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- PDB-9h0b: Crystal structure of the Porcine Hemagglutinating Encephalomyelit... -

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Basic information

Entry
Database: PDB / ID: 9h0b
TitleCrystal structure of the Porcine Hemagglutinating Encephalomyelitis Virus (PHEV) receptor binding domain in complex with porcine DPEP1.
Components
  • Dipeptidase 1
  • Spike glycoprotein
KeywordsVIRAL PROTEIN / RBD Receptor Spike Entry
Function / homology
Function and homology information


Aflatoxin activation and detoxification / Synthesis of Leukotrienes (LT) and Eoxins (EX) / leukotriene D4 catabolic process / membrane dipeptidase / antibiotic metabolic process / GPI anchor binding / glutathione catabolic process / modified amino acid binding / homocysteine metabolic process / leukotriene metabolic process ...Aflatoxin activation and detoxification / Synthesis of Leukotrienes (LT) and Eoxins (EX) / leukotriene D4 catabolic process / membrane dipeptidase / antibiotic metabolic process / GPI anchor binding / glutathione catabolic process / modified amino acid binding / homocysteine metabolic process / leukotriene metabolic process / dipeptidase activity / metallodipeptidase activity / microvillus membrane / side of membrane / cellular response to nitric oxide / cellular response to calcium ion / negative regulation of cell migration / beta-lactamase activity / beta-lactamase / cellular response to insulin stimulus / apical part of cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated virion attachment to host cell / apical plasma membrane / endocytosis involved in viral entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / endoplasmic reticulum membrane / host cell plasma membrane / virion membrane / proteolysis / extracellular space / zinc ion binding / identical protein binding / membrane / plasma membrane
Similarity search - Function
Membrane dipeptidase, active site / Renal dipeptidase active site. / Spike (S) protein S1 subunit, receptor-binding domain, HEV / Peptidase M19 / Membrane dipeptidase (Peptidase family M19) / Renal dipeptidase family profile. / Spike (S) protein S1 subunit, N-terminal domain, murine hepatitis virus-like / Spike glycoprotein S2, coronavirus, C-terminal / Coronavirus spike glycoprotein S2, intravirion / Metal-dependent hydrolase ...Membrane dipeptidase, active site / Renal dipeptidase active site. / Spike (S) protein S1 subunit, receptor-binding domain, HEV / Peptidase M19 / Membrane dipeptidase (Peptidase family M19) / Renal dipeptidase family profile. / Spike (S) protein S1 subunit, N-terminal domain, murine hepatitis virus-like / Spike glycoprotein S2, coronavirus, C-terminal / Coronavirus spike glycoprotein S2, intravirion / Metal-dependent hydrolase / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, N-terminal domain superfamily / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Spike glycoprotein, betacoronavirus / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2
Similarity search - Domain/homology
Dipeptidase 1 / Spike glycoprotein
Similarity search - Component
Biological speciesSus scrofa (pig)
Porcine hemagglutinating encephalomyelitis virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.25 Å
AuthorsFernandez, I. / Rey, F.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Microbiol / Year: 2025
Title: Dipeptidase 1 is a functional receptor for a porcine coronavirus.
Authors: Jérémy Dufloo / Ignacio Fernández / Atousa Arbabian / Ahmed Haouz / Nigel Temperton / Luis G Gimenez-Lirola / Félix A Rey / Rafael Sanjuán /
Abstract: Coronaviruses of the subgenus Embecovirus include several important pathogens, such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus and porcine haemagglutinating ...Coronaviruses of the subgenus Embecovirus include several important pathogens, such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus and porcine haemagglutinating encephalomyelitis virus (PHEV). While sialic acid is thought to be required for embecovirus entry, protein receptors remain unknown for most of these viruses. Here we show that PHEV does not require sialic acid for entry and instead uses dipeptidase 1 (DPEP1) as a receptor. Cryo-electron microscopy at 3.4-4.4 Å resolution revealed that, unlike other embecoviruses, PHEV displays both open and closed conformations of its spike trimer at steady state. The spike receptor-binding domain (RBD) exhibits extremely high sequence variability across embecoviruses, and we found that DPEP1 usage is specific to PHEV. In contrast, the X-ray structure of the RBD-DPEP1 complex at 2.25 Å showed that the structural elements involved in receptor binding are conserved, highlighting the remarkable versatility of this structural organization in adopting novel receptor specificities.
History
DepositionOct 8, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 6, 2025Provider: repository / Type: Initial release
Revision 1.1Oct 22, 2025Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Nov 12, 2025Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: Dipeptidase 1
A: Spike glycoprotein
C: Dipeptidase 1
D: Spike glycoprotein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,53128
Polymers144,3174
Non-polymers4,21424
Water9,116506
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)96.720, 73.010, 136.620
Angle α, β, γ (deg.)90.000, 91.940, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1chain "D"
d_1ens_2chain "B"
d_2ens_2chain "C"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1ASPASPTHRTHRAB327 - 6031 - 277
d_12ens_1NAGNAGNAGNAGAR701
d_13ens_1NAGNAGNAGNAGAS702
d_21ens_1ASPASPTHRTHRDD327 - 6031 - 277
d_22ens_1NAGNAGNAGNAGDY701
d_23ens_1NAGNAGNAGNAGDZ702
d_11ens_2ASPASPSERSERBA17 - 3841 - 368
d_12ens_2NAGNAGNAGNAGEE1
d_13ens_2NAGNAGNAGNAGEE2
d_14ens_2NAGNAGNAGNAGFF1
d_15ens_2NAGNAGNAGNAGFF2
d_16ens_2FUCFUCFUCFUCFF3
d_17ens_2FUCFUCFUCFUCEE3
d_21ens_2ASPASPSERSERCC17 - 3841 - 368
d_22ens_2NAGNAGNAGNAGGG1
d_23ens_2NAGNAGNAGNAGGG2
d_24ens_2NAGNAGNAGNAGHH1
d_25ens_2NAGNAGNAGNAGHH2
d_26ens_2FUCFUCFUCFUCHH3
d_27ens_2FUCFUCFUCFUCGG3

