EMDB-56567: Structure of the Chlamydomonas reinhardtii chlororibosome with P-...

Basic information

Entry

Database: EMDB / ID: EMD-56567

• Title: Structure of the Chlamydomonas reinhardtii chlororibosome with P-site tRNA

Sample

• Complex: Chloroplast ribosome
• Protein or peptide: x 54 types
• RNA: x 8 types
• Ligand: x 3 types

• Keywords: chloroplast / ribosome / translation / chlororibosome

Function / homology

Function:

mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial translation / plastid / chloroplast / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / mitochondrion / RNA binding / cytoplasm

Domain/homology:

Ribosomal protein PSRP-3/Ycf65 / PSRP-3/Ycf65 superfamily / Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65) / Ribosomal protein L6, chloroplast / Family of unknown function (DUF5323) / TRAM domain / TRAM domain / TRAM domain profile. / : / : / S1 domain profile. / Ribosomal protein L11, bacterial-type / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / S1 domain / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L28 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L5, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L28/L24 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein S15, bacterial-type / Ribosomal protein L16 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily

Component:

Small ribosomal subunit protein uS2c / Large ribosomal subunit protein bL9c / Uncharacterized protein / Large ribosomal subunit protein uL18c / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein bL28c / Uncharacterized protein / 30S ribosomal protein S15 / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein bL17c / 30S ribosomal protein 3, chloroplastic / Uncharacterized protein / Large ribosomal subunit protein uL11c / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Small ribosomal subunit protein uS10 domain-containing protein / 50S ribosomal protein L31 / Large ribosomal subunit protein uL6 alpha-beta domain-containing protein / Uncharacterized protein / Uncharacterized protein / KOW domain-containing protein / Uncharacterized protein / 30S ribosomal protein S16, chloroplastic / Uncharacterized protein / Large ribosomal subunit protein uL3c / 50S ribosomal protein L35 / Small ribosomal subunit protein uS17c / Small ribosomal subunit protein uS9c / Small ribosomal subunit protein bS18c / Large ribosomal subunit protein uL16c / Large ribosomal subunit protein uL14c / Small ribosomal subunit protein uS12c / Large ribosomal subunit protein bL20c / Small ribosomal subunit protein uS7c / Small ribosomal subunit protein uS4c / Large ribosomal subunit protein bL36c / Small ribosomal subunit protein uS8c / Small ribosomal subunit protein uS19c / Small ribosomal subunit protein uS3c / Plastid-specific ribosomal protein-7 / Ribosomal protein S1 homologue / Small ribosomal subunit protein uS11c / Large ribosomal subunit protein uL22c / Large ribosomal subunit protein uL4c / Large ribosomal subunit protein uL5c / Large ribosomal subunit protein uL2c / Large ribosomal subunit protein uL23c / Small ribosomal subunit protein uS14c

• Biological species: Chlamydomonas reinhardtii (plant)
• Method: single particle reconstruction / cryo EM / Resolution: 2.84 Å
• Authors: Waltz F / Kater L / Engel BD

Funding support

Switzerland, 2 items

Organization	Grant number	Country
Swiss National Science Foundation	216094	Switzerland
Swiss State Secretariat for Education, Research and Innovation	M822.00045	Switzerland

• Citation: Journal: bioRxiv / Year: 2026; Title: Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome.; Authors: Florent Waltz / Philippe A Lehner / Philippe Van der Stappen / Lukas Kater / Stefan Pfeffer / Benjamin D Engel / ; Abstract: Chloroplast ribosomes (chlororibosomes) synthesize the core protein components of the photosynthetic apparatus, yet their structural diversity outside flowering plants remains largely unexplored. Here, we combine in situ cryo-electron tomography (cryo-ET) with single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the chlororibosome from the unicellular green alga . Subtomogram averaging of chlororibosomes in their native environment, resolved to ~5 Å resolution and in distinct translational states, reveals particles both free in the stroma and loosely tethered to thylakoid membranes. These reconstructions uncover an additional "arm" domain on the small subunit. High-resolution single-particle reconstruction of isolated chlororibosomes to ~2.5 Å, in states bound either to the inhibitory translation factor pY or to a nascent chain-linked P-site tRNA, reveals that this domain is built primarily from extensive chloroplast-encoded insertions and extensions of conserved small subunit proteins, supported by chlororibosome-specific ribosomal proteins. The arm domain is located around the mRNA entry and exit channels, suggesting a role in stabilizing the mRNA trajectory through the small subunit and organizing chloroplast polysomes. Together, these data reveal unexpected structural variation of algal chlororibosomes and suggest that chloroplast translation has diversified substantially even among relatively closely related photosynthetic lineages.

History

Deposition	Feb 4, 2026	-
Header (metadata) release	Apr 1, 2026	-
Map release	Apr 1, 2026	-
Update	Apr 1, 2026	-
Current status	Apr 1, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_56567.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.0631 Å

Density

Contour Level	By AUTHOR: 0.0707
Minimum - Maximum	-0.17389135 - 0.7777412
Average (Standard dev.)	0.0015216351 (±0.015225294)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 544.3072 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Chloroplast ribosome

• Entire: Name: Chloroplast ribosome

Components

• Complex: Chloroplast ribosome
• Protein or peptide: cL38/PSRP6
• Protein or peptide: bL33c
• Protein or peptide: bL34c
• Protein or peptide: 50S ribosomal protein L35
• Protein or peptide: Large ribosomal subunit protein bL36c
• Protein or peptide: Large ribosomal subunit protein uL2c
• Protein or peptide: Large ribosomal subunit protein uL3c
• Protein or peptide: Large ribosomal subunit protein uL4c
• Protein or peptide: Large ribosomal subunit protein uL5c
• Protein or peptide: Plastid ribosomal protein L6
• Protein or peptide: Large ribosomal subunit protein bL9c
• Protein or peptide: uL10c
• Protein or peptide: Large ribosomal subunit protein uL11c
• Protein or peptide: uL13c
• Protein or peptide: Large ribosomal subunit protein uL14c
• Protein or peptide: uL15c
• Protein or peptide: Large ribosomal subunit protein uL16c
• Protein or peptide: Large ribosomal subunit protein bL17c
• Protein or peptide: Large ribosomal subunit protein uL18c
• Protein or peptide: bL19c
• Protein or peptide: Large ribosomal subunit protein bL20c
• Protein or peptide: bL21c
• Protein or peptide: Large ribosomal subunit protein uL22c
• Protein or peptide: uL23c,Large ribosomal subunit protein uL23c
• Protein or peptide: KOW domain-containing protein
• Protein or peptide: bL27c
• Protein or peptide: Large ribosomal subunit protein bL28c
• Protein or peptide: uL29c
• Protein or peptide: bL32c
• Protein or peptide: 50S ribosomal protein L31
• Protein or peptide: Ribosomal protein S1 homologue
• Protein or peptide: Small ribosomal subunit protein uS4c
• Protein or peptide: bS6c
• Protein or peptide: Small ribosomal subunit protein uS7c
• Protein or peptide: Small ribosomal subunit protein uS8c
• Protein or peptide: Small ribosomal subunit protein uS10 domain-containing protein
• Protein or peptide: Small ribosomal subunit protein uS11c
• Protein or peptide: Small ribosomal subunit protein uS12c
• Protein or peptide: uS13c
• Protein or peptide: Small ribosomal subunit protein uS14c
• Protein or peptide: 30S ribosomal protein S15
• Protein or peptide: 30S ribosomal protein S16, chloroplastic
• Protein or peptide: Small ribosomal subunit protein uS17c
• Protein or peptide: Small ribosomal subunit protein bS18c
• Protein or peptide: Small ribosomal subunit protein uS19c
• Protein or peptide: bS20c
• Protein or peptide: bS21c
• Protein or peptide: 30S ribosomal protein 3, chloroplastic
• Protein or peptide: cS26/PSRP8
• Protein or peptide: Plastid-specific ribosomal protein-7
• Protein or peptide: Small ribosomal subunit protein uS2c
• Protein or peptide: Small ribosomal subunit protein uS3c
• Protein or peptide: uS5c
• Protein or peptide: Small ribosomal subunit protein uS9c
• RNA: 5S rRNA
• RNA: 3S rRNA
• RNA: 23S rRNA
• RNA: 7S RNA
• RNA: P-site tRNA
• RNA: Nascent peptide
• RNA: 16S rRNA
• RNA: mRNA
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM ION
• Ligand: CHLORAMPHENICOL

