Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin-specific chaperones.
Journal, issue, pages	Science, Vol. 390, Issue 6772, Page eady2708, Year 2025
Publish date	Oct 30, 2025
Authors	Yeonjae Seong / Hyunmin Kim / Kyumi Byun / Yeon-Woo Park / Soung-Hun Roh /
PubMed Abstract	Microtubule assembly requires a set of chaperones known as tubulin-binding cofactors (TBCs). We used cryo-electron microscopy to visualize how human TBCD, TBCE, TBCC, and guanosine triphosphatase ...Microtubule assembly requires a set of chaperones known as tubulin-binding cofactors (TBCs). We used cryo-electron microscopy to visualize how human TBCD, TBCE, TBCC, and guanosine triphosphatase (GTPase) Arl2 mediate αβ-tubulin assembly and disassembly. We captured multiple conformational states, revealing how TBCs orchestrate tubulin heterodimer biogenesis. TBCD stabilizes monomeric β-tubulin and scaffolds the other cofactors. Guanosine triphosphate (GTP) binding to Arl2 induces conformational changes that toggle the complex between assembly and disassembly. TBCD and TBCE guide α- and β-tubulin into a partially assembled interface, and TBCC, acting as a molecular clamp, completes the heterodimer. TBCD also functions as a GTPase activating protein for β-tubulin. β-tubulin GTP hydrolysis is coupled to Arl2's GTPase activity, establishing a checkpoint that ensures that only fully matured heterodimers proceed. These findings provide a structural framework for tubulin heterodimer biogenesis and recycling, supporting cytoskeletal proteostasis.
External links	Science / PubMed:41166473
Methods	EM (single particle)
Resolution	2.14 - 3.59 Å
Structure data	EMDB-63376: Consensus map of the TBC-DE-Arl2-beta-tubulin complex with GTP Method: EM (single particle) / Resolution: 2.45 Å EMDB-63377: Local refinement map of Arl2 in the TBC-DE-Arl2-beta-tubulin-GTP complex Method: EM (single particle) / Resolution: 2.56 Å EMDB-63378: Local refinement map of AR3 domain in the TBC-DE-Arl2-beta-tubulin-GTP complex Method: EM (single particle) / Resolution: 2.41 Å EMDB-63546: Consensus map of the TBC-DE-Arl2-alpha-beta-tubulin complex with GTP Method: EM (single particle) / Resolution: 2.55 Å EMDB-63547: Local refinement map of TBCE and alpha-tubulin in the TBC-DE-Arl2-alpha-beta-tubulin-GTP complex Method: EM (single particle) / Resolution: 2.7 Å EMDB-63548: Local refinement map of Arl2 and TBCD-AR1 domain in the TBC-DE-Arl2-alpha-beta-tubulin-GTP complex Method: EM (single particle) / Resolution: 2.7 Å EMDB-63549: Consensus map of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Method: EM (single particle) / Resolution: 2.21 Å EMDB-63550: Local refinement map of TBCE in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.59 Å EMDB-63551: Local refinement map of TBCE and alpha-tubulin in the TBC-DEC-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.34 Å EMDB-63552: Local refinement map of TBCC-RPD and Arl2 in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.3 Å EMDB-63553: Local refinement map of TBCD and Arl2 in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.18 Å EMDB-63554: Consensus map of the TBC-DC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Method: EM (single particle) / Resolution: 2.24 Å EMDB-63555: Local refinement map of alpha-tubulin in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.37 Å EMDB-63556: Local refinement map of TBCD and Arl2 in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.23 Å EMDB-63557: Local refinement map of TBCC-RPD and Arl2 in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.34 Å EMDB-63558: Local refinement map of TBCD AR3 domain in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex Method: EM (single particle) / Resolution: 2.94 Å EMDB-63569: Consensus map of the TBC-DE-Arl2-beta-tubulin complex Method: EM (single particle) / Resolution: 2.14 Å EMDB-63570: Local refinement map of TBCE-LRRD in the TBC-DE-Arl2-beta-tubulin complex Method: EM (single particle) / Resolution: 3.59 Å 63572 9m1i EMDB-63572, PDB-9m1i: Cryo-EM structure of the TBC-D-Arl2-beta-tubulin complex Method: EM (single particle) / Resolution: 2.48 Å 63573 9m1j EMDB-63573, PDB-9m1j: Cryo-EM structure of the TBC-DE-Arl2-beta-tubulin complex Method: EM (single particle) / Resolution: 2.14 Å 63574 9m1k EMDB-63574, PDB-9m1k: Cryo-EM structure of the TBC-DE-Arl2-beta-tubulin complex with GTP Method: EM (single particle) / Resolution: 2.45 Å 63575 9m1l EMDB-63575, PDB-9m1l: Cryo-EM structure of the TBC-DE-Arl2-alpha-beta-tubulin complex with GTP Method: EM (single particle) / Resolution: 2.55 Å 63576 9m1m EMDB-63576, PDB-9m1m: Cryo-EM structure of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Method: EM (single particle) / Resolution: 2.21 Å 63577 9m1n EMDB-63577, PDB-9m1n: Cryo-EM structure of the TBC-DC-Arl2-alpha-beta-tubulin complex with GDP-AlFx Method: EM (single particle) / Resolution: 2.24 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-AF3: ALUMINUM FLUORIDE
Source	homo sapiens (human) sus scrofa (pig)
Keywords	CHAPERONE / complex