EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Microtubules in Asgard archaea.
ジャーナル・号・ページ	Cell, Vol. 188, Issue 9, Page 2451-2464.e26, Year 2025
掲載日	2025年5月1日
著者	Florian Wollweber / Jingwei Xu / Rafael I Ponce-Toledo / Florina Marxer / Thiago Rodrigues-Oliveira / Anja Pössnecker / Zhen-Hao Luo / Jessie James Limlingan Malit / Anastasiia Kokhanovska / Michal Wieczorek / Christa Schleper / Martin Pilhofer /
PubMed 要旨	Microtubules are a hallmark of eukaryotes. Archaeal and bacterial homologs of tubulins typically form homopolymers and non-tubular superstructures. The origin of heterodimeric tubulins assembling ...Microtubules are a hallmark of eukaryotes. Archaeal and bacterial homologs of tubulins typically form homopolymers and non-tubular superstructures. The origin of heterodimeric tubulins assembling into microtubules remains unclear. Here, we report the discovery of microtubule-forming tubulins in Asgard archaea, the closest known relatives of eukaryotes. These Asgard tubulins (AtubA/B) are closely related to eukaryotic α/β-tubulins and the enigmatic bacterial tubulins BtubA/B. Proteomics of Candidatus Lokiarchaeum ossiferum showed that AtubA/B were highly expressed. Cryoelectron microscopy structures demonstrate that AtubA/B form eukaryote-like heterodimers, which assembled into 5-protofilament bona fide microtubules in vitro. The additional paralog AtubB2 lacks a nucleotide-binding site and competitively displaced AtubB. These AtubA/B2 heterodimers polymerized into 7-protofilament non-canonical microtubules. In a sub-population of Ca. Lokiarchaeum ossiferum cells, cryo-tomography revealed tubular structures, while expansion microscopy identified AtubA/B cytoskeletal assemblies. Our findings suggest a pre-eukaryotic origin of microtubules and provide a framework for understanding the fundamental principles of microtubule assembly.
リンク	Cell / PubMed:40120574
手法	EM (単粒子) / EM (らせん対称) / EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	3.3 - 23.0 Å
構造データ	50239 9f6t EMDB-50239, PDB-9f6t: cryoEM structure of Asgard tubulin heterodimer AtubA/B with GTP 手法: EM (単粒子) / 解像度: 3.5 Å 50240 9f6u EMDB-50240, PDB-9f6u: cryoEM structure of Asgard tubulin heterodimer AtubA/B with GDP 手法: EM (単粒子) / 解像度: 3.6 Å 50241 9f6v EMDB-50241, PDB-9f6v: cryoEM structure of Asgard tubulin heterodimer AtubA/B2 with GTP 手法: EM (単粒子) / 解像度: 3.5 Å 52463 9hxc EMDB-52463, PDB-9hxc: CryoEM structure of Asgard AtubA/B2 microtubule 手法: EM (らせん対称) / 解像度: 3.3 Å EMDB-52465: CryoEM structure of Asgard AtubA/B microtubule 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-52466: Cryo-tomogram of Asgard AtubA/B microtubule 手法: EM (トモグラフィー) EMDB-52467: Cryo-tomogram of Asgard AtubA/B2 microtubule 手法: EM (トモグラフィー) EMDB-52469: Sub-tomogram average of Asgard AtubA/B microtubule 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å EMDB-52470: Sub-tomogram average of Asgard AtubA/B2 microtubule 手法: EM (サブトモグラム平均) / 解像度: 23.0 Å EMDB-52471: Cryo-tomogram of Ca. L. ossiferum 手法: EM (トモグラフィー) EMDB-52472: Cryo-tomogram of Ca. L. ossiferum 手法: EM (トモグラフィー) EMDB-52473: Cryo-tomogram of Ca. L. ossiferum 手法: EM (トモグラフィー)
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM
由来	candidatus lokiarchaeum ossiferum (古細菌)
キーワード	CYTOSOLIC PROTEIN / cryoEM / tubulin / Asgard archaea / microtubule / STRUCTURAL PROTEIN / cytomotive filaments / cytoskeleton