Supramolecule #1: Chloroplast ribosome

• Supramolecule: Name: Chloroplast ribosome / type: complex / ID: 1 / Parent: 0
• Source (natural): Organism: Chlamydomonas reinhardtii (plant) / Strain: mat3-4

Macromolecule #1: cL38/PSRP6

• Macromolecule: Name: cL38/PSRP6 / type: protein_or_peptide / ID: 1 ; Details: Proper UNIPROT ID is A8IMN3 that has been sent to UniParc.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 7.21533 KDa

Sequence

String:

MFALSAVSSR NVACQASQPR HKGAKHHARS RPIKYNRADK NHGPAKYEPL PTPPPALIVV SKAESK

UniProtKB: Uncharacterized protein

Macromolecule #4: bL33c

• Macromolecule: Name: bL33c / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 11.43135 KDa

Sequence

String:

MQSVLRSSAS ALTNPMMGNK MSYRVSAAPV PTTTAFREVT TMAKKKGVRL IVTIECTESK GEGATPSRYC TQKNRKNTPE RLELMKYNP NLRRYTLHKE VK

UniProtKB: Uncharacterized protein

Macromolecule #5: bL34c

• Macromolecule: Name: bL34c / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.987529 KDa

Sequence

String:

MASMLMSRVA AAPVKAVACM RSAMTTRAIA PITARPATSS FMGGSFAGSS MTRVAPARGG LVVMANAKKS IACTKEGTNR KRRRTSGFK ARMATKNGRK VIKARRAKGR HSLCPASEGK SGGKK

UniProtKB: Uncharacterized protein

Macromolecule #6: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.106231 KDa

Sequence

String:

MASLCMRSSL APKVASRSSA FLASPVAPVR SVAPVKAAPT TVVVEAKLKT RKSAAKRFKV TGSGKVTARH AGKQHFNEKM TRDHIRDSS KMFVLSPANI YNATKCLPNS GVGGK

UniProtKB: 50S ribosomal protein L35

Macromolecule #7: Large ribosomal subunit protein bL36c

• Macromolecule: Name: Large ribosomal subunit protein bL36c / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 4.273327 KDa

Sequence

String:

MKVRASVKKM CDNCRVIKRK GKVMVICSNA KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36c

Macromolecule #8: Large ribosomal subunit protein uL2c

• Macromolecule: Name: Large ribosomal subunit protein uL2c / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 30.95577 KDa

Sequence

String:

MGIRFLQAYT PGTRNRSVSD FSELTDKNST PEKALTVSLH RAKGRNNRGI ITCRHRGGGH KRLYRQIDFR RDKIGVTAKV VRIEYDPNR NARIALLRYE DGEKRYIIHP RGLNIGDIIQ SDLNAPILIG NSLPLRNIPL GAEVHNVEFQ PGSGGQLARS A GAMVEILA KEGNFVTIRL PSKEIRLVSK NCWATVGQVG NIEAYNLTIG KAGRTRWLGK RPTVRGSVMN PVDHPHGGGE GR APIGRSR PVTPWGRPAL GQLTRKPKKY SNTLIVKKRK K

UniProtKB: Large ribosomal subunit protein uL2c

Macromolecule #9: Large ribosomal subunit protein uL3c

• Macromolecule: Name: Large ribosomal subunit protein uL3c / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 27.878426 KDa

Sequence

String:

MALQQTRAFS GRMSLKSAAA PVRVSRASRA RTVLVEARQF RKAMGVLGTK AGMMSYFTED GLCVPATVIA LEEGNVVTQV KTQDTDGYN AVQIGYKATA EKRVTKPELG HLKKAGVPPM RHLVEFKLKD RAAVEAYQPG QALDVAALLK EGEPVDIAGI T VGKGFQGT IKRWHHKRGA MSHGSKSHRE HGSIGSATTP SRVFPGLKMA GQMGNVRMTV KNQSLLKVDT ERHALVVKGS VP GKVGNVV EITPAKLVGV NW

UniProtKB: Large ribosomal subunit protein uL3c

Macromolecule #10: Large ribosomal subunit protein uL4c

• Macromolecule: Name: Large ribosomal subunit protein uL4c / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 25.659525 KDa

Sequence

String:

MQTMRVAFRP AATSRSTVVT RASAVAAPAS IPYKAADGSS KGTQQLALKV AEDSAKGLVH RYLVMVQQNA RQGTASTLTR SEVRGGGKK PYAQKGTGNA RRGSSVSPLF PGGGVTFGPK PKDWSISMNK KERRLALATA LQSATADMIV VESLAGKLQD T KTKSMVAL LEKLGANAME RKVLLITKEE RPDVTLAGRN IAKLTMNTAS AISVFDVLNA DHIIIEDEAL AHVQSFYGAA AP ASA

UniProtKB: Large ribosomal subunit protein uL4c

Macromolecule #11: Large ribosomal subunit protein uL5c

• Macromolecule: Name: Large ribosomal subunit protein uL5c / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 20.267668 KDa

Sequence

String:

MTQRLKNLYT KTIVPKLTTN FNYSNMHEVP KIEKIVINRG IGDASQNQKI VESSLKELAM IAGQKGVVTR SKKAIAGFKL RQQMPVGVT VTLRGDRMYG FLDRLIHLAL PRVRDFQGIS SKSFDKKGNY SLGLEEQLMF PEIEYDKIDQ VRGMDISIVT T AKTQEEGL ALLKEFGLPF K

UniProtKB: Large ribosomal subunit protein uL5c

Macromolecule #12: Plastid ribosomal protein L6

• Macromolecule: Name: Plastid ribosomal protein L6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 22.275268 KDa

Sequence

String:

MLTTRVKPFR AAAVSRSSRL CVVAKDSRIG RAPITVPKGV TVTLEGQLVR VKGPNGTLEQ TLSPLVKIEQ ADGKLKLFKL ADDRVAMSQ HGLNRSLVNN LVVGVSTGFE KRMEMVGTGY RAAVAGKDLT LNVGYSKPRV LAIPEGLKVV VEKNTTLVIS G ADKVKVGD FCATIRRQRP PEPYKGKGIR YAGEVIKLKE GKGAGGKKK

UniProtKB: Large ribosomal subunit protein uL6 alpha-beta domain-containing protein

Macromolecule #13: Large ribosomal subunit protein bL9c

• Macromolecule: Name: Large ribosomal subunit protein bL9c / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 22.043705 KDa

