EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of an archaeal ribosome reveals a divergent active site and hibernation factor.
ジャーナル・号・ページ	Nat Microbiol, Vol. 10, Issue 8, Page 1940-1953, Year 2025
掲載日	2025年7月17日
著者	Amos J Nissley / Yekaterina Shulgina / Roan W Kivimae / Blake E Downing / Petar I Penev / Jillian F Banfield / Dipti D Nayak / Jamie H D Cate /
PubMed 要旨	Ribosomes translate mRNA into protein. Despite divergence in ribosome structure over the course of evolution, the catalytic site, known as the peptidyl transferase centre (PTC), is thought to be ...Ribosomes translate mRNA into protein. Despite divergence in ribosome structure over the course of evolution, the catalytic site, known as the peptidyl transferase centre (PTC), is thought to be nearly universally conserved. Here we identify clades of archaea that have highly divergent ribosomal RNA sequences in the PTC. To understand how these PTC sequences fold, we determined cryo-EM structures of the 70S and 50S ribosomes to 2.4 Å and 2 Å, respectively, from the hyperthermophilic archaeon Pyrobaculum calidifontis. PTC sequence variation leads to the rearrangement of key base triples, and differences between archaeal and bacterial ribosomal proteins enable sequence variation in archaeal PTCs. Finally, we identify an archaeal ribosome hibernation factor, Dri, that differs from known bacterial and eukaryotic hibernation factors and is found in multiple archaeal phyla. Overall, this work identifies factors that regulate ribosome function in archaea and reveals a larger diversity of the most ancient sequences in the ribosome.
リンク	Nat Microbiol / PubMed:40676158
手法	EM (単粒子)
解像度	1.95 - 2.79 Å
構造データ	47578 9e6q EMDB-47578, PDB-9e6q: Cryo-EM structure of the Pyrobaculum calidifontis 50S ribosomal subunit in complex with Dri 手法: EM (単粒子) / 解像度: 1.95 Å EMDB-47604: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (Consensus Map) 手法: EM (単粒子) / 解像度: 2.36 Å EMDB-47605: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (50S focused refinement) 手法: EM (単粒子) / 解像度: 2.22 Å EMDB-47606: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (CP focused refinement) 手法: EM (単粒子) / 解像度: 2.42 Å EMDB-47611: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (30S focused refinement) 手法: EM (単粒子) / 解像度: 2.51 Å EMDB-47617: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (30S head focused refinement) 手法: EM (単粒子) / 解像度: 2.71 Å 47628 9e71 EMDB-47628: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (Composite Map) PDB-9e71: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome 手法: EM (単粒子) / 解像度: 2.36 Å EMDB-47662: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (Consensus Map) 手法: EM (単粒子) / 解像度: 2.53 Å EMDB-47664: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (50S focused refinement) 手法: EM (単粒子) / 解像度: 2.37 Å EMDB-47666: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (30S focused refinement) 手法: EM (単粒子) / 解像度: 2.67 Å EMDB-47667: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (30S head focused refinement) 手法: EM (単粒子) / 解像度: 2.79 Å 47668 9e7f EMDB-47668: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (Composite Map) PDB-9e7f: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri 手法: EM (単粒子) / 解像度: 2.53 Å
化合物	ChemComp-SPM: SPERMINE / スペルミン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
由来	pyrobaculum calidifontis jcm 11548 (古細菌)
キーワード	RIBOSOME / Translation / Hibernation / RNA