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TitleDesign of customized coronavirus receptors.
Journal, issue, pagesNature, Vol. 635, Issue 8040, Page 978-986, Year 2024
Publish dateOct 30, 2024
AuthorsPeng Liu / Mei-Ling Huang / Hua Guo / Matthew McCallum / Jun-Yu Si / Yuan-Mei Chen / Chun-Li Wang / Xiao Yu / Lu-Lu Shi / Qing Xiong / Cheng-Bao Ma / John E Bowen / Fei Tong / Chen Liu / Ye-Hui Sun / Xiao Yang / Jing Chen / Ming Guo / Jing Li / Davide Corti / David Veesler / Zheng-Li Shi / Huan Yan /
PubMed AbstractAlthough coronaviruses use diverse receptors, the characterization of coronaviruses with unknown receptors has been impeded by a lack of infection models. Here we introduce a strategy to engineer ...Although coronaviruses use diverse receptors, the characterization of coronaviruses with unknown receptors has been impeded by a lack of infection models. Here we introduce a strategy to engineer functional customized viral receptors (CVRs). The modular design relies on building artificial receptor scaffolds comprising various modules and generating specific virus-binding domains. We identify key factors for CVRs to functionally mimic native receptors by facilitating spike proteolytic cleavage, membrane fusion, pseudovirus entry and propagation for various coronaviruses. We delineate functional SARS-CoV-2 spike receptor-binding sites for CVR design and reveal the mechanism of cell entry promoted by the N-terminal domain-targeting S2L20-CVR. We generated CVR-expressing cells for 12 representative coronaviruses from 6 subgenera, most of which lack known receptors, and show that a pan-sarbecovirus CVR supports propagation of a propagation-competent HKU3 pseudovirus and of authentic RsHuB2019A. Using an HKU5-specific CVR, we successfully rescued wild-type and ZsGreen-HiBiT-incorporated HKU5-1 (LMH03f) and isolated a HKU5 strain from bat samples. Our study demonstrates the potential of the CVR strategy for establishing native receptor-independent infection models, providing a tool for studying viruses that lack known susceptible target cells.
External linksNature / PubMed:39478224 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.2 Å
Structure data

45174

9c44

EMDB-45174, PDB-9c44:
SARS-CoV-2 S + S2L20
Method: EM (single particle) / Resolution: 2.6 Å

45175

9c45

EMDB-45175, PDB-9c45:
SARS-CoV-2 S + S2L20 (local refinement of NTD and S2L20 Fab variable region)
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / S2L20 / S / NTD / RBD / coronavirus / COVID-19 / SARS-CoV-2 / hexapro / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex

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