Sequence

String:

MRGAPVSRAA FKAAPVRAAS SRTALVVEAN KKVAKKTKII LISDIPNVGK EGEIKTVPVG YWRNFLLPNG MAKIASEGIL NQIRAKKED EIRKKLEEKA QAQAFANALT TIGKFLLKKK TGEKDQIYGS VQVQEIADAI YQQTGRNVSD CEITVPEIKS V GTYECTIR LHPEVVGTFS VVIQKEKNQI KVTTTEPKKA KK

UniProtKB: Large ribosomal subunit protein bL9c

Macromolecule #14: uL10c

• Macromolecule: Name: uL10c / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 25.94116 KDa

Sequence

String:

MQSSTMTLRG QRSQFGAKAF TSARVQRASL QVTNAITRQK KEQVVEVLKG KLEKSTVVFG LRFKGLDVPT VQKFRKILPA NTSVYVTKN SLMKVAVSQT KGWEALADKG CTGENAWVFV NEEEIAETVK AYFKFEEDLF VEAKKQAAKN VEVKRPTEVS C AVMSGQYL SPAELKRCEN LPTKDQLYAT IARLAKQPAQ KLAVGIKAVP NKLAIAIKKV SELDDDKTKT VASVAKA

UniProtKB: Uncharacterized protein

Macromolecule #15: Large ribosomal subunit protein uL11c

• Macromolecule: Name: Large ribosomal subunit protein uL11c / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.562955 KDa

Sequence

String:

MAAVMPRSGV CLPTRSARLA FAPARVATAN RKVTTMAAKV TGKIKLALPA GKANPAPPVG PALGSKGVNI MAFCKEYNAQ TSKMIGDII PVEITVYEDR SFTFVLKTSP ASILLKKAAG IDRGASKPNK ENVGQVTRKQ VAEIAAKKLV DTNATKVESV V RMIEGTAK NMGIKIVD

UniProtKB: Large ribosomal subunit protein uL11c

Macromolecule #16: uL13c

• Macromolecule: Name: uL13c / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 24.590453 KDa

Sequence

String:

MALTASKLSG RTAFTTERRV AVRAAFQSTR VAARVAPVSR VQSVVCKASA TEFKGDLLNK SYYPTSADAS NVNKRWYVID AEGQTLGRL ASLAALYIRG KHLPTYTPSM DMGAYVIVIN ADKVTVSGKK PAAKTYFRHV TGRPGSWTIE SFNELQARLP E RIIERAVK GMLPKGALGR DIKLHLKVYK GTKHDHEAQQ PQDITKEISI KPKNGPGAAV LKAAKAQ

UniProtKB: Uncharacterized protein

Macromolecule #17: Large ribosomal subunit protein uL14c

• Macromolecule: Name: Large ribosomal subunit protein uL14c / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 13.466755 KDa

Sequence

String:

MIKPLSYLNV ADNSGARELM CIRALGGSYR ESANIGDVII AVVKDALPNM PVKRSDIVRA VIVRTRKGIR RENGMAIRFD DNAAVIINK EGNPRGTRVF GPIARELRDK NFTKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14c

Macromolecule #18: uL15c

• Macromolecule: Name: uL15c / type: protein_or_peptide / ID: 18 ; Details: Proper UNIPROT ID is A8JAL6 that has been sent to UniParc.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 25.570533 KDa

Sequence

String:

MQVASRASVR PFTAARQPRS LVVVNASSDA RLRLHNLSPE PGSRRDEKRK GRGYAAGQGG TCGFGNRGQK ARSGPSVRPG FEGGQTPLY RRLPKLRGIA GGMSAGLPDF VVVNLDDLEK HFNAGEDVTL AAVKEKVKSI SGRDASLPLK VLGTGSLSKS L NVHATAFS ESAKAAIEAA GGKAEVLAAK TKWTKKVADM AAKGMDYEKE KLKKRIDNLK AKGMFEERVP KAKPAAAAAG KK K

Macromolecule #19: Large ribosomal subunit protein uL16c

• Macromolecule: Name: Large ribosomal subunit protein uL16c / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 15.500425 KDa

Sequence

String:

MLSPKRTKFR KPHRGHLRGK ATRGNKIVFG DFALQAQEPC WITSRQIEAG RRVLTRYVRR GGKLWIRIFP DKAVTMRPAG TRMGSGKGA PDYWVAVVHP GKILYEMQGV SETIARQAMR IAAYKMPVKT KFLTKTV

UniProtKB: Large ribosomal subunit protein uL16c

Macromolecule #20: Large ribosomal subunit protein bL17c

• Macromolecule: Name: Large ribosomal subunit protein bL17c / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 19.188451 KDa

Sequence

String:

MATLQMARSG VFTSRASAPR AVVRAVVPVA PRSAQTSSFL GEATLVAPSS GMKWTMMRHG NRVKHLGRPA DQRKALIRSL VTEVIRHGA IRTTKVKAMV IRKYVDHMIT LAKDGSLHAR RQAMAFLYDK DLVGNLFENA PERYAERPGG YTRVKTEPVL R RGDATEMA VIELV

UniProtKB: Large ribosomal subunit protein bL17c

Macromolecule #21: Large ribosomal subunit protein uL18c

• Macromolecule: Name: Large ribosomal subunit protein uL18c / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 15.818282 KDa

Sequence

String:

MQSTMFKATL APTRPVSRAF TPVVCKAANK KASTQARHAR LRKKLSGTTE RPRMAVYKSN EHIYVQVIDD SKGHTLVSAS TVMKDLKES LESGANINAA TAVGKKVAEL CKAHNIEKVC FDRGGFQYHG RIQAVAEAAR EGGLVF

UniProtKB: Large ribosomal subunit protein uL18c

Macromolecule #22: bL19c

• Macromolecule: Name: bL19c / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 16.626418 KDa

Sequence

String:

MLASRSAVRP FVAGRRPTVA CSAKLSLHTL RQDMARSQLK TNLPAVVVGD NVKVGLAVQE GNGKTRTQTL DGVIIAAHGA DSSRTLTFR RVFQGVGVEL VMPVHSPAVQ QIEFVRRGRV RRAKLFYLRE RVGKSARLKE VVGVRAADVA AGKQ

UniProtKB: Uncharacterized protein

Macromolecule #23: Large ribosomal subunit protein bL20c

• Macromolecule: Name: Large ribosomal subunit protein bL20c / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 13.578073 KDa

Sequence

String:

MTRVKRGNVS RKRHKKILNM SKGFRGAAST LFRTANQQNM KALRYSYRNR RQKKRDFRRM WITRVNSAVR RYGLNYSEFM NYLKTHKIQ LNRKVIAQLS ICDPEAFMQL LLF

UniProtKB: Large ribosomal subunit protein bL20c

Macromolecule #24: bL21c

• Macromolecule: Name: bL21c / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 19.516861 KDa

Sequence

String:

MALANMLSRK AGFATSRTSA PFVASRVSPV AAKARVSSAA PSFSRQQQSI VCQAAATAEV AEKKVHPSPL TIAAPATYAI VEIGGTQMF VEPGKWYTCN RLKVDVGSKI KFGRVLALKH EGKLTVGKPY LENVAVEAEV IEELRGPKVI VYKMRPKKHY R KKQGHRQD LSKFMVTSIS A