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(0.998946639943, -0.0357651485265, -0.0287482990415), (-0.035650348554, -0.999354210245, 0.00449612197657), (-0.0288905381548, -0.00346649906011, -0.999576570448)2.29541059993, -12.3766298978, 70.6491974077
2given(0.996014019931, -0.0533196874241, 0.0715058251797), (-0.0534228931332, -0.998571867134, -0.000469741871219), (0.0714287518504, -0.00335217856749, -0.997440071537)-2.7790889908, -11.8297968602, 68.5052033787

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Components

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Protein , 2 types, 4 molecules BCAD

#1: Protein Dipeptidase 1 / Beta-lactamase / Microsomal dipeptidase / Renal dipeptidase


Mass: 41029.996 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sus scrofa (pig) / Gene: DPEP1, RDP / Cell line (production host): S2 / Production host: Drosophila melanogaster (fruit fly)
References: UniProt: P22412, membrane dipeptidase, beta-lactamase
#2: Protein Spike glycoprotein / S glycoprotein / E2 / Peplomer protein


Mass: 31128.750 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Porcine hemagglutinating encephalomyelitis virus
Cell line (production host): S2 / Production host: Drosophila melanogaster (fruit fly) / References: UniProt: Q2QKN3

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Sugars , 2 types, 8 molecules

#3: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE
#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 522 molecules

#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Zn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 506 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.34 Å3/Da / Density % sol: 63.18 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 0.1 M MES (pH 6.0), 0.2 M zinc acetate, 10% w/v PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.9801 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: May 31, 2024
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9801 Å / Relative weight: 1
ReflectionResolution: 2.25→48.33 Å / Num. obs: 90076 / % possible obs: 99.6 % / Redundancy: 6.9 % / CC1/2: 0.984 / Rmerge(I) obs: 0.238 / Net I/σ(I): 8.4
Reflection shellResolution: 2.25→2.28 Å / Redundancy: 6.7 % / Mean I/σ(I) obs: 1 / Num. unique obs: 6350 / CC1/2: 0.454 / % possible all: 95.7

