EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Sub-2 Å cryo-EM structures of transcribing RNA polymerase II reveal critical roles of water molecules in catalysis.
ジャーナル・号・ページ	Mol Cell, Vol. 86, Issue 10, Page 1896-11910.e7, Year 2026
掲載日	2026年5月21日
著者	Qingrong Li / Gangshun Yi / Yue Wu / Sophy Xu / Jenny Chong / Xuhui Huang / Peijun Zhang / Dong Wang /
PubMed 要旨	RNA polymerase II (RNA Pol II) is central to gene expression, but its catalytic mechanism remains elusive due to the absence of high-resolution structural data. The role of water molecules in RNA Pol ...RNA polymerase II (RNA Pol II) is central to gene expression, but its catalytic mechanism remains elusive due to the absence of high-resolution structural data. The role of water molecules in RNA Pol II catalysis is unknown. Here, we present 3 high-resolution cryo-electron microscopy structures of active Saccharomyces cerevisiae RNA Pol II elongation complexes in distinct catalytic states: two pre-catalysis states at 1.96 Å and 2.26 Å resolution and a post-catalysis state at 2.33 Å resolution. Each structure contains over 700-1,350 ordered water molecules, many located at functionally critical positions. Comparative analysis shows that these waters play essential roles in proton-transfer steps during RNA Pol II catalysis, facilitating substrate recognition and trigger-loop folding during nucleotide addition. Strikingly, these waters are conserved between prokaryotic and eukaryotic transcription machineries (see Mueller and Darst). These findings provide unprecedented mechanistic insights into RNA Pol II catalysis and reveal vital and evolutionarily conserved roles of water molecules in transcription.
リンク	Mol Cell / PubMed:42066756 / PubMed Central
手法	EM (単粒子)
解像度	1.96 - 3.12 Å
構造データ	53053 9qeb EMDB-53053, PDB-9qeb: CryoEM structure of transcribing RNA polymerase II elongation complex 手法: EM (単粒子) / 解像度: 2.26 Å EMDB-53056: CryoEM structure of transcribing RNA polymerase II elongation complex_Local density map of RPB4/7 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-53057: CryoEM structure of transcribing RNA polymerase II elongation complex_Composite map 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-53060: CryoEM structure of transcribing RNA polymerase II elongation complex_Local density map of RPB9 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-53062: CryoEM structure of transcribing RNA polymerase II elongation complex_Local density map of Jaw/RPB9 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-53063: CryoEM structure of transcribing RNA polymerase II elongation complex_Local density map of RPB12/Wall 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-53064: CryoEM structure of transcribing RNA polymerase II elongation complex_3D classification map containing the complete nucleic acid scaffold 手法: EM (単粒子) / 解像度: 3.12 Å 54374 9ryb EMDB-54374, PDB-9ryb: CryoEM structure of transcribing RNA polymerase II elongation complex in post-catalysis state 手法: EM (単粒子) / 解像度: 2.33 Å 55239 9sv6 EMDB-55239, PDB-9sv6: CryoEM structure of transcribing RNA polymerase II elongation complex with ATP and Elf1 手法: EM (単粒子) / 解像度: 1.96 Å EMDB-55240: CryoEM structure of transcribing RNA polymerase II elongation complex with ATP and Elf1_3D classification map containing the complete nucleic acid scaffold 手法: EM (単粒子) / 解像度: 2.38 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-K: Unknown entry ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-DPO*: DIPHOSPHATE
由来	saccharomyces cerevisiae (パン酵母)
キーワード	TRANSCRIPTION / CryoEM / RNA / Polymerase II / RNA Polymerase