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TitleOptimal functioning of the Lpt bridge depends on a ternary complex between the lipocalin YedD and the LptDE translocon.
Journal, issue, pagesCell Rep, Vol. 44, Issue 4, Page 115446, Year 2025
Publish dateMar 23, 2025
AuthorsAlexandra Gennaris / Van Son Nguyen / Laurie Thouvenel / Naemi Csoma / Didier Vertommen / Bogdan Iuliu Iorga / Han Remaut / Jean-François Collet /
PubMed AbstractThe outer membrane is an efficient permeability barrier that protects gram-negative bacteria against external assaults, including many antibiotics. The unique permeability features of the outer ...The outer membrane is an efficient permeability barrier that protects gram-negative bacteria against external assaults, including many antibiotics. The unique permeability features of the outer membrane are due to the presence of lipopolysaccharide (LPS) molecules in its outer leaflet. LPS transport relies on the essential lipopolysaccharide transport (Lpt) pathway, which forms a bridge from the inner to the outer membrane. The LptDE translocon inserts LPS into the outer leaflet. Here, we identify the lipocalin YedD as a component of the translocon. Cryoelectron microscopy of the YedD-LptDE complex reveals that YedD binds LptD at a critical interface between its β-barrel and periplasmic β-taco domain. The YedD-LptDE complex is functionally relevant: under conditions where the connectivity of the β-taco and Lpt bridge is compromised, the absence of YedD decreases cell viability and causes LPS accumulation in the inner membrane. Our findings establish YedD as an Lpt component required for optimal LPS transport.
External linksCell Rep / PubMed:40127101
MethodsEM (single particle)
Resolution3.57 Å
Structure data

50893

9fz5

EMDB-50893, PDB-9fz5:
Cryo-EM structure of LptDE-YedD complex from Escherichia Coli
Method: EM (single particle) / Resolution: 3.57 Å

Source
  • escherichia coli k-12 (bacteria)
KeywordsMEMBRANE PROTEIN / Outer membrane protein / lipopolysaccharide transport (Lpt) complex / Lipid binding complex / LptDE Translocon / Lipocalin YedD.

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