Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural assembly of the tailed bacteriophage ϕ29.
Journal, issue, pages	Nat Commun, Vol. 10, Issue 1, Page 2366, Year 2019
Publish date	May 30, 2019
Authors	Jingwei Xu / Dianhong Wang / Miao Gui / Ye Xiang /
PubMed Abstract	The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile ...The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the ϕ29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-Å long, 24-strand β barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration.
External links	Nat Commun / PubMed:31147544 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.8 - 5.0 Å
Structure data	4655 6qvk EMDB-4655, PDB-6qvk: The cryo-EM structure of bacteriophage phi29 prohead Method: EM (single particle) / Resolution: 3.6 Å 4662 6qx7 EMDB-4662, PDB-6qx7: The cryo-EM structure of connector in bacteriophage phi29 prohead Method: EM (single particle) / Resolution: 3.8 Å 4677 6qyd EMDB-4677, PDB-6qyd: Cryo-EM structure of the head in mature bacteriophage phi29 Method: EM (single particle) / Resolution: 3.2 Å 4678 6qyj EMDB-4678, PDB-6qyj: The cryo-EM structure of the connector of the mature bacteriophage phi29 Method: EM (single particle) / Resolution: 3.4 Å 4679 6qym EMDB-4679, PDB-6qym: The cryo-EM structure of the connector of the genome empited bacteriophage phi29 Method: EM (single particle) / Resolution: 3.6 Å 4680 6qyz EMDB-4680, PDB-6qyz: The cryo-EM structure of prohead RNA in bacteriophage phi29 prohead Method: EM (single particle) / Resolution: 4.6 Å 4681 6qz0 EMDB-4681, PDB-6qz0: The cryo-EM structure of the head of the genome empited bacteriophage phi29 Method: EM (single particle) / Resolution: 3.2 Å EMDB-4682: The cryo-EM structure of the tail knob in the bacteriophage phi29 Method: EM (single particle) / Resolution: 5.0 Å EMDB-4683: The cryo-EM structure of the tail knob in genome empited bacteriophage phi29 Method: EM (single particle) / Resolution: 4.2 Å 4684 6qz9 EMDB-4684, PDB-6qz9: The cryo-EM structure of the collar complex and tail axis in bacteriophage phi29 Method: EM (single particle) / Resolution: 3.3 Å 4685 6qzf EMDB-4685, PDB-6qzf: The cryo-EM structure of the collar complex and tail axis in genome emptied bacteriophage phi29 Method: EM (single particle) / Resolution: 3.8 Å PDB-6qyy: The crystal structure of head fiber gp8.5 N base in bacteriophage phi29 Method: X-RAY DIFFRACTION / Resolution: 1.8 Å
Chemicals	ChemComp-SO4: SULFATE ION ChemComp-HOH: WATER
Source	bacillus phage phi29 (virus)
Keywords	VIRUS / bacteriophage / phi29 / prohead / cryo-EM / mature virus / genome emptied virion / mature virion