17R fibril formed at 4 degrees (S17R4C) 手法: EM (らせん対称) / 解像度: 2.2 Å
化合物	ChemComp-HOH: WATER
由来	saccharomyces cerevisiae (パン酵母)
キーワード	PROTEIN FIBRIL / Yeast / Sup35NM / Amyloid