Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis.
Journal, issue, pages	Science, Vol. 388, Issue 6748, Page eadq7597, Year 2025
Publish date	May 15, 2025
Authors	Ruchao Peng / Xin Xu / Binod Nepal / Yikang Gong / Fenglin Li / Max B Ferretti / Mingyang Zhou / Kristen W Lynch / George M Burslem / Sandhya Kortagere / Ronen Marmorstein / Yi-Wei Chang /
PubMed Abstract	Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo-electron microscopy single-particle analysis and cryo- ...Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo-electron microscopy single-particle analysis and cryo-electron tomography, we define the influenza RNP as a right-handed, antiparallel double helix with the viral RNA encapsidated in the minor groove. Individual nucleoprotein subunits are connected by a flexible tail loop that inserts into a conserved pocket in its neighbor. We visualize the viral polymerase in RNP at different functional states, revealing how it accesses the RNA template while maintaining the double-helical architecture of RNP by strand sliding. Targeting the tail loop binding interface, we identify lead compounds as potential anti-influenza inhibitors. These findings elucidate the molecular determinants underpinning influenza virus replication and highlight a promising target for antiviral development.
External links	Science / PubMed:40373132 / PubMed Central
Methods	EM (helical sym.) / EM (single particle) / EM (subtomogram averaging)
Resolution	5.1 - 32.0 Å
Structure data	44980 9c4h EMDB-44980, PDB-9c4h: Double helical structure of influenza D RNP complex Method: EM (helical sym.) / Resolution: 8.6 Å 44981 9bwv EMDB-44981, PDB-9bwv: Structure of influenza D RNP, 4xNP local resconstruction Method: EM (single particle) / Resolution: 5.1 Å EMDB-44982: Loop end of influenza D virus RNP, class 1 Method: EM (single particle) / Resolution: 10.3 Å EMDB-44983: Structure of the loop end of influenza D virus RNP complex, class 2 Method: EM (single particle) / Resolution: 9.5 Å EMDB-44984: Structure of influenza A RNP, 4xNP local reconstruction, class 1 Method: EM (subtomogram averaging) / Resolution: 9.7 Å EMDB-44986: Structure of influenza A RNP, 4xNP local reconstruction, class 2 Method: EM (subtomogram averaging) / Resolution: 9.7 Å 44987 9bwz EMDB-44987, PDB-9bwz: Structure of influenza A RNP, 4xNP local reconstruction, class 3 Method: EM (subtomogram averaging) / Resolution: 9.2 Å 44989 9bx0 EMDB-44989, PDB-9bx0: Structure of influenza A RNP, 4xNP local reconstruction, class 4 Method: EM (subtomogram averaging) / Resolution: 9.2 Å 44990 9bx1 EMDB-44990, PDB-9bx1: Structure of influenza A RNP, 4xNP local reconstruction, class 5 Method: EM (subtomogram averaging) / Resolution: 8.0 Å 44993 9bx4 EMDB-44993, PDB-9bx4: Structure of influenza A RNP, 4xNP local reconstruction, class 6 Method: EM (subtomogram averaging) / Resolution: 9.2 Å EMDB-44994: NP subunits next to the polymerase at the end of influenza D virus RNP, piece 1 Method: EM (subtomogram averaging) / Resolution: 19.0 Å EMDB-44996: The influenza D virus polymerase at the end of RNP complex Method: EM (subtomogram averaging) / Resolution: 20.3 Å EMDB-44997: The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 1 Method: EM (subtomogram averaging) / Resolution: 17.0 Å EMDB-44998: The influenza D virus polymerase in the middle of RNP complex Method: EM (subtomogram averaging) / Resolution: 17.0 Å EMDB-45187: Subtomogram averaging of the polymerase end of influenza RNP complex Method: EM (subtomogram averaging) / Resolution: 32.0 Å EMDB-45188: Subtomogram averaging of influenza D virus RNP during elongation Method: EM (subtomogram averaging) / Resolution: 29.0 Å EMDB-48532: NP subunits next to the polymerase at the end of influenza D virus RNP, piece 2 Method: EM (subtomogram averaging) / Resolution: 19.0 Å EMDB-48533: The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 2 Method: EM (subtomogram averaging) / Resolution: 19.0 Å
Source	influenza d virus influenza a virus
Keywords	VIRAL PROTEIN/RNA / Influenza / Ribonucleoprotein complex / nucleoprotein / VIRAL PROTEIN-RNA complex / VIRAL PROTEIN / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex