EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Capsid restructuring activates semi-conservative dsRNA transcription in cystovirus ɸ6.
ジャーナル・号・ページ	Mol Cell, Vol. 86, Issue 2, Page 289-303.e5, Year 2026
掲載日	2026年1月8日
著者	Serban L Ilca / Xiaoyu Sun / Esa-Pekka Kumpula / Katri Eskelin / David I Stuart / Minna M Poranen / Juha T Huiskonen /
PubMed 要旨	Double-stranded (ds)RNA viruses replicate and transcribe their genome within a proteinaceous viral capsid to evade host cell defenses. While Reovirales members use conservative transcription, most ...Double-stranded (ds)RNA viruses replicate and transcribe their genome within a proteinaceous viral capsid to evade host cell defenses. While Reovirales members use conservative transcription, most dsRNA viruses, including cystoviruses, utilize semi-conservative transcription, in which a newly synthesized positive strand replaces the parental positive strand, which is released as mRNA. Here, we visualize semi-conservative transcription activation in cystovirus ɸ6 double-layered particles using cryogenic electron microscopy. We observe nucleotide-triggered disassembly of the domain-swapped outer capsid layer, subsequent expansion of the inner capsid layer, and stepwise assembly of transcription complexes at the opposing poles of the spooled dsRNA genome. These complexes consist of the viral polymerases embedded into a triskelion formed by the minor protein P7, which we show as essential for continuous transcription. The packaging hexamers proximal to the transcription sites channel the viral mRNA exit. Our results define the complex molecular pathway from the quiescent state to activated semi-conservative transcription.
リンク	Mol Cell / PubMed:41512857
手法	EM (単粒子)
解像度	3.5 - 8.0 Å
構造データ	EMDB-52379: Transcribing double-layered particle of bacteriophage phi6 reconstructed with icosahedral symmetry 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-52380: Transcribing single-layered particle of bacteriophage phi6 reconstructed with icosahedral symmetry 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-52381: Localized reconstruction of packaging hexamer P4 from polar region of transcribing single-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-52382: Localized reconstruction of packaging hexamer P4 from equatorial region of transcribing single-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-52383: Localized reconstruction of packaging hexamer P4 from polar region of transcribing double-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-52384: Localized reconstruction of packaging hexamer P4 from equatorial region of transcribing double-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 4.9 Å 52385 9htw EMDB-52385: Localized reconstruction consensus map of minor protein P7 from transcribing particles of bacteriophage phi6 PDB-9htw: Minor protein P7 dimer from transcribing particles of bacteriophage phi6 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-52386: Localized reconstruction of polymerase P2 from transcribing double-layered particle of bacteriophage phi6 in stage A1 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-52387: Localized reconstruction of polymerase P2 from transcribing double-layered particle of bacteriophage phi6 in stage A2 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-52388: Localized reconstruction of polymerase P2 from transcribing double-layered particle of bacteriophage phi6 in stage A3 手法: EM (単粒子) / 解像度: 4.7 Å 52389 9i5c 9i7w EMDB-52389: Localized reconstruction of polymerase P2 from transcribing single-layered particle of bacteriophage phi6 in stage B PDB-9i5c: Inner layer protein P1 chains in transcribing particles of bacteriophage phi6 PDB-9i7w: Extended and wrapped protein P7 dimers of dimers, the P1 layer and the RNA-dependent RNA polymerase P2 in transcribing particles of bacteriophage phi6 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-52390: Localized reconstruction of polymerase P2 from transcribing single-layered particle of bacteriophage phi6 in stage C 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-52391: Asymmetric reconstruction of transcribing double-layered particle of bacteriophage phi6, disassembly intermediate 1 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-52392: Asymmetric reconstruction of transcribing double-layered particle of bacteriophage phi6, disassembly intermediate 2 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-52393: Asymmetric reconstruction of transcribing double-layered particle of bacteriophage phi6, disassembly intermediate 3 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-52394: Asymmetric reconstruction of transcribing double-layered particle of bacteriophage phi6, disassembly intermediate 4 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-52395: Transcription-arrested double-layered particle of bacteriophage phi6 reconstructed with icosahedral symmetry 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-52396: Transcription-arrested single-layered particle of bacteriophage phi6 reconstructed with icosahedral symmetry 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-52397: Transcription-arrested double-layered particle of bacteriophage phi6 reconstructed with D3 symmetry 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-52398: Transcription-arrested single-layered particle of bacteriophage phi6 reconstructed with D3 symmetry 手法: EM (単粒子) / 解像度: 4.0 Å 52399 9hu4 EMDB-52399: Localized reconstruction of asymmetric unit from transcribing double-layered particle of bacteriophage phi6 PDB-9hu4: Asymmetric unit from transcribing double-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 3.5 Å 52400 9hu5 EMDB-52400: Localized reconstruction of asymmetric unit from transcribing single-layered particle of bacteriophage phi6 PDB-9hu5: Asymmetric unit from transcribing single-layered particle of bacteriophage phi6 手法: EM (単粒子) / 解像度: 3.7 Å 52401 9hu6 EMDB-52401: Localized reconstruction of asymmetric unit from transcribing single-layered particle of bacteriophage phi6 in an over-expanded state PDB-9hu6: Asymmetric unit from transcribing single-layered particle of bacteriophage phi6 in an over-expanded state 手法: EM (単粒子) / 解像度: 3.5 Å
由来	cystovirus phi6 (ウイルス) synthetic construct (人工物)
キーワード	VIRUS / cystovirus / phi6 / semi-conservative transcription / cryo-EM / cryogenic electron microscopy / localized reconstruction / symmetry relaxation / dsRNA virus / bacteriophage / VIRAL PROTEIN / structural protein / inner protein layer / transcribing particle / inner protein capsid / transcribing phi6 particle