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Processing

Software
NameVersionClassification
PHENIX1.21.2_5419refinement
XDSdata reduction
Aimlessdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.25→48.33 Å / SU ML: 0.3518 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.4621
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2348 4502 5 %
Rwork0.198 85564 -
obs0.1999 90066 99.62 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.82 Å2
Refinement stepCycle: LAST / Resolution: 2.25→48.33 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9896 0 224 506 10626
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.005110328
X-RAY DIFFRACTIONf_angle_d0.7514044
X-RAY DIFFRACTIONf_chiral_restr0.04831594
X-RAY DIFFRACTIONf_plane_restr0.00631828
X-RAY DIFFRACTIONf_dihedral_angle_d11.9543946
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2BAX-RAY DIFFRACTIONTorsion NCS0.407565767191
ens_2d_2ABX-RAY DIFFRACTIONTorsion NCS0.258582352429
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.25-2.280.47431350.39142580X-RAY DIFFRACTION90.2
2.28-2.30.39861490.35232827X-RAY DIFFRACTION99.93
2.3-2.330.34771480.33162819X-RAY DIFFRACTION99.93
2.33-2.360.32891510.31012866X-RAY DIFFRACTION99.9
2.36-2.390.32631490.29522814X-RAY DIFFRACTION99.93
2.39-2.430.28811490.26932862X-RAY DIFFRACTION99.83
2.43-2.460.30271500.26052850X-RAY DIFFRACTION99.97
2.46-2.50.31151510.26092853X-RAY DIFFRACTION99.93
2.5-2.540.28631510.23982874X-RAY DIFFRACTION99.97
2.54-2.580.2871490.23662836X-RAY DIFFRACTION100
2.58-2.620.25121490.22822818X-RAY DIFFRACTION99.97
2.62-2.670.28261480.2272821X-RAY DIFFRACTION99.93
2.67-2.720.26811510.232858X-RAY DIFFRACTION99.97
2.72-2.780.27031500.23352863X-RAY DIFFRACTION100
2.78-2.840.27621490.23662825X-RAY DIFFRACTION99.93
2.84-2.90.28191520.24822877X-RAY DIFFRACTION99.97
2.9-2.980.27141490.21492841X-RAY DIFFRACTION100
2.98-3.060.28371500.2142852X-RAY DIFFRACTION100
3.06-3.150.25131520.2042876X-RAY DIFFRACTION100
3.15-3.250.23921500.20122847X-RAY DIFFRACTION100
3.25-3.360.22041500.19412868X-RAY DIFFRACTION99.97
3.36-3.50.25981520.19342876X-RAY DIFFRACTION100
3.5-3.660.22021510.18582864X-RAY DIFFRACTION99.97
3.66-3.850.21571510.1682870X-RAY DIFFRACTION99.97
3.85-4.090.18891510.15372871X-RAY DIFFRACTION100
4.09-4.410.15921510.14182880X-RAY DIFFRACTION99.93
4.41-4.850.17221530.14162903X-RAY DIFFRACTION99.93
4.85-5.550.18841510.15982872X-RAY DIFFRACTION99.97
5.55-6.990.19881540.17682914X-RAY DIFFRACTION100
6.99-48.330.19911560.18052987X-RAY DIFFRACTION99.53
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.8957043442.22217369505-3.266204439183.32104392089-2.183590009984.40812627405-0.04854157874480.480642908499-0.147810159947-0.3455256615170.00443787594371-0.1412843304690.410267247248-0.2107030504580.1167090179580.4191625663770.0429583239531-0.06195221992790.264561721201-0.03347592684830.2458682366557.17593945766-15.461660447711.0022556066
20.75665249108-0.573836493321-0.8066309232041.587693205920.2716860555482.289083502860.08344860747220.1313167750560.0791583949742-0.161546175984-0.0122282488008-0.08379610578310.165505171442-0.0747440515895-0.0666450317360.3354046306650.0102017700406-0.04190925331310.2892101298390.02413856969920.2812551595054.74388664556-9.7241624108221.5481295162
31.76536161251-0.1805834121281.271558012150.4067177740590.3381969003634.19921831210.1172542686920.07272373820350.133986736113-0.269171767590.0008697319842710.0856744198294-0.353521596762-0.250337426362-0.09673242019670.4451180500930.048260879238-0.01324836981580.237246643490.08625262846240.305515944011-4.146902018437.7877275347115.8147340513
41.453633893980.2162611688830.2497585264150.9941933245740.3842236247882.805042103750.1139707124150.2148635689610.117475663413-0.4423239848530.00536354329671-0.05353969015530.02426526990820.019753947614-0.1103083614250.5330540809730.04159750554470.01158274389850.2948787303540.05743615925230.2976930431316.6072973329-1.436978182363.10996252053
56.728860435763.33976607639-1.566362057914.0963529664-1.20489368621.90840018137-0.1730476393370.06390738007410.351346967421-0.1149981269330.2514709512730.521264642021-0.349143176735-0.269662404666-0.08594242370870.3157369020130.0719680117398-0.03833457790610.2822140827180.0002527781410440.250364942091-6.620961884977.920843783735.9408310913
63.815828831070.821712841563-1.348587560682.156690666591.353689379824.05298078330.0558135641742-0.0519480758751-0.18520344549-0.295422322435-0.00955366818575-0.5434056478440.5196899928780.621810226358-0.06216052892150.5120772957790.156431461678-0.01112847044250.5721718254470.05711752189060.42669136828167.7173141659-37.035878369324.8369696247
