[English] 日本語
Yorodumi
- EMDB-49612: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-49612
TitleHuman sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
Map dataHuman sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
Sample
  • Cell: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
    • Protein or peptide: Taste receptor type 1 member 3
    • Protein or peptide: Taste receptor type 1 member 2
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
KeywordsSweet / taste / receptor / GPCR / TAS1R2 / TAS1R3 / T1R2 / T1R3 / MEMBRANE PROTEIN
Function / homology
Function and homology information


sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste ...sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / receptor complex / G protein-coupled receptor signaling pathway / Golgi apparatus / membrane / plasma membrane
Similarity search - Function
GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR ...GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I
Similarity search - Domain/homology
Taste receptor type 1 member 3 / Taste receptor type 1 member 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsJuen Z / Lu Z / Yu R / Chang AN / Wang B / Fitzpatrick AWP / Zuker CS
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Cell / Year: 2025
Title: The structure of human sweetness.
Authors: Zhang Juen / Zhengyuan Lu / Ruihuan Yu / Andrew N Chang / Brian Wang / Anthony W P Fitzpatrick / Charles S Zuker /
Abstract: In humans, the detection and ultimately the perception of sweetness begin in the oral cavity, where taste receptor cells (TRCs) dedicated to sweet-sensing interact with sugars, artificial sweeteners, ...In humans, the detection and ultimately the perception of sweetness begin in the oral cavity, where taste receptor cells (TRCs) dedicated to sweet-sensing interact with sugars, artificial sweeteners, and other sweet-tasting chemicals. Human sweet TRCs express on their cell surface a sweet receptor that initiates the cascade of signaling events responsible for our strong attraction to sweet stimuli. Here, we describe the cryo-electron microscopy (cryo-EM) structure of the human sweet receptor bound to two of the most widely used artificial sweeteners-sucralose and aspartame. Our results reveal the structural basis for sweet detection, provide insights into how a single receptor mediates all our responses to such a wide range of sweet-tasting compounds, and open up unique possibilities for designing a generation of taste modulators informed by the structure of the human receptor.
History
DepositionMar 10, 2025-
Header (metadata) releaseMay 14, 2025-
Map releaseMay 14, 2025-
UpdateMay 21, 2025-
Current statusMay 21, 2025Processing site: RCSB / Status: Released

-
Structure visualization

Supplemental images

emd_49612.png

Downloads & links

-
Map

FileDownload / File: emd_49612.map.gz / Format: CCP4 / Size: 358.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHuman sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 456 pix.
= 375.288 Å		0.82 Å/pix.
x 453 pix.
= 372.819 Å		0.82 Å/pix.
x 455 pix.
= 374.465 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 0.823 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.22
Minimum - Maximum-0.21558103 - 1.2393076
Average (Standard dev.)0.00417696 (±0.018010179)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions453455456
Spacing455453456
CellA: 374.465 Å / B: 372.819 Å / C: 375.288 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Sample components

-
Entire : Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset

EntireName: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
Components
  • Cell: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
    • Protein or peptide: Taste receptor type 1 member 3
    • Protein or peptide: Taste receptor type 1 member 2
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

-
Supramolecule #1: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset

SupramoleculeName: Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Homo sapiens (human)

