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-Structure paper
| タイトル | Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription. |
|---|---|
| ジャーナル・号・ページ | Science, Vol. 387, Issue 6733, Page 528-533, Year 2025 |
| 掲載日 | 2025年1月31日 |
 著者 | Jonathan W Markert / Jelly H Soffers / Lucas Farnung |
| PubMed 要旨 | During transcription, RNA polymerase II traverses through chromatin, and posttranslational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine ...During transcription, RNA polymerase II traverses through chromatin, and posttranslational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine 36 trimethylation (H3K36me3), which is established by the histone methyltransferase SET domain containing 2 (SETD2), suppresses cryptic transcription, regulates splicing, and serves as a binding site for transcription elongation factors. The mechanism by which the transcription machinery coordinates the deposition of H3K36me3 is not well understood. Here we provide cryo-electron microscopy structures of mammalian RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome elongation complexes, revealing that the transcription machinery regulates H3K36me3 deposition by SETD2 on downstream and upstream nucleosomes. SPT6 binds the exposed H2A-H2B dimer during transcription, and the SPT6 death-like domain mediates an interaction with SETD2 bound to a nucleosome upstream of RNA polymerase II. |
 リンク | Science / PubMed:39666822 |
| 手法 | EM (単粒子) |
| 解像度 | 2.9 - 15.8 Å |
| 構造データ |  EMDB-47999: map A RNA Polymerase II  EMDB-48000: map B TFIIS  EMDB-48001: map C PAF1C  EMDB-48002: map D SPT6  EMDB-48003: map E IWS1  EMDB-48004: map F SETD2 nucleosome  EMDB-48005: map G hexasome  EMDB-48006: map H nucleosome  EMDB-48007: map I RNA Polymerase II  EMDB-48008: map J RNA Polymerase II +27 nucleosome  EMDB-48009: map K ~ -5 nucleosome  EMDB-48010: map L RNA Polymerase II  EMDB-48011: map M RNA Polymerase II intact nucleosome  EMDB-48012: map N secondary nucleosome  EMDB-48013: map O Partial Secondary nucleosome  EMDB-48014: map P Full secondary complex  EMDB-48015: map 1 RNA Polymerase II  EMDB-48016: map 2 PAF1C  EMDB-48017: map 3 SPT6  EMDB-48018: map4 IWS1 focused  EMDB-48019: map 5 SETD2 nucleosome  EMDB-48020: map6 SETD2 AID  EMDB-48021: map 7 SETD2-SPT6 nucleosome  EMDB-48022: map 8 Full upstream  EMDB-48023: map Alpha RNA Polymerase II  EMDB-48025: map gamma SPT6  EMDB-48026: map delta IWS1  EMDB-48027: map epsilon SETD2 nucleosome  EMDB-48028: map Zeta SETD2-SPT6 nucleosome  EMDB-48029: map Eta full upstream  EMDB-48030: map I FACT-RNA Polymerase II upstream complex  EMDB-48031: map II FACT upstream focused  EMDB-48032: map III SPT6 focused  EMDB-48033: map IV PAF1C  EMDB-48034: map V SETD2 nucleosome  EMDB-48035: map VI RNA Polymerase II  EMDB-48036: map VII Upstream SETD2  EMDB-48037: map VIII bp +115 upstream complex  EMDB-48038: map Q RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-hexasome, bp ~ -5 EMDB-48039, PDB-9egx: EMDB-48040, PDB-9egy: EMDB-48041, PDB-9egz: EMDB-48042, PDB-9eh0: EMDB-48043, PDB-9eh1: EMDB-48044, PDB-9eh2:  EMDB-48045: map X bp +115 upstream complex composite |
| 化合物 |  ChemComp-ZN:  ChemComp-MG: |
| 由来 |
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 キーワード | TRANSCRIPTION / TRANSFERASE/RNA/DNA / SETD2 / H3K36me3 / TRANSFERASE-RNA-DNA complex |
homo sapiens (ヒト)
sus scrofa (ブタ)
xenopus laevis (アフリカツメガエル)