74.43415676427-3.349668272191.802609067293.40105456573-1.854640699820.8957937893440.02833829308180.1436108360530.3254503474220.0353123448109-0.0871407841702-0.242109619741-0.0980617943803-0.01464546001180.06758052351860.3678207690960.0410122616850.0180673007970.4478838652950.02973894492780.3742544496747.7895411693-18.598074220922.7890126288
81.99998613987-5.090896988547.668581951351.999996206781.873208186572.000012315891.59674364175-7.089658597343.60811608574-3.066580851721.972087054934.32181696404-1.22082090083.7025599926-3.59623852381.00372865940.32915159485-0.1396111155621.18641793801-0.3509499731810.80473956266476.6585812078-46.281038902123.0878713341
92.61044742702-1.505374427162.520590466393.76281286605-2.048552002525.034085062950.060700824792-0.1140368940260.1319169570320.126587493825-0.0283127916236-0.0754983054389-0.249615801469-0.100836763714-0.0166623333990.190399959432-0.02229167827840.03243704255370.225186402721-0.02926101569930.2941555643416.031415331263.2524806838858.0783769533
10-0.03894520350740.1220515646910.2018540586481.58082285940.1473128103181.833152688920.055607069190.006485224034420.041468100364-0.0385986144935-0.0152079798032-0.0316663992303-0.0199800555515-0.0042577504503-0.03975000423240.248091153421-0.00212442617036-0.01545321409140.2645880784470.01636610749030.3102501343684.04703739022-2.3356254724647.3688547967
111.57500096626-0.253600635638-1.582845595010.9320828763450.3718649501894.645339705710.06104256216680.112813861021-0.1127042692770.0204389616599-0.0169955833530.1393075539080.296762798124-0.311236720103-0.03677011951630.305557637646-0.0442323240811-0.07509023273790.2294156724480.03816564290640.321452622902-6.18655748897-19.345257471952.4533235875
120.769218044034-0.518368713012-0.05455957193432.086931609580.3305641628851.572633329730.0165134764544-0.096550927668-0.0345638789330.3089974009190.0423188860961-0.09733768541650.03688723767270.0236992654298-0.06286264253390.283294618167-0.0217540103781-0.06288654829650.2388492486210.02092983961770.2900890449014.11545010142-10.660424221865.8981676144
136.33023092092-1.401848489371.765935865772.29808623445-0.8483128874972.59959413870.03883217846630.213193403494-0.382136649375-0.2178691327490.142107418970.3105271229650.287234808985-0.277277695713-0.2036576362880.346147991198-0.0393939585692-0.06360683442150.330364348628-0.02465277182890.303301568392-7.25078955531-19.387526203832.176270293
143.42907382599-0.3035530472541.74837254252.79283433755-0.1934935661654.10169391660.1118712071220.3255133605220.1049148712870.125989537236-0.121476088759-0.312006364951-0.286880528950.685479497331-0.01196818560680.298152017638-0.0557270748102-0.03501704368320.510975805583-0.0180225636990.41663030926565.015723420721.315173854742.888744267
153.34294881732.65600611975-0.7668178172921.77221027608-0.8795332937170.5321549497970.00588701594649-0.0574150900301-0.321176557798-0.0195552577201-0.0954642858908-0.2415668579470.02559556221240.06350825178770.09711586221170.3150134566350.0281558613623-0.1013170456490.349927483465-0.04528369584790.53838709818246.30308579753.6735882671747.1263816002
161.999990921871.999970499996.128549663981.999982494172.000012933472.00000629438-5.43455687979-1.364615904916.348436843085.299748491414.48863101655-7.34947009298-0.0304048145198-1.938693895110.9859935888560.851603053128-0.0734582513864-0.1972942473561.53745530108-0.2346809533251.0072215821675.96724187331.835874537844.8758532602
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'C' and (resid 17 through 57 )CC17 - 571 - 41
22chain 'C' and (resid 58 through 165 )CC58 - 16542 - 149
33chain 'C' and (resid 166 through 249 )CC166 - 249150 - 233
44chain 'C' and (resid 250 through 356 )CC250 - 356234 - 340
55chain 'C' and (resid 357 through 384 )CC357 - 384341 - 368
66chain 'D' and (resid 327 through 388 )DD327 - 3881 - 62
77chain 'D' and (resid 389 through 602 )DD389 - 60263 - 276
88chain 'D' and (resid 603 through 603 )DD603277
99chain 'B' and (resid 17 through 57 )BA17 - 571 - 41
1010chain 'B' and (resid 58 through 165 )BA58 - 16542 - 149
1111chain 'B' and (resid 166 through 249 )BA166 - 249150 - 233
1212chain 'B' and (resid 250 through 356 )BA250 - 356234 - 340
1313chain 'B' and (resid 357 through 384 )BA357 - 384341 - 368
1414chain 'A' and (resid 327 through 405 )AH327 - 4051 - 79
1515chain 'A' and (resid 406 through 602 )AH406 - 60280 - 276
1616chain 'A' and (resid 603 through 603 )AH603277

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