Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome.
Journal, issue, pages	bioRxiv, Year 2026
Publish date	Feb 9, 2026
Authors	Florent Waltz / Philippe A Lehner / Philippe Van der Stappen / Lukas Kater / Stefan Pfeffer / Benjamin D Engel /
PubMed Abstract	Chloroplast ribosomes (chlororibosomes) synthesize the core protein components of the photosynthetic apparatus, yet their structural diversity outside flowering plants remains largely unexplored. ...Chloroplast ribosomes (chlororibosomes) synthesize the core protein components of the photosynthetic apparatus, yet their structural diversity outside flowering plants remains largely unexplored. Here, we combine in situ cryo-electron tomography (cryo-ET) with single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the chlororibosome from the unicellular green alga . Subtomogram averaging of chlororibosomes in their native environment, resolved to ~5 Å resolution and in distinct translational states, reveals particles both free in the stroma and loosely tethered to thylakoid membranes. These reconstructions uncover an additional "arm" domain on the small subunit. High-resolution single-particle reconstruction of isolated chlororibosomes to ~2.5 Å, in states bound either to the inhibitory translation factor pY or to a nascent chain-linked P-site tRNA, reveals that this domain is built primarily from extensive chloroplast-encoded insertions and extensions of conserved small subunit proteins, supported by chlororibosome-specific ribosomal proteins. The arm domain is located around the mRNA entry and exit channels, suggesting a role in stabilizing the mRNA trajectory through the small subunit and organizing chloroplast polysomes. Together, these data reveal unexpected structural variation of algal chlororibosomes and suggest that chloroplast translation has diversified substantially even among relatively closely related photosynthetic lineages.
External links	bioRxiv / PubMed:41727112 / PubMed Central
Methods	EM (subtomogram averaging) / EM (single particle)
Resolution	2.45 - 9.29 Å
Structure data	EMDB-56139: Subtomogram average of the Chlamydomonas reinhardtii chlororibosome - consensus map Method: EM (subtomogram averaging) / Resolution: 6.11 Å EMDB-56140: Subtomogram average of the Chlamydomonas reinhardtii chlororibosome - LSU focused Method: EM (subtomogram averaging) / Resolution: 5.76 Å EMDB-56141: Subtomogram average of the Chlamydomonas reinhardtii chlororibosome - SSU focused Method: EM (subtomogram averaging) / Resolution: 6.36 Å EMDB-56142: Subtomogram average of the Chlamydomonas reinhardtii chlororibosome - SSU extension focused Method: EM (subtomogram averaging) / Resolution: 8.48 Å EMDB-56143: Subtomogram average of the Chlamydomonas reinhardtii chlororibosome - chimeric map Method: EM (subtomogram averaging) / Resolution: 6.11 Å EMDB-56144: Subtomogram average of the membrane-bound Chlamydomonas reinhardtii chlororibosome Method: EM (subtomogram averaging) / Resolution: 9.29 Å EMDB-56337: Structure of the Chlamydomonas reinhardtii chlororibosome - consensus map Method: EM (single particle) / Resolution: 2.52 Å EMDB-56338: Structure of the Chlamydomonas reinhardtii chlororibosome - LSU focus Method: EM (single particle) / Resolution: 2.45 Å EMDB-56339: Structure of the Chlamydomonas reinhardtii chlororibosome - LSU CP focus Method: EM (single particle) / Resolution: 2.55 Å EMDB-56340: Structure of the Chlamydomonas reinhardtii chlororibosome - LSU L7/12 stalk focus Method: EM (single particle) / Resolution: 3.99 Å EMDB-56341: Structure of the Chlamydomonas reinhardtii chlororibosome - SSU body focus Method: EM (single particle) / Resolution: 2.57 Å EMDB-56342: Structure of the Chlamydomonas reinhardtii chlororibosome - SSU head focus Method: EM (single particle) / Resolution: 2.63 Å EMDB-56344: Structure of the Chlamydomonas reinhardtii chlororibosome - SSU head extension recenter and focus Method: EM (single particle) / Resolution: 3.94 Å EMDB-56349: Structure of the Chlamydomonas reinhardtii chlororibosome - SSU extension focus Method: EM (single particle) / Resolution: 2.98 Å 56352 9tvu EMDB-56352, PDB-9tvu: Structure of the Chlamydomonas reinhardtii chlororibosome Method: EM (single particle) / Resolution: 2.52 Å EMDB-56560: Structure of the Chlamydomonas reinhardtii chlororibosome with pY factor - consensus map Method: EM (single particle) / Resolution: 2.56 Å EMDB-56561: Structure of the Chlamydomonas reinhardtii chlororibosome with P-site tRNA - consensus map Method: EM (single particle) / Resolution: 2.84 Å EMDB-56562: Structure of the Chlamydomonas reinhardtii chlororibosome with P-site tRNA - LSU focus Method: EM (single particle) / Resolution: 2.76 Å EMDB-56563: Structure of the Chlamydomonas reinhardtii chlororibosome with pY factor - LSU focus Method: EM (single particle) / Resolution: 2.48 Å EMDB-56564: Structure of the Chlamydomonas reinhardtii chlororibosome with pY factor - focus SSU Method: EM (single particle) / Resolution: 2.63 Å EMDB-56565: Structure of the Chlamydomonas reinhardtii chlororibosome with P-site tRNA - focus SSU Method: EM (single particle) / Resolution: 2.88 Å 56567 28jw EMDB-56567, PDB-28jw: Structure of the Chlamydomonas reinhardtii chlororibosome with P-site tRNA Method: EM (single particle) / Resolution: 2.84 Å 56602 28lu EMDB-56602, PDB-28lu: Structure of the Chlamydomonas reinhardtii chlororibosome with factor pY Method: EM (single particle) / Resolution: 2.6 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-K: Unknown entry ChemComp-CLM: CHLORAMPHENICOL / antibiotic*YM
Source	chlamydomonas reinhardtii (plant)
Keywords	RIBOSOME / chloroplast / translation / chlororibosome