UniProtKB: Uncharacterized protein

Macromolecule #25: Large ribosomal subunit protein uL22c

• Macromolecule: Name: Large ribosomal subunit protein uL22c / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.959348 KDa

Sequence

String:

MALRACSSSL VARSAARSVQ PFRPAAVRPA RIAPVARKTA TVVCSAATDA PAAPVAEAVV GEVKSGVAHL KFQRGSADKI RRVLDVIRG RSYEEALVLC EYMPYRACEK IIKCLMSAAA NAKHNKGALK TKLVVSECFA DEGPVLKRAR PRAQGRANLI M KPTFHMTI RVEERAQ

UniProtKB: Large ribosomal subunit protein uL22c

Macromolecule #26: uL23c,Large ribosomal subunit protein uL23c

• Macromolecule: Name: uL23c,Large ribosomal subunit protein uL23c / type: protein_or_peptide / ID: 26 ; Details: N-ter extension not present in the Uniprot sequence,N-ter extension not present in the Uniprot sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.565778 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) MLD LVKYPVITQK TYIALFKDRQ YTFDVDLRLT KPQIKKVFET LFNVDVISVN THIPPRQKIR VGLAQGYRPR YKRAIIT LK EGQSINYSLK NDN

UniProtKB: Large ribosomal subunit protein uL23c

Macromolecule #27: KOW domain-containing protein

• Macromolecule: Name: KOW domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.307617 KDa

Sequence

String:

MATMLSARTS AFSGATLGRS VAPRAPISSR TTVCAPVAAY GDLPKIGGGR KWEHLELGPG GKALRVPMHV KKGDTVQIIA GKDKGKVGT IIKVLAKKGK VVVEGVNQGQ KHLKPKMENE TGQIKTLEFP IHHSNVMLYS KEKGVRSRVG HKVLETGKKV R YLVKTGEI ID

UniProtKB: KOW domain-containing protein

Macromolecule #28: bL27c

• Macromolecule: Name: bL27c / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 17.20876 KDa

Sequence

String:

MLASTIQSSV LRSSVRAAPA PKRALVIQNA HKKGSGSTKN GRDSNAKRRG VKVYGGQPVK AGGIIVRQCG STWNAGDNCM LGRDFTVFS TVEGVVVFDK KRVKPEIHVY PADHPKAVAA LAKTHTKQPA EGQQSRAERR KATYTSRKAT LSVAQVAAPT T P

UniProtKB: Uncharacterized protein

Macromolecule #29: Large ribosomal subunit protein bL28c

• Macromolecule: Name: Large ribosomal subunit protein bL28c / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 21.945557 KDa

Sequence

String:

MSLSMRSSIV GQSLAFKPAT AQVRVARVLT VDAKKVCDLT GKKRNKANSV CFSNKKSRKW QEPNLQNKKV FWEEGQRWVK LRICTKAIK TLENKGIATM AAEAGIDLWK LPFEDARPQR KAYLAENAGK VPVGSNPRAM KNAEKLASSK KQPKYPVYEE G GRIVFIRP GMEDLIFGKK EQQAAPEDQE LKITLSE

UniProtKB: Large ribosomal subunit protein bL28c

Macromolecule #30: uL29c

• Macromolecule: Name: uL29c / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.965354 KDa

Sequence

String:

MQVARAFTAV RPARSVAAPA QQRVSVVAMA KPTKLADFTG LSNEELVNKS NLLKRELAQT KWLQRTRGVG ELKPGENQPQ PDPEKVPKG HLNKHIRRQI AQCLTLLRQR QAAEGVDRKE ARRIEKRAAL ANGTM

UniProtKB: Uncharacterized protein

Macromolecule #31: bL32c

• Macromolecule: Name: bL32c / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 10.639328 KDa

Sequence

String:

MLALSSSRSA AFSATVRVQP ARQVALAPSR SSLIVEAAVP KKKTSKSKTA IRKAAWKKEV LPYVEQALFK AKLALKEGSR DSADKDMVV STQTEEKSE

UniProtKB: Uncharacterized protein

Macromolecule #32: 50S ribosomal protein L31

• Macromolecule: Name: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.777946 KDa

Sequence

String:

MATLTSSRVT AFSGSRLGNV RIGQARRGVT AARMAKEGIH PQWFEEAKVI CNGVEVMTVG GTKATYNVDI YSGNHPFYQG NRTTMVLDD GQLNKFKKRF AELEELAVVP ILQAGKAEDP SAKEKPAAAA NKKGKKK

UniProtKB: 50S ribosomal protein L31

Macromolecule #37: Ribosomal protein S1 homologue

• Macromolecule: Name: Ribosomal protein S1 homologue / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 48.345176 KDa

Sequence

String:

MRLSARTLAQ RASLPARSTG VVSRRSLVAP APARKANLAT RVAAIEEQQT SFQGDEQPVE AALDPASILD VDPAAVEEMD LHELDQAQE DLLKWMMLDE ATQEEDLDEM VDYDDFGDEE YADMYEEVEE MLEAVDYEFK VGDKVSGTVI EVDDDGAYVE I GAKAIAFC PVTECSFARL KTPLEALRPG MKRDFMVVED EEEYGQVIVS LAAMEATTFW QRIRQLQEED ITVTVSVESV NK GGMLVKF GIYDGFIPVS QFGPSITPDN MESLVGFELQ VKFLEVDEEA ERLVFSHKRA SSTVSNDIQG FKVGDVVAGV VQS VKPYGA FIDLGGVTGL LHVSQISHDR VLVLDKILQE GDKIKVMILS QDRERGRVTL STKKLEPTPG DMLRDPQLVY EKAE EMAEQ FRKRVEQAAQ SAAEPQPDTL GAAPEGQPFV Y

UniProtKB: Ribosomal protein S1 homologue

Macromolecule #38: Small ribosomal subunit protein uS4c

• Macromolecule: Name: Small ribosomal subunit protein uS4c / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 30.082121 KDa

Sequence

String:

MSRYLGPRLR VIRRIGKLRG FTRKKPFRRV FKGFGGFKGK VIPPGQHGLT KLLKTRPYDS SESDYLIRLK VKQRLRFNYG ITERQLVNY VRKAKKIKES TGQVLLQFLE MRLDNIVFRL NMAPTIPAAR QLISHGHIRV NNKKVNIPSY MCKPKDVISV A MKQRSLQL VNKNLQEYYR RMRFYKKRLE KTLPFILLKI KPLGLTSVTA AVELITKGNV RVNNKSVKTP NYICRPRDTV SL RTKQGIK KVFLKNYLKG

UniProtKB: Small ribosomal subunit protein uS4c

Macromolecule #39: bS6c

• Macromolecule: Name: bS6c / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 19.096979 KDa

Sequence

String:

MATMQKSSVA VRSSSSANLV AARPIAQRLC FQPVVRQSAQ QQRSSVGRAM PKVVAAAVAA DAAAETVKLP AGYHWYETMI ILKPTLSDE ERDRELAKFE AYLNKEECFN INALVRGRSK LAYPIKKEWE GIYVLYSYLA RRQTARAVQQ LLSNPEAGAE D TVLRHITL CKQ

UniProtKB: Uncharacterized protein

Macromolecule #40: Small ribosomal subunit protein uS7c

• Macromolecule: Name: Small ribosomal subunit protein uS7c / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 19.163799 KDa

Sequence

String:

