Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.
Journal, issue, pages	Nat Commun, Vol. 16, Issue 1, Page 348, Year 2025
Publish date	Jan 2, 2025
Authors	Gabrielle Bourgeois / Pierre-Damien Coureux / Christine Lazennec-Schurdevin / Clément Madru / Thomas Gaillard / Magalie Duchateau / Julia Chamot-Rooke / Sophie Bourcier / Yves Mechulam / Emmanuelle Schmitt /
PubMed Abstract	The archaeal ribosome is of the eukaryotic type. TACK and Asgard superphyla, the closest relatives of eukaryotes, have ribosomes containing eukaryotic ribosomal proteins not found in other archaea, ...The archaeal ribosome is of the eukaryotic type. TACK and Asgard superphyla, the closest relatives of eukaryotes, have ribosomes containing eukaryotic ribosomal proteins not found in other archaea, eS25, eS26 and eS30. Here, we investigate the case of Saccharolobus solfataricus, a TACK crenarchaeon, using mainly leaderless mRNAs. We characterize the small ribosomal subunit of S. solfataricus bound to SD-leadered or leaderless mRNAs. Cryo-EM structures show eS25, eS26 and eS30 bound to the small subunit. We identify two ribosomal proteins, aS33 and aS34, and an additional domain of eS6. Leaderless mRNAs are bound to the small subunit with contribution of their 5'-triphosphate group. Archaeal eS26 binds to the mRNA exit channel wrapped around the 3' end of rRNA, as in eukaryotes. Its position is not compatible with an SD:antiSD duplex. Our results suggest a positive role of eS26 in leaderless mRNAs translation and possible evolutionary routes from archaeal to eukaryotic translation.
External links	Nat Commun / PubMed:39753558 / PubMed Central
Methods	EM (single particle)
Resolution	2.5 - 3.7 Å
Structure data	50445 9fhl EMDB-50445, PDB-9fhl: High-resolution cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit bound to mRNA and initiator tRNA Method: EM (single particle) / Resolution: 2.5 Å 50709 9fra EMDB-50709, PDB-9fra: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA Method: EM (single particle) / Resolution: 2.8 Å 50716 9frk EMDB-50716, PDB-9frk: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA Method: EM (single particle) / Resolution: 3.0 Å 50717 9frl EMDB-50717, PDB-9frl: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA with h44 in up position Method: EM (single particle) / Resolution: 2.97 Å 50724 9fs6 EMDB-50724, PDB-9fs6: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA with h44 in up position Method: EM (single particle) / Resolution: 2.9 Å 50725 9fs8 EMDB-50725: Cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit with helix 44 in position down bound to mRNA and initiator tRNA PDB-9fs8: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aEF1A-like mRNA Method: EM (single particle) / Resolution: 3.7 Å 50727 9fsf EMDB-50727, PDB-9fsf: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA with h44 in up position Method: EM (single particle) / Resolution: 2.8 Å 50854 9fy0 EMDB-50854, PDB-9fy0: Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA Method: EM (single particle) / Resolution: 2.9 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-SPM: SPERMINE ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM
Source	saccharolobus solfataricus p2 (archaea) escherichia coli (E. coli) pyrococcus abyssi (archaea) Saccharolobus solfataricus (archaea)
Keywords	TRANSLATION / 30S / ribosome / Saccharolobus solfataricus / initiation / leaderless mRNA / Initiation complex / tRNA / mRNA / leaderless