EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dipeptidase 1 is a functional receptor for a porcine coronavirus.
ジャーナル・号・ページ	Nat Microbiol, Vol. 10, Issue 11, Page 2981-2996, Year 2025
掲載日	2025年10月10日
著者	Jérémy Dufloo / Ignacio Fernández / Atousa Arbabian / Ahmed Haouz / Nigel Temperton / Luis G Gimenez-Lirola / Félix A Rey / Rafael Sanjuán /
PubMed 要旨	Coronaviruses of the subgenus Embecovirus include several important pathogens, such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus and porcine haemagglutinating ...Coronaviruses of the subgenus Embecovirus include several important pathogens, such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus and porcine haemagglutinating encephalomyelitis virus (PHEV). While sialic acid is thought to be required for embecovirus entry, protein receptors remain unknown for most of these viruses. Here we show that PHEV does not require sialic acid for entry and instead uses dipeptidase 1 (DPEP1) as a receptor. Cryo-electron microscopy at 3.4-4.4 Å resolution revealed that, unlike other embecoviruses, PHEV displays both open and closed conformations of its spike trimer at steady state. The spike receptor-binding domain (RBD) exhibits extremely high sequence variability across embecoviruses, and we found that DPEP1 usage is specific to PHEV. In contrast, the X-ray structure of the RBD-DPEP1 complex at 2.25 Å showed that the structural elements involved in receptor binding are conserved, highlighting the remarkable versatility of this structural organization in adopting novel receptor specificities.
リンク	Nat Microbiol / PubMed:41073662 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.25 - 4.1 Å
構造データ	51827 9h3j EMDB-51827, PDB-9h3j: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike in the closed conformation, apo state 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-51844: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-51845: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the 2-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-51846: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the open conformation 手法: EM (単粒子) / 解像度: 3.9 Å 53679 9r6o EMDB-53679, PDB-9r6o: Local refinement of the N-terminal domain (NTD) and receptor binding domain (RBD) from the Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike in the closed conformation 手法: EM (単粒子) / 解像度: 4.1 Å 53680 9r6p EMDB-53680, PDB-9r6p: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the closed conformation bound to 9-O-Ac-Sia. 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53681: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the 1-RBD-up conformation, bound to 9-O-Ac-Sia 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-53682: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the 2-RBD-up conformation, bound to 9-O-Ac-Sia 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53683: Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike protein in the open conformation, bound to 9-O-Ac-Sia 手法: EM (単粒子) / 解像度: 3.0 Å 53684 9r6q EMDB-53684, PDB-9r6q: Local refinement of the N-terminal domain (NTD) and receptor binding domain (RBD) from the Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike in the closed conformation bound to 9-O-Ac-Sia 手法: EM (単粒子) / 解像度: 3.5 Å 53685 9r6r EMDB-53685, PDB-9r6r: Local refinement of the N-terminal domain (NTD) from the Porcine hemagglutinating encephalomyelitis virus (PHEV) Spike in the open conformation bound to 9-O-Ac-Sia 手法: EM (単粒子) / 解像度: 3.3 Å PDB-9h0b: Crystal structure of the Porcine Hemagglutinating Encephalomyelitis Virus (PHEV) receptor binding domain in complex with porcine DPEP1. 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER ChemComp-5N6: 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid / Neu5,9Ac2
由来	porcine hemagglutinating encephalomyelitis virus (ブタ赤血球凝集性脳脊髄炎ウイルス) Porcine hemagglutinating encephalomyelitis virus sus scrofa (ブタ)
キーワード	VIRAL PROTEIN / RBD Receptor Spike Entry / Coronavirus / Spike / Entry / glycoprotein