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TitleNon-nucleosomal (CENP-A/H4) - DNA complexes as a possible platform for centromere organization.
Journal, issue, pagesbioRxiv, Year 2025
Publish dateJan 1, 2025
AuthorsAhmad Ali-Ahmad / Mira Mors / Manuel Carrer / Xinmeng Li / Silvija Bilokapić / Mario Halić / Michele Cascella / Nikolina Sekulić /
PubMed AbstractThe centromere is a part of the chromosome that is essential for the even segregation of duplicated chromosomes during cell division. It is epigenetically defined by the presence of the histone H3 ...The centromere is a part of the chromosome that is essential for the even segregation of duplicated chromosomes during cell division. It is epigenetically defined by the presence of the histone H3 variant CENP-A. CENP-A associates specifically with a group of 16 proteins that form the centromere-associated network of proteins (CCAN). In mitosis, the kinetochore forms on the CCAN to connect the duplicated chromosomes to the microtubules protruding from the cell poles. Previous studies have shown that CENP-A replaces H3 in nucleosomes, and recently the structures of CENP-A-containing nucleosomes in complex with CCANs have been revealed, but they show only a limited interaction between CCANs and CENP-A. Here, we report the cryoEM structure of 2x(CENP-A/H4)-di-tetramers assembled on DNA in the absence of H2A/H2B histone dimer and speculate how (CENP-A/H4)-tetramers and -di-tetramers might serve as a platform for CCAN organization.
External linksbioRxiv / PubMed:39803555 / PubMed Central
MethodsEM (single particle)
Resolution3.75 - 7.01 Å
Structure data

51645

9gxa

EMDB-51645, PDB-9gxa:
CENP-A/H4 di-tetrasome assembled on alpha-satellite DNA.
Method: EM (single particle) / Resolution: 4.01 Å

EMDB-51646: H3/H4 di-tetrasome assembled on alpha-satellite DNA (closed conformation).
Method: EM (single particle) / Resolution: 3.82 Å

EMDB-51647: H3/H4 di-tetrasome assembled on alpha-satellite DNA (open conformation).
Method: EM (single particle) / Resolution: 3.75 Å

EMDB-51656: CENP-A/H4 tetra-tetrasome assembled on 342 bp alpha-satellite DNA.
Method: EM (single particle) / Resolution: 7.01 Å

Source
  • homo sapiens (human)
KeywordsDNA BINDING PROTEIN / CENP-A/H4 / di-tetrasome / nucleosome / centromere / CENP-A / Human / Cell division.

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