Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The structure of human sweetness.
Journal, issue, pages	Cell, Year 2025
Publish date	May 2, 2025
Authors	Zhang Juen / Zhengyuan Lu / Ruihuan Yu / Andrew N Chang / Brian Wang / Anthony W P Fitzpatrick / Charles S Zuker /
PubMed Abstract	In humans, the detection and ultimately the perception of sweetness begin in the oral cavity, where taste receptor cells (TRCs) dedicated to sweet-sensing interact with sugars, artificial sweeteners, ...In humans, the detection and ultimately the perception of sweetness begin in the oral cavity, where taste receptor cells (TRCs) dedicated to sweet-sensing interact with sugars, artificial sweeteners, and other sweet-tasting chemicals. Human sweet TRCs express on their cell surface a sweet receptor that initiates the cascade of signaling events responsible for our strong attraction to sweet stimuli. Here, we describe the cryo-electron microscopy (cryo-EM) structure of the human sweet receptor bound to two of the most widely used artificial sweeteners-sucralose and aspartame. Our results reveal the structural basis for sweet detection, provide insights into how a single receptor mediates all our responses to such a wide range of sweet-tasting compounds, and open up unique possibilities for designing a generation of taste modulators informed by the structure of the human receptor.
External links	Cell / PubMed:40339580
Methods	EM (single particle)
Resolution	2.8 - 4.3 Å
Structure data	EMDB-49605: Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the Cysteine-Rich domains (CR) from the combined datasets Method: EM (single particle) / Resolution: 3.3 Å EMDB-49606: Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the combined datasets Method: EM (single particle) / Resolution: 3.5 Å EMDB-49607: Consensus map of the human sweet taste receptor composed of TAS1R2 and TAS1R3 GPCRs from the combined datasets Method: EM (single particle) / Resolution: 3.7 Å 49608 9nor EMDB-49608, PDB-9nor: Human sweet taste receptor composed of TAS1R2 and TAS1R3 GPCRs from the combined datasets Method: EM (single particle) / Resolution: 3.4 Å EMDB-49609: Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the cysteine-rich domains (CR) from the PEG400 dataset Method: EM (single particle) / Resolution: 3.3 Å EMDB-49610: Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the PEG400 dataset Method: EM (single particle) / Resolution: 3.8 Å EMDB-49611: Consensus map of the human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset Method: EM (single particle) / Resolution: 3.7 Å 49612 9nos EMDB-49612, PDB-9nos: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset Method: EM (single particle) / Resolution: 3.5 Å EMDB-49613: Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the Cysteine-Rich domains (CR) from the sucralose dataset Method: EM (single particle) / Resolution: 3.4 Å EMDB-49614: Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the sucralose dataset Method: EM (single particle) / Resolution: 4.3 Å EMDB-49615: Consensus map of the human sweet taste receptor (TAS1R2 + TAS1R3) from the sucralose dataset Method: EM (single particle) / Resolution: 3.7 Å 49616 9not EMDB-49616, PDB-9not: Human sweet taste receptor (TAS1R2 + TAS1R3) from the sucralose dataset Method: EM (single particle) / Resolution: 3.8 Å 49617 9nou EMDB-49617, PDB-9nou: Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains from the combined datasets Method: EM (single particle) / Resolution: 2.8 Å 49618 9nov EMDB-49618, PDB-9nov: Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains bound to sucralose Method: EM (single particle) / Resolution: 3.3 Å 49619 9now EMDB-49619, PDB-9now: Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains bound to aspartame Method: EM (single particle) / Resolution: 3.1 Å 49620 9nox EMDB-49620, PDB-9nox: Transmembrane domains of the human TAS1R2 sweet receptor subunit in complex with miniGs/gust25 Method: EM (single particle) / Resolution: 3.0 Å 49621 9noy EMDB-49621, PDB-9noy: Human TAS1R2 transmembrane domains from the TAS1R2-TM-G protein complex Method: EM (single particle) / Resolution: 3.7 Å 70072 9o38 EMDB-70072, PDB-9o38: Transmembrane domains of the human sweet receptor (TAS1R2 + TAS1R3) from Class 3 particles (rigidly fitted from PDB:9NOX and 9NOR) Method: EM (single particle) / Resolution: 3.0 Å EMDB-70073: Consensus map of the Human sweet taste receptor (TAS1R2 + TAS1R3) from the aspartame dataset Method: EM (single particle) / Resolution: 3.3 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
Source	homo sapiens (human) lama glama (llama)
Keywords	MEMBRANE PROTEIN / Sweet / taste / receptor / GPCR / TAS1R2 / TAS1R3 / T1R2 / T1R3 / human / HYDROLASE/IMMUNE SYSTEM / HYDROLASE-IMMUNE SYSTEM complex