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Structure paper

TitleStructural insight into sodium ion pathway in the bacterial flagellar stator from marine .
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 122, Issue 1, Page e2415713122, Year 2025
Publish dateJan 7, 2025
AuthorsTatsuro Nishikino / Norihiro Takekawa / Jun-Ichi Kishikawa / Mika Hirose / Seiji Kojima / Michio Homma / Takayuki Kato / Katsumi Imada /
PubMed AbstractMany bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ...Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the sodium-driven stator of , namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation based on the structures. This work provides insights into the molecular mechanisms of ion specificity and conversion of the electrochemical potential into mechanical functions.
External linksProc Natl Acad Sci U S A / PubMed:39793043 / PubMed Central
MethodsEM (single particle)
Resolution3.12 - 3.43 Å
Structure data

60580

8zyv

EMDB-60580, PDB-8zyv:
Bacterial flagellar sodium-driven stator PomA5PomB2 with 100 mM NaCl
Method: EM (single particle) / Resolution: 3.12 Å

60581

8zyw

EMDB-60581, PDB-8zyw:
Bacterial flagellar sodium-driven stator PomA5PomB2 with 100 mM KCl
Method: EM (single particle) / Resolution: 3.43 Å

60584

8zyz

EMDB-60584, PDB-8zyz:
Bacterial flagellar sodium-driven stator PomA5PomB2(D24N) with 100 mM NaCl
Method: EM (single particle) / Resolution: 3.16 Å

60585

8zz0

EMDB-60585, PDB-8zz0:
Bacterial flagellar sodium-driven stator PomA5PomB2(D24N) with 100 mM KCl
Method: EM (single particle) / Resolution: 3.43 Å

60636

9ijm

EMDB-60636, PDB-9ijm:
Bacterial flagellar sodium-driven stator PomA5PomB2 with 100 mM NaCl and 0.1 mM phenamil
Method: EM (single particle) / Resolution: 3.32 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

PDB-1l2k:
Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

Source
  • vibrio alginolyticus (bacteria)
KeywordsMEMBRANE PROTEIN / bacterial flagellar stator unit / sodium ion transport / membrane protein complex / Phenamil

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