EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Influenza a virus antiparallel helical nucleocapsid-like pseudo-atomic structure.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 53, Issue 3, Year 2025
掲載日	2025年1月24日
著者	Florian Chenavier / Eleftherios Zarkadas / Lily-Lorette Freslon / Alice J Stelfox / Guy Schoehn / Rob W H Ruigrok / Allison Ballandras-Colas / Thibaut Crépin /
PubMed 要旨	Influenza A viruses are responsible for human seasonal epidemics and severe animal pandemics with a risk of zoonotic transmission to humans. The viral segmented RNA genome is encapsidated by ...Influenza A viruses are responsible for human seasonal epidemics and severe animal pandemics with a risk of zoonotic transmission to humans. The viral segmented RNA genome is encapsidated by nucleoproteins (NP) and attached to the heterotrimeric polymerase, forming the viral ribonucleoproteins (vRNPs). Flexible helical vRNPs are central for viral transcription and replication. In this study, we present an advanced biological tool, the antiparallel helical RNP-like complex, assembled from recombinant N-terminally truncated NP and short synthetic RNA. The 3.0 Å cryo-electron microscopy structure details for the first time the whole RNA pathway across NP as well as NP-NP interactions that drive the antiparallel helical assembly accommodating major and minor grooves. Our findings show that the surface of the protein can harbour several conformations of the RNA, confirming that the number of nucleobases that binds to NP is not fixed, but ranges probably between 20 and 24. Taking all together, our data provide details to further understand the genome encapsidation and explain the inherent flexibility of influenza A virus vRNPs.
リンク	Nucleic Acids Res / PubMed:39673795 / PubMed Central
手法	EM (らせん対称)
解像度	3.04 - 4.0 Å
構造データ	51184 9gan EMDB-51184, PDB-9gan: CryoEM structure of influenza A RNP-like particle single-stranded assembled with a 12-mer RNA. 手法: EM (らせん対称) / 解像度: 3.32 Å 51185 9gap EMDB-51185, PDB-9gap: CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 12-mer RNA. 手法: EM (らせん対称) / 解像度: 4.0 Å 51186 9gaq EMDB-51186, PDB-9gaq: CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 14-mer RNA. 手法: EM (らせん対称) / 解像度: 3.6 Å 51187 9gas EMDB-51187, PDB-9gas: Focused reconstruction on strand 2 of the influenza A RNP-like particle double-stranded assembled with a 18-mer RNA. 手法: EM (らせん対称) / 解像度: 3.08 Å 51188 9gat EMDB-51188, PDB-9gat: CryoEM structure of the antiparallel double-stranded influenza A RNP-like particle with a 18-mer RNA. 手法: EM (らせん対称) / 解像度: 3.2 Å 51189 9gav EMDB-51189, PDB-9gav: Focused reconstruction on strand 1 of the influenza A RNP-like particle double-stranded assembled with a 18-mer RNA. 手法: EM (らせん対称) / 解像度: 3.04 Å
化合物	PDB-1ijk: The von Willebrand Factor mutant (I546V) A1 domain-botrocetin Complex
由来	influenza a virus (A型インフルエンザウイルス) synthetic construct (人工物)
キーワード	VIRAL PROTEIN / Nucleoprotein / RNA / complex / RNP-like / influenza virus