MPRRPINKKR TLLPDPVYNS VSVHMLVNRV LKSGKKSVAY RIVYNALKEI GDVTQKNPVE VFEKALDNVT PRVEVKPRRR AGAIQMVPR VLRLGDRARA NSLRWIMEAC DKRSGQPMVT KLKSEILDAY KKTGFAIRKK EELHKIAIAN AMYAKKPQVI I NAINLLVD

UniProtKB: Small ribosomal subunit protein uS7c

Macromolecule #41: Small ribosomal subunit protein uS8c

• Macromolecule: Name: Small ribosomal subunit protein uS8c / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 15.916647 KDa

Sequence

String:

MKSHGLINDS IGDMLTRIRN ACLAKKSSVS IPFTRLNQNI AQILEQEGFI QTYQVSLDSQ DLTIRLKYRS KKIYRGKKKE SCITNLKRI SKPGLRIYSN HKDILRILGG TGIVIVSTPE GLMTDREARL RGIGGELLCS VW

UniProtKB: Small ribosomal subunit protein uS8c

Macromolecule #42: Small ribosomal subunit protein uS10 domain-containing protein

• Macromolecule: Name: Small ribosomal subunit protein uS10 domain-containing protein; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.736729 KDa

Sequence

String:

MAALTCRVRA FTTSRPQAAR PQVGARPAVR RPVSIVRAVA ESAVDVESYV DELDVPMAES TPLAAAGEKV RLRIRMRSYE KQLLEDCVA QIQAVAEATG AVFKGPVMLP TKRRIYCVLR SPHVNSNSRE HFEIRSHHRL VDLKNLSAET VSMMMQWVPP S GVEVECSI A

UniProtKB: Small ribosomal subunit protein uS10 domain-containing protein

Macromolecule #43: Small ribosomal subunit protein uS11c

• Macromolecule: Name: Small ribosomal subunit protein uS11c / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.260867 KDa

Sequence

String:

MAKQTRKLTT NKTKKKIFRG VVHIQAGHHN TIVTITNIRG EVLCWSSAGA CGFRGKRKST SFAAKKAAET VARKSRDFTM KAAKILVTG PGQGRESAIR EIFKAGIKVS VIREKTGIPH NGCRPPKKRS V

UniProtKB: Small ribosomal subunit protein uS11c

Macromolecule #44: Small ribosomal subunit protein uS12c

• Macromolecule: Name: Small ribosomal subunit protein uS12c / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.637429 KDa

Sequence

String:

MPTIQQLIRS ARKKITKKTK SPALKSCPQR RGICLRVYTV TPKKPNSALR KVARVRLTTG FEVTAYIPGV GHNLQEHAVV LVRGGRVKD LPGVRYHIVR GSLDTAGVKN RVQSRSKYGV KMGSKTAAKT AGKK

UniProtKB: Small ribosomal subunit protein uS12c

Macromolecule #45: uS13c

• Macromolecule: Name: uS13c / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.561682 KDa

Sequence

String:

MQTLSACRCV AGARPFTAVR PLPAARPMQA AAPRRAPLHI MNLRVNNVEI PNSKRIEVSL QYIFGIGETT AQTILRDTGV ENKKTYELN EEEINKLRSE VEKYTTEADL RRVVAQNIKR LKDIGCYRGR RHIMGLPVRG QRTKTNARTR KGKAKTVANK K KATK

UniProtKB: Uncharacterized protein

Macromolecule #46: Small ribosomal subunit protein uS14c

• Macromolecule: Name: Small ribosomal subunit protein uS14c / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 11.776143 KDa

Sequence

String:

MAKKSMIQRE LKRQKLVMKY ATKRAALKEQ IKQTTFLKEK LSLHRKLQQL PRNSSAVRLH NRCMITGRPK GYFRDFGLSR HVLREMAHQ GLLPGVCKSS W

UniProtKB: Small ribosomal subunit protein uS14c

Macromolecule #47: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 15.817261 KDa

Sequence

String:

MQALQCKSGV FGKAAVARPV RVAAPSRARL AVCASYKNQE NINLEKVPAF KRHDADVGSS EVQVARLSAR IMQISAHLAQ NKKDFAARR GLEAILSQRK SLLQYMYKTD RDMYDRMVSE YKIRSVIVGD TRGAARKSEE RS

UniProtKB: 30S ribosomal protein S15

Macromolecule #48: 30S ribosomal protein S16, chloroplastic

• Macromolecule: Name: 30S ribosomal protein S16, chloroplastic / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.474052 KDa

Sequence

String:

MQALSAKRSL GSIQPFTSSR VAVARPARVA TVIENRVALR FQRFGRKKLP FYRLVAIDSK KRRDGEPLEY LGWYDPLKKE TNLNAPAIK EWLAKGALPS ETVENLLRKA YVMEPKVAKV VVPKDAVKV

UniProtKB: 30S ribosomal protein S16, chloroplastic

Macromolecule #49: Small ribosomal subunit protein uS17c

• Macromolecule: Name: Small ribosomal subunit protein uS17c / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 11.767698 KDa

Sequence

String:

MQSATMRTSA FRPAAVSRRS AVTVRAVQEV KGVVVSTKMN KTVVVEAERL ATDVTYQKRK KVTKRYMAHD ESGALRVGDF VRLDGTRPL SKTKRFSVTE VLRKAD

UniProtKB: Small ribosomal subunit protein uS17c

Macromolecule #50: Small ribosomal subunit protein bS18c

• Macromolecule: Name: Small ribosomal subunit protein bS18c / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 16.315782 KDa

Sequence

String:

MRGLKSNKPK PRIQKVSVRK FRRKVLSLSQ ILSRLRQKRN QKIEQQKRNK PIKPLIPPKT LVIVLKEKPE KTLYNRRYID YKHSGLLQR YIGLGGKILP RRQTRLTAKQ QRFVAKTIKT ARVMGLLPFV SKERSFFK

UniProtKB: Small ribosomal subunit protein bS18c

Macromolecule #51: Small ribosomal subunit protein uS19c

• Macromolecule: Name: Small ribosomal subunit protein uS19c / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 10.463467 KDa

Sequence

String:

MSRSLKKGPF VADHLLKKIE KLNAKGKKVV IKTWSRSSMI VPPMIGHTIG VYNGREHIPV FVSDQMVGHR LGEFSPTRTY RGHAKKDKK AKR

UniProtKB: Small ribosomal subunit protein uS19c

Macromolecule #52: bS20c

• Macromolecule: Name: bS20c / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.285582 KDa

Sequence

String:

MQSTMLRAAS AVRAFQPAKP TFAPVSRPAV ERGALVVMAA EGKDAQKKKM PSPVKRALLS EERRVYNKSH KSACATRVKK VVKLAETLV AAPSKTEEEV KNLEKLISEA YTEIDKAVVK GILHENTAAR KKARCARWKK TVLMAAGLYK PAADSPDFAR Y QKLVKA

UniProtKB: Uncharacterized protein

Macromolecule #53: bS21c

• Macromolecule: Name: bS21c / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 20.793336 KDa

Sequence

String:

MLSTVTRQAL AAKPFGTVQP RVAPRLAKCQ MARKDSYMVE VEVAEDEPED VAVRRYMKLV MQSRVVEKLR ARRTKETKIE EYKRRFRER VEMRKAGIVD PTYDELYSAE LDPKPFEDFF QLRRDEDPEG AYDGAGAGSD DIFGSFAGGT YTDTTRWGSG Y PGGGYMNT SNSFGAQGGY MGGYMQ

