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TitleDirect electron detection yields cryo-EM reconstructions at resolutions beyond 3/4 Nyquist frequency.
Journal, issue, pagesJ Struct Biol, Vol. 177, Issue 3, Page 589-601, Year 2012
Publish dateJan 21, 2012
AuthorsBenjamin E Bammes / Ryan H Rochat / Joanita Jakana / Dong-Hua Chen / Wah Chiu /
PubMed AbstractOne limitation in electron cryo-microscopy (cryo-EM) is the inability to recover high-resolution signal from the image-recording media at the full-resolution limit of the transmission electron ...One limitation in electron cryo-microscopy (cryo-EM) is the inability to recover high-resolution signal from the image-recording media at the full-resolution limit of the transmission electron microscope. Direct electron detection using CMOS-based sensors for digitally recording images has the potential to alleviate this shortcoming. Here, we report a practical performance evaluation of a Direct Detection Device (DDD®) for biological cryo-EM at two different microscope voltages: 200 and 300 kV. Our DDD images of amorphous and graphitized carbon show strong per-pixel contrast with image resolution near the theoretical sampling limit of the data. Single-particle reconstructions of two frozen-hydrated bacteriophages, P22 and ε15, establish that the DDD is capable of recording usable signal for 3D reconstructions at about 4/5 of the Nyquist frequency, which is a vast improvement over the performance of conventional imaging media. We anticipate the unparalleled performance of this digital recording device will dramatically benefit cryo-EM for routine tomographic and single-particle structural determination of biological specimens.
External linksJ Struct Biol / PubMed:22285189 / PubMed Central
MethodsEM (single particle)
Resolution7.9 - 8.5 Å
Structure data

EMDB-5375:
Direct electron detection yields cryo-EM reconstructions at resolutions beyond .75 Nyquist frequency
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-5377:
Direct electron detection yields cryo-EM reconstructions at resolutions beyond .75 Nyquist frequency
Method: EM (single particle) / Resolution: 7.9 Å

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