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Structure paper

TitleStructures of Native Doublet Microtubules from Trichomonas vaginalis Reveal Parasite-Specific Proteins.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 3996, Year 2025
Publish dateApr 29, 2025
AuthorsAlexander Stevens / Saarang Kashyap / Ethan H Crofut / Shuqi E Wang / Katherine A Muratore / Patricia J Johnson / Z Hong Zhou /
PubMed AbstractDoublet microtubules (DMTs) are flagellar components required for the protist Trichomonas vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the most common non- ...Doublet microtubules (DMTs) are flagellar components required for the protist Trichomonas vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the most common non-viral sexually transmitted disease. Lack of structures of Tv's DMT (Tv-DMT) has prevented structure-guided drug design to manage Tv infection. Here, we determine the 16 nm, 32 nm, 48 nm and 96 nm-repeat structures of native Tv-DMT at resolution ranging from 3.4 to 4.4 Å by cryogenic electron microscopy (cryoEM) and built an atomic model for the entire Tv-DMT. These structures show that Tv-DMT is composed of 30 different proteins, including the α- and β-tubulin, 19 microtubule inner proteins (MIPs) and 9 microtubule outer proteins. While the A-tubule of Tv-DMT is simplistic compared to DMTs of other organisms, the B-tubule of Tv-DMT features parasite-specific proteins, such as TvFAP40 and TvFAP35. Notably, TvFAP40 and TvFAP35 form filaments near the inner and outer junctions, respectively, and interface with stabilizing MIPs. This atomic model of the Tv-DMT highlights diversity of eukaryotic motility machineries and provides a structural framework to inform rational design of therapeutics against trichomoniasis.
External linksNat Commun / PubMed:40301421 / PubMed Central
MethodsEM (single particle)
Resolution3.19 - 4.7 Å
Structure data

EMDB-46009: Doublet Microtubules from T. Vaginalis - local refine 12
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-46020: Doublet Microtubule from T. vaginalis - local refine 25
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-46021: Doublet microtubules from T. vaginalis - local refine 26
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-46023: Doublet Microtubule from T. vaginalis - local refine 27
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-46024: Doublet microtubule from T. vaginalis - local refine 28
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-46026: Doublet microtubule from T. vaginalis - local refine 30
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-46028: Doublet microtubule from T. vaginalis - local refine 31
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-46029: Doublet Microtubules from T. vaginalis - local refine 32
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-46030: Doublet microtubule from T. vaginalis - local refine 33
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-46031: Doublet microtubule from T. vaginalis - local refine 24
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-46032: Doublet Microtubule from T. vaginalis - local refine 34
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-46033: Doublet Microtubule from T. vaginalis - local refine 35
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-46034: Doublet microtubules T. vaginalis local refine 20
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-46035: Doublet Microtubules T. vaginalis - local refine 21
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-46037: Doublet Microtubules from T. vaginalis - local refine 23
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-46038: Doublet microtubules from T. vaginalis - local refine 22
Method: EM (single particle) / Resolution: 3.75 Å

46580

9d5n

EMDB-46580, PDB-9d5n:
48-nm doublet microtubule from Trichomonas vaginalis strain G3
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-46633: Doublet Microtubules from T. vaginalis - local refine FAP40
Method: EM (single particle) / Resolution: 3.19 Å

EMDB-46634: Doublet Microtubules from T. vaginalis - local refine OJMOP1
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-46635: Doublet microtubules from T. vaginalis - 96nm NDRC local refine
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-46636: doublet microtubule from T. vaginalis - 96 nm repeat
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-46642: Doublet microtubule from T. vaginalis - 16nm
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-46643: Doublet Microtubules from T. vaginalis - 48 nm
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-48446: Radial spoke head from T. vaginalis
Method: EM (single particle) / Resolution: 4.7 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • trichomonas vaginalis g3 (eukaryote)
KeywordsSTRUCTURAL PROTEIN / doublet microtubule / tubulin / flagella

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