EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 348, Year 2025
掲載日	2025年1月2日
著者	Gabrielle Bourgeois / Pierre-Damien Coureux / Christine Lazennec-Schurdevin / Clément Madru / Thomas Gaillard / Magalie Duchateau / Julia Chamot-Rooke / Sophie Bourcier / Yves Mechulam / Emmanuelle Schmitt /
PubMed 要旨	The archaeal ribosome is of the eukaryotic type. TACK and Asgard superphyla, the closest relatives of eukaryotes, have ribosomes containing eukaryotic ribosomal proteins not found in other archaea, ...The archaeal ribosome is of the eukaryotic type. TACK and Asgard superphyla, the closest relatives of eukaryotes, have ribosomes containing eukaryotic ribosomal proteins not found in other archaea, eS25, eS26 and eS30. Here, we investigate the case of Saccharolobus solfataricus, a TACK crenarchaeon, using mainly leaderless mRNAs. We characterize the small ribosomal subunit of S. solfataricus bound to SD-leadered or leaderless mRNAs. Cryo-EM structures show eS25, eS26 and eS30 bound to the small subunit. We identify two ribosomal proteins, aS33 and aS34, and an additional domain of eS6. Leaderless mRNAs are bound to the small subunit with contribution of their 5'-triphosphate group. Archaeal eS26 binds to the mRNA exit channel wrapped around the 3' end of rRNA, as in eukaryotes. Its position is not compatible with an SD:antiSD duplex. Our results suggest a positive role of eS26 in leaderless mRNAs translation and possible evolutionary routes from archaeal to eukaryotic translation.
リンク	Nat Commun / PubMed:39753558 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.7 Å
構造データ	50445 9fhl EMDB-50445, PDB-9fhl: High-resolution cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit bound to mRNA and initiator tRNA 手法: EM (単粒子) / 解像度: 2.5 Å 50709 9fra EMDB-50709, PDB-9fra: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA 手法: EM (単粒子) / 解像度: 2.8 Å 50716 9frk EMDB-50716, PDB-9frk: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA 手法: EM (単粒子) / 解像度: 3.0 Å 50717 9frl EMDB-50717, PDB-9frl: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA with h44 in up position 手法: EM (単粒子) / 解像度: 2.97 Å 50724 9fs6 EMDB-50724, PDB-9fs6: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA with h44 in up position 手法: EM (単粒子) / 解像度: 2.9 Å 50725 9fs8 EMDB-50725: Cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit with helix 44 in position down bound to mRNA and initiator tRNA PDB-9fs8: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aEF1A-like mRNA 手法: EM (単粒子) / 解像度: 3.7 Å 50727 9fsf EMDB-50727, PDB-9fsf: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA with h44 in up position 手法: EM (単粒子) / 解像度: 2.8 Å 50854 9fy0 EMDB-50854, PDB-9fy0: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SPM: SPERMINE / スペルミン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	saccharolobus solfataricus p2 (古細菌) escherichia coli (大腸菌) pyrococcus abyssi (古細菌) Saccharolobus solfataricus (古細菌)
キーワード	TRANSLATION / 30S / ribosome / Saccharolobus solfataricus / initiation / leaderless mRNA / Initiation complex / tRNA / mRNA / leaderless