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-Structure paper
タイトル | Non-nucleosomal (CENP-A/H4) - DNA complexes as a possible platform for centromere organization. |
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ジャーナル・号・ページ | bioRxiv, Year 2025 |
掲載日 | 2025年1月1日 |
![]() | Ahmad Ali-Ahmad / Mira Mors / Manuel Carrer / Xinmeng Li / Silvija Bilokapić / Mario Halić / Michele Cascella / Nikolina Sekulić / ![]() ![]() |
PubMed 要旨 | The centromere is a part of the chromosome that is essential for the even segregation of duplicated chromosomes during cell division. It is epigenetically defined by the presence of the histone H3 ...The centromere is a part of the chromosome that is essential for the even segregation of duplicated chromosomes during cell division. It is epigenetically defined by the presence of the histone H3 variant CENP-A. CENP-A associates specifically with a group of 16 proteins that form the centromere-associated network of proteins (CCAN). In mitosis, the kinetochore forms on the CCAN to connect the duplicated chromosomes to the microtubules protruding from the cell poles. Previous studies have shown that CENP-A replaces H3 in nucleosomes, and recently the structures of CENP-A-containing nucleosomes in complex with CCANs have been revealed, but they show only a limited interaction between CCANs and CENP-A. Here, we report the cryoEM structure of 2x(CENP-A/H4)-di-tetramers assembled on DNA in the absence of H2A/H2B histone dimer and speculate how (CENP-A/H4)-tetramers and -di-tetramers might serve as a platform for CCAN organization. |
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手法 | EM (単粒子) |
解像度 | 3.75 - 7.01 Å |
構造データ | EMDB-51645, PDB-9gxa: ![]() EMDB-51646: H3/H4 di-tetrasome assembled on alpha-satellite DNA (closed conformation). ![]() EMDB-51647: H3/H4 di-tetrasome assembled on alpha-satellite DNA (open conformation). ![]() EMDB-51656: CENP-A/H4 tetra-tetrasome assembled on 342 bp alpha-satellite DNA. |
由来 |
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![]() | DNA BINDING PROTEIN / CENP-A/H4 / di-tetrasome / nucleosome / centromere / CENP-A / Human / Cell division. |