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TitleCryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state.
Journal, issue, pagesJ Mol Biol, Vol. 425, Issue 22, Page 4544-4555, Year 2013
Publish dateNov 15, 2013
AuthorsDuncan R Sousa / Scott M Stagg / M Elizabeth Stroupe /
PubMed AbstractTropomyosin (Tm) is a key factor in the molecular mechanisms that regulate the binding of myosin motors to actin filaments (F-Actins) in most eukaryotic cells. This regulation is achieved by the ...Tropomyosin (Tm) is a key factor in the molecular mechanisms that regulate the binding of myosin motors to actin filaments (F-Actins) in most eukaryotic cells. This regulation is achieved by the azimuthal repositioning of Tm along the actin (Ac):Tm:troponin (Tn) thin filament to block or expose myosin binding sites on Ac. In striated muscle, including involuntary cardiac muscle, Tm regulates muscle contraction by coupling Ca(2+) binding to Tn with myosin binding to the thin filament. In smooth muscle, the switch is the posttranslational modification of the myosin. Depending on the activation state of Tn and the binding state of myosin, Tm can occupy the blocked, closed, or open position on Ac. Using native cryogenic 3DEM (three-dimensional electron microscopy), we have directly resolved and visualized cardiac and gizzard muscle Tm on filamentous Ac in the position that corresponds to the closed state. From the 8-Å-resolution structure of the reconstituted Ac:Tm filament formed with gizzard-derived Tm, we discuss two possible mechanisms for the transition from closed to open state and describe the role Tm plays in blocking myosin tight binding in the closed-state position.
External linksJ Mol Biol / PubMed:24021812 / PubMed Central
MethodsEM (single particle)
Resolution8.0 - 15.0 Å
Structure data

5751

3j4k

EMDB-5751: Cryogenic electron microscopy of cardiac actin:tropomyosin
PDB-3j4k: Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state
Method: EM (single particle) / Resolution: 8.0 Å

EMDB-5752:
Cryogenic electron microscopy of cardiac actin:tropomyosin
Method: EM (single particle) / Resolution: 15.0 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • oryctolagus cuniculus (rabbit)
  • gallus gallus (chicken)
  • Bos taurus (cattle)
KeywordsSTRUCTURAL PROTEIN / actin / tropomyosin / coiled-coil C-state

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