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TitleStructural insight into GPR155-mediated cholesterol sensing and signal transduction.
Journal, issue, pagesSci Bull (Beijing), Vol. 70, Issue 21, Page 3625-3637, Year 2025
Publish dateNov 15, 2025
AuthorsDelin Li / Xiaokang Zhang / Jie Feng / Yufeng Xie / Pu Han / Ming He / Lin Hao / Tianling Guo / Xiaoyi Bai / Kai Yuan / Junqing Sun / Xuefei Pang / Yan Wu / Yingxia Liu / George Fu Gao / Niu Huang / Haixia Xiao / Feng Gao /
PubMed AbstractCholesterol (CHL) serves as a building block for membrane biogenesis and a precursor to oxysterols, steroid hormones, bile acids, and vitamin D. The lysosome serves as a major sorting station for low- ...Cholesterol (CHL) serves as a building block for membrane biogenesis and a precursor to oxysterols, steroid hormones, bile acids, and vitamin D. The lysosome serves as a major sorting station for low-density lipoproteins (LDLs), which carry dietary CHL, and it is also the cellular site where the master growth regulator, the protein kinase mechanistic Target of Rapamycin Complex 1 (mTORC1), is activated. Recently, the lysosomal transmembrane protein GPR155 was reported to signals CHL sufficiency to mTORC1 through sequestration of the GTPase-activating protein towards the Rags 1 (GATOR1). Although the recently reported structures of GPR155 have revealed the CHL binding site, how the signal is transduced from the CHL binding site to the soluble parts of GPR155 and GATOR1 remains unknown. Here, with our three cryo-EM structures of GPR155 captured in different conformations in complex with CHL, complemented by long-time scale molecular dynamics simulations, the dynamic rearrangement of different domains was observed. CHL binding induces a widening of the crevice between the transporter and GPCR domains. The extending helix preceding transmembrane helix (TM) 16, which was unresolved in other structures, acts as a linkage lever that transmits the rotation of the GPCR domain to the soluble parts of GPR155 in response to CHL binding. This work not only answers the question of how CHL is sensed by GPR155, but also addresses a more profound question: how the signal perceived by the TMs regions is transduced to the LED and DEP domains.
External linksSci Bull (Beijing) / PubMed:41058362
MethodsEM (single particle)
Resolution2.79 - 5.62 Å
Structure data

63104

9lhq

EMDB-63104, PDB-9lhq:
Cryo-EM structure of GPR155 monomer in complex with cholesterol
Method: EM (single particle) / Resolution: 3.46 Å

63106

9lhv

EMDB-63106, PDB-9lhv:
Cryo-EM structure of GPR155 contracted dimer in complex with cholesterol
Method: EM (single particle) / Resolution: 2.79 Å

63107

9lhx

EMDB-63107: Cryo-EM map of GPR155 extended dimer
PDB-9lhx: Rigid fitting model of GPR155 extended dimer
Method: EM (single particle) / Resolution: 5.62 Å

Chemicals

ChemComp-CLR:
CHOLESTEROL

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / cholesterol / mTORC1 regulation / GPCR / transceptor

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