UniProtKB: Uncharacterized protein

Macromolecule #54: 30S ribosomal protein 3, chloroplastic

• Macromolecule: Name: 30S ribosomal protein 3, chloroplastic / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 33.397238 KDa

Sequence

String:

MMLVRQVPCL AQRASARSAV RSRPAPFTPA RRVFARAQAD AALLEDDEVA YEVTNEDFEL DENDPDLLLG LEAVELMGSD VDAMTAAAV AAEMESAEDK EEAARAVEDV MASKLAAGEL SEAELSKVAA AVGDLEGAEL DDFVPALASD RYAEAAVSSD D KALMEPLK LSKGELRNLL PQDWDQINVD FFSNKKDENI PLPEYRLNVL WTDKNLAVAI DQVYSRGQVS PLTEYYFWPR QD AWEELRI NLESRPWISE RDRIILLNRL TQLINFWQDE EVKHTVEEAR AEFPDCAFAI A

UniProtKB: 30S ribosomal protein 3, chloroplastic

Macromolecule #55: cS26/PSRP8

• Macromolecule: Name: cS26/PSRP8 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.985022 KDa

Sequence

String:

MLATPRASAR PFTSARPAAK APICPRRVTV RAAEEAVEAA PEAFEESMES GPRGRRSSVE KGMELKHFLP LPKPAHLVFI KPTVAVVAG TASRSSVGVK MSAGDQQLER YVVEYFARSV K

UniProtKB: Uncharacterized protein

Macromolecule #56: Plastid-specific ribosomal protein-7

• Macromolecule: Name: Plastid-specific ribosomal protein-7 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 60.136863 KDa

Sequence

String:

MLRELGRTAT VKAHGRSVLR PVRGPAGRRQ VAFTGVRPSV RVFAEAPAAE QAAKAIKLED VKEGSEYEGT VTTVEEFGAF VNFGANTNG LVHISKLASG FTKNAKDVVQ PGQKVTVKVL SVDAEKKRVS LELKSAVAAE ASAEESDDII TEPDREGADA T DDDEDVEV ELEDGQVEVR ADLPGFEDIP FVMEEADMDA EMSEAAIAAL EADLDGAEIR YELEAPAYME EVTGKVARIE DY GVFLEFE WNGKTLTGLL AKDEMKVPSS ALSAEAQAAL RAEWADTGFE MPAFVELPDD ELDVKKYYQP GESVPAFVLE SSL VDGRGI SLTHFTDKEV SAEAVAAYEE LEDDEDEELD KMMADAAGLE DEVLAFDPEA LMEEDEGEEA GAAADAGDDA EYEG VSADG LEGANGNYAL GATRSGLIKG KNGYQVAPMG LPSRPLNDAV TSSGLAILGT SEVDFDGDEV QLVDYWTSEA FDNIP KDVL KKLGLKMSYT EAGEAEFEER ADFEATDVPF YLYGGDVESR AKEFVADLLS DDVDEAELPA RAGRAPIVLA AAVQSQ

UniProtKB: Plastid-specific ribosomal protein-7

Macromolecule #58: Small ribosomal subunit protein uS2c

• Macromolecule: Name: Small ribosomal subunit protein uS2c / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 102.698477 KDa

Sequence

String:

MLNKKPPYLI LSLIMIKTQK SKQTAVRKLI PGNVISLKIT AMGANNVGIN EFTFGIPVLV PNAKLGETVQ AKVLKILASK KIAIAKLIK VVTKTNATET NNLAALTPGA TLDVTITKLG PNSTGIADLG NNYNNVNKLI VKKSAITIGE KVTVLVTRVK N GYAFGVAT VSTQRLNQVS TSLTQNSFKG TKFTVVLPKN AKRYLKHLVF KVTTATAQNL SVNGFEQTLF KKGAVGLDTR IN NQNGSAG NLTTVPTNTI LFVKPNLGAK LGDKVQIQII KFASIENGTL TYNVAIAKII KLNPLSTPQK KAFVRSSLRQ MLK SGMHYG EKAIKCNARM KNYVWTRKKG TDTKVEARPL IKKGRNLINL LKTRRCLTKA LAQLTKYAAK GKTFLFVGTK KAAS GLVAR AALFSKKAFF VNTRWLGGML TNWKTILKSI SKIRPILKEK QMIIKDILEK RQTIKARLIQ KALLLRKKSK LMLKK GRLL IQMLKQNNSN STSGKQAVRF LFTEKTNLLN TKRKEFVSKG ILLLEKRQQL VVKRQELITQ SQTLKSKAIQ LTNTYR NLL NNLICSRKKL RELKALLLVS HELYLFKQQA KQDNQNLYMV SYNKFKTLNS DYILSNPPKE ILNKMVSIIK GQGLVIK NN NLNLKTANNA KTLILSQLLS KFSLFVPTIK TSINNLQNYI STQKTALNKV LALLNVVKTK MNVYVTLKTK LVAELRQI K QTLQTERNII RVLRRKLKQI AAQKRFIKFL PKLRYLPTPV TKIEQTARFL VKKFVDPKMK YPMDSIYDKK LSRQSKKVA ASRKKKWQRL EKYLGGISNM TKIKEKQIAN NVAIIIGQQE EMNAVRECQK LGIKMFHIVD TNCNPGLADH FIPANDDARN SIKFILGKF LTRIRLAHKI KVKFKKTSLK K

UniProtKB: Small ribosomal subunit protein uS2c

Macromolecule #59: Small ribosomal subunit protein uS3c

• Macromolecule: Name: Small ribosomal subunit protein uS3c / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 82.015938 KDa

Sequence

String:

MGQKVHPLGF RVGITKKHQS QWFARFQKYA YSQSVFEDHM LRTTLVNLFS NLEKESALAT KQSKNRGATQ PKAPKITQIK IERGLIPYE IGIQIHSNDC LSITKAIDNI KVSKDLVTNL QKTRKYLFKA GTQLKNASMQ VSDNLNVAEG ENSTMLKTKT S ASGTSVLK KRTFKIQTQK PTKGKFVFKG KRKQKKKLSK AVFMRLKNIK RRFKKRQTIK KRYLNIISKG LLIRKKGNLI IR NVKIKRK NKTARLTSRK SQPTLRSGSN LRDNLAPVKS KMQRFNNRMS KKFANLFLTK LNKQFLVRLK AIMKFWHNQN VTK APLGYN KKWSLAKSYA LINNLKAKYL AKDILSLGSL RVQKLRKLIS ILEKKSLVKM ETLRKDFITF GTLSKTRAFG YYQM ITFLK QLKELVTKIK KQTIANVTTK QELCCYNKLA LNKTKIQNLI RAKSKQTKSI TQKVVNNFVK LVDDNQAMAN ESRKI KWIS YLKDLVNKHR TENIFYYLAT IATARKDLNA LKRYTKQHAN FLFGVNVENA KENPNALLQR VTKTLTQYSK NPLVNN DFE NAEGLTKLQT AFLTQIESQR KMYKANLALT PKISIKFFSV KTTNLLEKAS TVADSIVDAL EKRKAFRGVI KKAKEDL ML RSRVTRVKGV KIQVAGRLNG AEIARSEWVR AGRVPLQTLR ANIDYAYRTA NTIYGIIGVK VWIFKGYSKI

UniProtKB: Small ribosomal subunit protein uS3c

Macromolecule #60: uS5c

• Macromolecule: Name: uS5c / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 72.090727 KDa

