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TitleHigh-resolution reconstruction of a ribosome sheds light on evolutionary dynamics and tissue specificity.
Journal, issue, pagesRNA, Vol. 30, Issue 11, Page 1513-1528, Year 2024
Publish dateOct 16, 2024
AuthorsEnisha Sehgal / Chloe Wohlenberg / Evan M Soukup / Marcus J Viscardi / Vitor Hugo Balasco Serrão / Joshua A Arribere /
PubMed Abstract is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern ... is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern gene expression research is the presence of a high-resolution ribosome structure, though no such structure exists for Here, we present a high-resolution single-particle cryogenic electron microscopy (cryo-EM) reconstruction and molecular model of a ribosome, revealing a significantly streamlined animal ribosome. Many facets of ribosome structure are conserved in , including overall ribosomal architecture and the mechanism of cycloheximide, whereas other facets, such as expansion segments and eL28, are rapidly evolving. We identify uL5 and uL23 as two instances of tissue-specific ribosomal protein paralog expression conserved in , suggesting that ribosomes vary across tissues. The ribosome structure will provide a basis for future structural, biochemical, and genetic studies of translation in this important animal system.
External linksRNA / PubMed:39209556 / PubMed Central
MethodsEM (single particle)
Resolution2.59 - 2.63 Å
Structure data

44533

9bh5

EMDB-44533: High resolution C. elegans 80S ribosome structure
PDB-9bh5: High-resolution C. elegans 80S ribosome structure - class 1
Method: EM (single particle) / Resolution: 2.63 Å

45392

9cai

EMDB-45392, PDB-9cai:
High-resolution C. elegans 80S ribosome structure - class 1
Method: EM (single particle) / Resolution: 2.59 Å

Chemicals

ChemComp-SPD:
SPERMIDINE

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Source
  • caenorhabditis elegans (invertebrata)
KeywordsRIBOSOME / Caenorhabditis elegans / high-resolution single-particle analysis / endogenous purified 80S ribosome

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