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TitleStructural insights into a PSI-ACPI supercomplex from a cryptophyte alga Chroomonas placoidea.
Journal, issue, pagesPhotosynth Res, Vol. 164, Issue 1, Page 2, Year 2025
Publish dateDec 20, 2025
AuthorsXingyue Li / Zhiyuan Mao / Zhenhua Li / Liangliang Shen / Xiaoyi Li / Yanyan Yang / Wenda Wang / Tingyun Kuang / Jian-Ren Shen / Guangye Han /
PubMed AbstractCryptophyte algae are an important group of photosynthetic eukaryotes and originated evolutionarily from a secondary endosymbiosis with a red algal ancestor. Photosystem I (PSI) of cryptophyte algae ...Cryptophyte algae are an important group of photosynthetic eukaryotes and originated evolutionarily from a secondary endosymbiosis with a red algal ancestor. Photosystem I (PSI) of cryptophyte algae binds alloxanthin-chlorophyll a/c-binding proteins (ACPs) equivalent to the light harvesting complexes (LHCs) of green algae and higher plants. Here, we report a high resolution (2.25 Å) structure of the PSI-ACPI supercomplex purified from the cryptophyte Chroomonas placoidea (C. placoidea) by cryo-electron microscopy. The PSI-ACPI supercomplex consists of a monomeric PSI core, 14 ACPI subunits and a Chl-binding subunit, CCPI-S. The 14 ACPI subunits are divided into two layers, with an inner layer including 11 ACPIs, and an outer layer consisting of 3 ACPIs. The CCPI-S mediates the connections and energy transfer between the outer and inner ACPI layers. Due to the current higher resolution, a unique PsaQ subunit bound at the lumenal side was identified in the PSI core. Two new pigment molecules Chl a856PsaA and Mon 627ACPI − 8 as well as 18 new lipids were identified, compared to the previously reported PSI-ACPI structure from the cryptophyte alga C. placoidea (Zhao et al. 2023). These lipids mediate the interactions of subunits and the assembly of PSI-ACPI. These results provide structural insights for the assembly of the PSI-ACPI complex and pathways of light-energy absorption and transfer in cryptophyte PSI-ACPI, as well as the possible adaptation mechanisms to fluctuating light conditions.
External linksPhotosynth Res / PubMed:41420767
MethodsEM (single particle)
Resolution2.25 Å
Structure data

66373

9wyp

EMDB-66373, PDB-9wyp:
The PSI-ACPI supercomplex from the cryptophyte Chroomonas placoidea
Method: EM (single particle) / Resolution: 2.25 Å

Chemicals

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-KC2:
Chlorophyll c2

ChemComp-II0:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E})-3,7,12,16-tetramethyl-18-[(4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol

ChemComp-II3:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol

ChemComp-IHT:
(1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-8CT:
(6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-PQN:
PHYLLOQUINONE

Source
  • chroomonas placoidea (eukaryote)
KeywordsPHOTOSYNTHESIS / STRUCTURAL PROTEIN

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