Sequence

String:

MATIQSGVAR AASRPQPKVA AGSISSLRVK PFTRATRSVL GAAPRQNVVP RAAEPEAVAT EADDFTFSLS DAKKGNEYTA GDVEAALRF YSGEASAVGA TNDEFVENVF GIEDADFFGD LDNNEAYDDE FIAAGIPEAA PKQRQGGKRG GEGEGAEDSD D IAAAKEAE AMKQIEEQMV LEAELQETGL DQEEEGTYKA VGPAVWDWMS DIAAEDDDEE ISAVAAGGAR SMAADVMAAL PS DEEVFAD LRNANLQDVD VETRDTIEFL LEDFDIENEV KAIPDNVEEV FSVPEFAGLG DADVARIDAL LGEDISLPEL DLS GLDFAD IEDDGLEMSE EAVQKYVASL KSATGAELSE EQIKEIFADE PVQLVDVAAE AAVTMDGVDL TEPAIEALAE SELV FNSVE DKLEDVDDVE EFRTELLALR AMPEANLEAP PEEEVEVLDQ YLSASEQFIA AEEARKAQLA EKVIKGELSA DVLEE EDGE YVDLEKELLM PDDMDDLVDA DDFDDGENWQ ERIIELSRVT KVVKGGKLMG FRCTAIIGNG NGLVGVGCQA GREVAT AVK RALVDAKKSV VRVPLVGAGT IPHRVEAKFN AARCVMVPAA DGTGVLAGSS IRSVLELAGV QNVLAKRIGC RSLLNNA RC AVAALEQLRT LQEVSKARGV PMDRLLLPSS K

UniProtKB: Uncharacterized protein

Macromolecule #61: Small ribosomal subunit protein uS9c

• Macromolecule: Name: Small ribosomal subunit protein uS9c / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 21.062168 KDa

Sequence

String:

MAILAKAVGR RKEAVAQVQI KEGNGLFIIN NKSAQEYLHN DFYSLLAVKA PFDVLSTSKK ADMVSPDLSL TNLESTFTNL MQFSGGENA VKFDTIVKVK GGGLMGQTEA IRLGISRALC LLSTNTNPSA NQNNLPNIYI PEGEGEALAI PNASDIRKQL K DKGYLTQD SRVKERRKYG LKKARKASQY HKR

UniProtKB: Small ribosomal subunit protein uS9c

Macromolecule #2: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 38.858949 KDa

Sequence

String:

CCUUGGUGCU CUUUGCUCAG UUGGACCCAC ACCAAUCCAU CCCGAACUUG GUUGUGAAAA AGCUGAGGGG ACUGAAGAAC UUUACGGGU CGCCGUCUGG AAUCUCAGUU CUAGUGCUAG GG

GENBANK: GENBANK: X03271.1

Macromolecule #3: 3S rRNA

• Macromolecule: Name: 3S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 15.149069 KDa

Sequence

String:

UACGAAGCAG CUGAAUCCUG CACCAUAGAU GGUGAAAGUC CAGUAGU

Macromolecule #33: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 33 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 769.308625 KDa

Sequence

String:

CCCCGAGUAG CAUGGGGCAC GUGAAAUCCC GUGUGAAUCA GCGAGGACCA CCUCGUAAGG CUAAAUACUC CUGAGUGACC GAUAGCGAA AUAGUACCAC GAGGGAAAGG UGAAAAGAAC CCCUGUUGGG GAGUGAAAUA GAACAUGAAA CCGUAUGCUG A CAAGCAGU GGGAGCAAGA GCUUGUGACC GCGUGCCUGU UGAAGAAUGA GCCGGCGACU UAUAGGGAGU GGCUGGGUUA AG GAGUAAA AUCCGGAGCC CAAGCGAAAG CGAGUCUGAA UAGGGCGCAA GUCACUUCUU AUGGACCCGA ACCCGGGUGA UCU AUUCAU GGCCAGGAUG AAGCUUGGGU AACACCAAGU GGAGGUCCGA ACCGACCGAU GU(5MU)GAAAAAU CGGCGGAUGA GCUGUGAAU AGGGGAGAAA UUCCAAUCGA ACUCGGAGCU AGCUGGAUCU CCCCGAAAUG CGUUGAGGCG CAGCGGUAAC G AGAACUGU CUUGGGGUAA AGCUACUGUU UCGAUGAGGG GGUCAUCCCG ACUUACCAAC UCGUGGCAAA CUCAGAAUAC AA GACAUGU CCGUAAACCA GUGAGACAGU GGGGGAUAAG CUUCAUUGUC AAGAGGGAAA CAGCCCAGAU CACCAGCUAA GGC CCCUAA AUGGUCACUA AGUGGAAAAG GAUGUGAGAA UGCUGAAACA ACCAGGAGGU UUGCUUAGAA GCAGCCACCC UUCA AAGAG UGCGUAAUAG CUCACUGGUA AAGCGUUCUU GCGCCGAUAA UGGCCGGGAC UAAGUGACCU GCCGAAGCUG UGAUA UAAU UUAUAGGUAG GGGAGCGUUC CGCUCUCGGG UGAAGUUUUC ACGUAAGUGG GGAUGGACGA AGCGGAAGUG AGAAUG UCG GCUUGAGUAA CGAAAACAUU GGUGAGAAUC CAAUGCCCCG AAAACCUAAG GGUUCCUCCA CUAGGUUCGU CCAUGGG GG GUUAGUCAGG ACCUAAGACU AGGCCAAACG GCGUCGUCGA UGGAAAACAG GUUAAUAUUC CUGUACGAGU UAAAUUAU G AUCUGAGGGA CGAAGGAGGC UAAGCUAGAA CUGUUUUUGG AUUGCAGUGG AAGCGUUUAG ACGUUGAGAG AUAGAAAAA AAGCUAUCUU GAGUGGAGAC GUGAUCCCUA UUUGAUCGAG UAGCUAGUGA UGUCAUACUU CCAAGAAAAG CUCAUACAUC UUAUAAUUU AAUGACCUGU ACCUGAAACC GACACAGGUA GGUUGGUAGA GAAUACCAAG GGGCGCGAGA GAACUCUCUC U AAGGAACU CGGCAAACUG GCCCCGUAAC UUCGGAAGAA GGGGCACCCA UCCGUAACAA GGUGGGUCGC AGUGACCAGG CC CAGGCGA CUGUUUACCA AAAACACAGG UCUCCGCAAA GUCGUAAGAC AAUAUAUGGG GGCUGACGCC U(2MG)CCCAGUG CCGGAAGGUU AAGGAAGUUG GUUAUUUCGA AAAAAGCUGA CGACCGAAGC CCCGGUGAAC GGCGGUCGUA ACUAUAACGA UCCUAAGGU AGCGAAA(5MU)UC AUUGUCGAGU AAGUUUCGAC (5MC)UGCACGAAA GGCGUAACGA UCUGGGCGCU GUC UCGGAG AGAGGCUCGG UGAAAUAGAC UUGUCCCGUG (6MZ)AGAUGCGGA CUACCUACAC CUGGACAGAA AGACCCUAUG AAGCUUGAC UGUAUCUUGG AAUUGGGUUU GGAAUCCUAA UGGGGAUCCU UGAAUCAGGA CCCUUGACAG UUUCAGGUGG G CAGUUUAU UUG(OMG)GGCGAA UGCCUCCUAA AAGGUAACGG AGGCGUGCAA AGGUUCCCUC AGUCUGGACG GAAAUCAG A CAUUGAGUGU AAAGGCAAAA GGGAGCUUGA CUGCAAGACC UACAAGUCGA GCAGGGGCGA AAGCCGGCCU UAGUGAUCC GACGGUGCCG CGUGGAAGGG CCGUCGCUCA ACGGAUAAAA GUUACUCUA(2MG) GGA(H2U)AACAGG CUGAUCUUCC CC AAGAGUU CACAUCGACG GGAAGGUUUG GCA(OMC)CUCG(2MA)U GUCGGCUCAU CACAUCCUCG GUCUGUAGUA GGUCC GAAG GGUUGGGC(OMU)G UUCGCCCAUU AAAGUGGUAC GUGAGCUGGG UUCAAAACGU CGUGAGACAG UUUGGUCCAU AU CCGGUGU AGGCGUUAGA GCAUUGAGAG UAGCCUUUCA UAGUACGAGA GGACCUGAAA GGACAUGCCA AUUGUGUACC AGU UCUCAU UCCAAUGGGA AACGCUGGGU AGCUACGCAU GGAUAGAUAA CUGCUGAAAG CAUCUAAGUA GGAAGCUAAA CUCA AGAUG AGUGCUCUCU AAGGCCGCGG CUAGACAAGC CGUUAUAUAG GUAUCAGGUG UACAGUCAGC AAUGGCUUUA GCCGA GAUA UACUAAAGGC CGUUU

