Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Multiple steps of dynein activation by Lis1 visualized by cryo-EM.
Journal, issue, pages	Nat Struct Mol Biol, Year 2025
Publish date	May 23, 2025
Authors	Agnieszka A Kendrick / Kendrick H V Nguyen / Wen Ma / Eva P Karasmanis / Rommie E Amaro / Samara L Reck-Peterson / Andres E Leschziner /
PubMed Abstract	Cytoplasmic dynein-1 (dynein) is an essential molecular motor controlled in part by autoinhibition. Lis1, a key dynein regulator mutated in the neurodevelopmental disease lissencephaly, plays a role ...Cytoplasmic dynein-1 (dynein) is an essential molecular motor controlled in part by autoinhibition. Lis1, a key dynein regulator mutated in the neurodevelopmental disease lissencephaly, plays a role in dynein activation. We recently identified a structure of partially autoinhibited dynein bound to Lis1, which suggests an intermediate state in dynein's activation pathway. However, other structural information is needed to fully understand how Lis1 activates dynein. Here, we used cryo-EM and yeast dynein and Lis1 incubated with ATP at different time points to reveal conformations that we propose represent additional intermediate states in dynein's activation pathway. We solved 16 high-resolution structures, including 7 distinct dynein and dynein-Lis1 structures from the same sample. Our data support a model in which Lis1 relieves dynein autoinhibition by increasing its basal ATP hydrolysis rate and promoting conformations compatible with complex assembly and motility. Together, this analysis advances our understanding of dynein activation and the contribution of Lis1 to this process.
External links	Nat Struct Mol Biol / PubMed:40410592
Methods	EM (single particle)
Resolution	2.8 - 4.1 Å
Structure data	46897 9di3 EMDB-46897, PDB-9di3: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 4.1 Å 46919 9diu EMDB-46919, PDB-9diu: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 4.1 Å 46935 9dj7 EMDB-46935, PDB-9dj7: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.8 Å 46938 9dju EMDB-46938, PDB-9dju: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 4.0 Å 46940 9djy EMDB-46940, PDB-9djy: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.6 Å 46941 9djz EMDB-46941, PDB-9djz: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.9 Å 46942 9dk0 EMDB-46942, PDB-9dk0: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.7 Å 46953 9dkd EMDB-46953, PDB-9dkd: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.3 Å 46954 9dke EMDB-46954, PDB-9dke: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.6 Å 46958 9dkh EMDB-46958, PDB-9dkh: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.9 Å 46959 9dkj EMDB-46959, PDB-9dkj: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 2.8 Å 46962 9dkm EMDB-46962, PDB-9dkm: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.4 Å 46972 9dkx EMDB-46972, PDB-9dkx: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.7 Å 46974 9dld EMDB-46974, PDB-9dld: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.2 Å 46975 9dle EMDB-46975, PDB-9dle: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.4 Å 47019 9dmw EMDB-47019, PDB-9dmw: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.7 Å 47026 9dn5 EMDB-47026, PDB-9dn5: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.3 Å 47032 9dn7 EMDB-47032, PDB-9dn7: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.25 Å 47033 9dnb EMDB-47033, PDB-9dnb: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. Method: EM (single particle) / Resolution: 3.0 Å
Chemicals	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry
Source	saccharomyces cerevisiae (brewer's yeast)
Keywords	MOTOR PROTEIN / Enzyme / AAA protein / AAA+ protein