-
Macromolecule #1: Taste receptor type 1 member 3

MacromoleculeName: Taste receptor type 1 member 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 94.565969 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKTIIALSYI FCLVFADYKD DDDKAAAAPL CLSQQLRMKG DYVLGGLFPL GEAEEAGLRS RTRPSSPVCT RFSSNGLLWA LAMKMAVEE INNKSDLLPG LRLGYDLFDT CSEPVVAMKP SLMFLAKAGS RDIAAYCNYT QYQPRVLAVI GPHSSELAMV T GKFFSFFL ...String:
MKTIIALSYI FCLVFADYKD DDDKAAAAPL CLSQQLRMKG DYVLGGLFPL GEAEEAGLRS RTRPSSPVCT RFSSNGLLWA LAMKMAVEE INNKSDLLPG LRLGYDLFDT CSEPVVAMKP SLMFLAKAGS RDIAAYCNYT QYQPRVLAVI GPHSSELAMV T GKFFSFFL MPQVSYGASM ELLSARETFP SFFRTVPSDR VQLTAAAELL QEFGWNWVAA LGSDDEYGRQ GLSIFSALAA AR GICIAHE GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS EAWLTSDLVM GLP GMAQMG TVLGFLQRGA QLHEFPQYVK THLALATDPA FCSALGEREQ GLEEDVVGQR CPQCDCITLQ NVSAGLNHHQ TFSV YAAVY SVAQALHNTL QCNASGCPAQ DPVKPWQLLE NMYNLTFHVG GLPLRFDSSG NVDMEYDLKL WVWQGSVPRL HDVGR FNGS LRTERLKIRW HTSDNQKPVS RCSRQCQEGQ VRRVKGFHSC CYDCVDCEAG SYRQNPDDIA CTFCGQDEWS PERSTR CFR RRSRFLAWGE PAVLLLLLLL SLALGLVLAA LGLFVHHRDS PLVQASGGPL ACFGLVCLGL VCLSVLLFPG QPSPARC LA QQPLSHLPLT GCLSTLFLQA AEIFVESELP LSWADRLSGC LRGPWAWLVV LLAMLVEVAL CTWYLVAFPP EVVTDWHM L PTEALVHCRT RSWVSFGLAH ATNATLAFLC FLGTFLVRSQ PGRYNRARGL TFAMLAYFIT WVSFVPLLAN VQVVLRPAV QMGALLLCVL GILAAFHLPR CYLLMRQPGL NTPEFFLGGG PGDAQGQNDG NTGNQGKHE

UniProtKB: Taste receptor type 1 member 3

-
Macromolecule #2: Taste receptor type 1 member 2

MacromoleculeName: Taste receptor type 1 member 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 96.239008 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKTIIALSYI FCLVFAYPYD VPDYAAAAEP AENSDFYLPG DYLLGGLFSL HANMKGIVHL NFLQVPMCKE YEVKVIGYNL MQAMRFAVE EINNDSSLLP GVLLGYEIVD VCYISNNVQP VLYFLAHEDN LLPIQEDYSN YISRVVAVIG PDNSESVMTV A NFLSLFLL ...String:
MKTIIALSYI FCLVFAYPYD VPDYAAAAEP AENSDFYLPG DYLLGGLFSL HANMKGIVHL NFLQVPMCKE YEVKVIGYNL MQAMRFAVE EINNDSSLLP GVLLGYEIVD VCYISNNVQP VLYFLAHEDN LLPIQEDYSN YISRVVAVIG PDNSESVMTV A NFLSLFLL PQITYSAISD ELRDKVRFPA LLRTTPSADH HIEAMVQLML HFRWNWIIVL VSSDTYGRDN GQLLGERVAR RD ICIAFQE TLPTLQPNQN MTSEERQRLV TIVDKLQQST ARVVVVFSPD LTLYHFFNEV LRQNFTGAVW IASESWAIDP VLH NLTELR HLGTFLGITI QSVPIPGFSE FREWGPQAGP PPLSRTSQSY TCNQECDNCL NATLSFNTIL RLSGERVVYS VYSA VYAVA HALHSLLGCD KSTCTKRVVY PWQLLEEIWK VNFTLLDHQI FFDPQGDVAL HLEIVQWQWD RSQNPFQSVA SYYPL QRQL KNIQDISWHT INNTIPMSMC SKRCQSGQKK KPVGIHVCCF ECIDCLPGTF LNHTEDEYEC QACPNNEWSY QSETSC FKR QLVFLEWHEA PTIAVALLAA LGFLSTLAIL VIFWRHFQTP IVRSAGGPMC FLMLTLLLVA YMVVPVYVGP PKVSTCL CR QALFPLCFTI CISCIAVRSF QIVCAFKMAS RFPRAYSYWV RYQGPYVSMA FITVLKMVIV VIGMLATGLS PTTRTDPD D PKITIVSCNP NYRNSLLFNT SLDLLLSVVG FSFAYMGKEL PTNYNEAKFI TLSMTFYFTS SVSLCTFMSA YSGVLVTIV DLLVTVLNLL AISLGYFGPK CYMILFYPER NTPAYFNSMI QGYTMRRD

UniProtKB: Taste receptor type 1 member 2

-
Macromolecule #3: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 3 / Number of copies: 16 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 59.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 151281
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more