EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 7758, Year 2025
掲載日	2025年8月20日
著者	Shivani Sharma / Shalini Mishra / Samudrala Gourinath / Prem S Kaushal /
PubMed 要旨	Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with ...Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with poor sanitation conditions, and it remains the fourth leading cause of death due to a protozoan infection. E. histolytica life cycle spans between an infective 'cyst stage' and an active disease-causing 'trophozoite stage'. We have isolated ribosomes from the trophozoite stage of E. histolytica. Here, we report single particle cryo-EM structures of the 53S ribosome large subunit (LSU), and 75S associated ribosomes, with P-tRNA, A/P and P/E tRNAs, and with paromomycin antibiotic, at 2.8 Å to 3.4 Å resolution. The E. histolytica possesses a reduced ribosome with a conserved core, and the periphery evolved with species-specific unique features. The most notable features are the presence of the rRNA triple helix near the peptide exit tunnel, the expansion segment H88ES102 near the exit site on LSU, and unique insertions in r-proteins. Furthermore, the 75S ribosome paromomycin complex structure provides the atomic details of its interactions. These structures provide insights into the evolutionary adaptation of the E. histolytica translational machinery and may be explored further for amoebicidal therapeutic intervention.
リンク	Nat Commun / PubMed:40835601 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.4 Å
構造データ	64694 9v1i EMDB-64694, PDB-9v1i: Cryo- EM structure of ribosomal large subunit (LSU) from Entamoeba histolytica at 2.8 angstrom resolution 手法: EM (単粒子) / 解像度: 2.8 Å 64695 9v1j EMDB-64695, PDB-9v1j: Cryo- EM structure of small subunit (body) of 75S ribosome with P- tRNA from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.1 Å 64696 9v1k EMDB-64696, PDB-9v1k: Cryo- EM structure of small subunit (head) of 75S ribosome with P- tRNA from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.4 Å 64697 9v1l EMDB-64697, PDB-9v1l: Cryo- EM structure of large subunit (LSU) of 75S ribosome with P- tRNA from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.0 Å 64713 9v21 EMDB-64713, PDB-9v21: Cryo- EM structure of 75S ribosome with P- tRNA from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.0 Å 64716 9v24 EMDB-64716, PDB-9v24: Cryo- EM structure of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.1 Å 64717 9v25 EMDB-64717, PDB-9v25: Cryo- EM structure of large subunit (LSU) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.0 Å 64718 9v26 EMDB-64718, PDB-9v26: Cryo- EM structure of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica bound to antibiotic paromomycin 手法: EM (単粒子) / 解像度: 3.1 Å 64719 9v27 EMDB-64719, PDB-9v27: Cryo- EM structure of small subunit (body) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.21 Å 64720 9v28 EMDB-64720, PDB-9v28: Cryo- EM structure of small subunit (head) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica 手法: EM (単粒子) / 解像度: 3.4 Å 64721 9v29 EMDB-64721, PDB-9v29: Cryo- EM structure of small subunit (body) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica bound to antibiotic paromomycin 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-PAR: PAROMOMYCIN / パロモマイシン / Antimicrobial, 薬剤*YM
由来	entamoeba histolytica hm-1:imss (赤痢アメーバ)
キーワード	RIBOSOME / LSU / Entamoeba histolytica / 53S / SSU-body / SSU-head / P-tRNA / LSU with P-tRNA CCA end / 75S Ribosome / A/P-tRNA / P/E-tRNA / Large Subunit of Ribosome / 75S Ribosome-PAR complex / SSU-body of 75S Ribosome / SSU-head of 75S Ribosome / SSU-body of 75S Ribosome-PAR complex