Macromolecule #34: 7S RNA

• Macromolecule: Name: 7S RNA / type: rna / ID: 34 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 88.325664 KDa

Sequence

String:

AAGGCGUACG GUGGAGACCU AGGCACUCAG AGACGAAGAA GGGCGCAGAU ACCGGCGAUA CGCUUCGGGG AGCUGGCAAC AAGCUUUGA UCCGAAGAUU CCCGAAUAGG GCAACCUCAU AGAACUACCU AUAUAAUUCA UAGUUAGGUA AGAGGCAACC C AGUGAACU GAAACAUCUA AGUAGCUGGA GGAAAAGAAA GCAAACGCGA UUCCCGUAGU AGCGGCGAGC GAACCGGGAA CA GCCUAAA CCUAUGUAUG UAGGGGUCGU GGGAA

Macromolecule #35: P-site tRNA

• Macromolecule: Name: P-site tRNA / type: rna / ID: 35 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 24.565742 KDa

Sequence

String:

GCGGGG(4SU)GGA GCAGCCUGGU AGCUCGUCGG G(5MC)UAAUAACC CGAAGGUCGU CGG(5MU)(PSU)CAAAU CCGG CCCCC GCAACCA

GENBANK: GENBANK: CP119982.1

Macromolecule #36: Nascent peptide

• Macromolecule: Name: Nascent peptide / type: rna / ID: 36 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 380.567 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #57: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 57 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 476.372562 KDa

Sequence

String:

CCAUGGAGAG UUUGAUCCUG GCUCAGGACG AACGCUGGCG GCAUGCUUAA CACAUGCAAG UCGAACGAGC AAAGAUUUGU GUAGUGGCG AACGGGUGCG UAACGCGUAA GAACCUACCU AUCGGAGGGG GAUAACAUUG GGAAACUGUU GCUAAUACCC C AUACAGCU GAGGAGUGAA AGGUGAAAAA CCGCCGAUAG AGGGGCUUGC GUCUGAUUAG CUAGUUGGUG GGGGUAACGG CC UCCCAAG GCCACGAGCA GUAGCUGGUC UGAGAGGAUG AUCAGCCACA CUGGGACUGA GACACGGCCC AGACUCCUAC GGG AGGCAG CAGUGAGGAA UUUUUCGCAA UGGGCGCAAG CGACGGAGCA AAGUGCCGCG UGCAGGAAGA AGGCCUGUGG GUCG UAAAC UGCUUUUCUC AGAGAAGAAG UUCUGACGGU AUCUGAGGAA UAAGCACCGG CUAACUCUGU GCCAGCAGCC (G7M) CGGUAAUA CAGAGGGUGC AAGCGUUGUC CGCAAUGAUU GGGCGUAAAG CGUCUGUAGG UGGCUCGUAA AGUCUAAUGU CA AAUACCA GGGCUCAACC UUGGACCGGC AUUGGAGUAC UCACGAGCUU GAGUACGGUA GGGGCAGAGG GAAUUCCAUG UGG AGCGGU GAAAUGCGUA GAGAUAUGGA GGAACACCAG UGGCGAAGGC GCUCUGCUGG GCCGAAACUG ACACUGAGAG ACGA AAGCU GGGGGAGCGA AUAGGAUUAG AUACCCUAGU AGUCCCAGCC GUAAACUAUG GAGACUAAGU GCUGCCGCAA GCAGU GCUG UAGCUAACGC GUUAAGUCUC CCGCCUGGGG AGUAUGCUCG CAAGAGUGAA ACUCAAAGGA AUUGACGGGG GACCGC ACA AGCGGUGGAU UAUGUGGAUU AAUUCGAU(2MG)(5MC) AACGCGAAGA ACCUUACCAG GGUUUGACAU GUCAAGAAC CUCUCAGAAA UGGGAGGGUG CCCUAACGGA CACUUGAACA CAGGUGGUGC AUGGCUGUCG UCAGCUCGUG CUGUGAAGUG UAUAGUUAA GUCUCAUAAC GAGCGCAACC CUCGUCUUUA GUUGCCAUUU GGUUCUCUAA AGAGACUGCC AGUGUAAGCU G GAGGAAGG UGAGGAUGAC GUCAAGUCAG CAUGCCCCUU ACAUCCUGGG CUUCACACGU AAUACAAUGG UUGGGACAAU CA GAAGCGA CCUCGUGAGA GCUAGCGGAU CUGUUAAACC CAACCUCAGU UCGGAUUGUA GGCUGCAACU CGCCUACAUG AAG CCGGAA UCGCUAGUAA UCGCCAGUCA GCUAUAUGGC GGUGAAUACG UUCCCGGGUC UUGUACACAC CG(4OC)CCGUCAC ACCAUGGAA GCUGGUUCUG CUCCAAGUCG UUACCCUAAC CUUCGGGAGG GGGGCGCCUA AAGCAGGGCU AGUGACUAGG G UGAAGUCG (UR3)AACAAGGUA GGGCUACU(2MG)G (MA6)(MA6)GGUGGCCC UGGCUCAC

Macromolecule #62: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 62 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 1.884197 KDa

Sequence

String:

AAAAUU

Macromolecule #63: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 63 / Number of copies: 319 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #64: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 64 / Number of copies: 130 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #65: CHLORAMPHENICOL

• Macromolecule: Name: CHLORAMPHENICOL / type: ligand / ID: 65 / Number of copies: 1 / Formula: CLM
• Molecular weight: Theoretical: 323.129 Da

Chemical component information

ChemComp-CLM:
CHLORAMPHENICOL / antibiotic*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 2.5 mg/mL
• Buffer: pH: 7.5
• Grid: Model: Quantifoil R2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 75000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: cryoSPARC (ver. 4.7) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE
• Final reconstruction: Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 2.84 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7) / Number images used: 49141
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.7)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.7)
• Final 3D classification: Software - Name: cryoSPARC (ver. 4.7)