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-Structure paper
| タイトル | Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis. |
|---|---|
| ジャーナル・号・ページ | Science, Vol. 388, Issue 6748, Page eadq7597, Year 2025 |
| 掲載日 | 2025年5月15日 |
 著者 | Ruchao Peng / Xin Xu / Binod Nepal / Yikang Gong / Fenglin Li / Max B Ferretti / Mingyang Zhou / Kristen W Lynch / George M Burslem / Sandhya Kortagere / Ronen Marmorstein / Yi-Wei Chang /  |
| PubMed 要旨 | Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo-electron microscopy single-particle analysis and cryo- ...Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo-electron microscopy single-particle analysis and cryo-electron tomography, we define the influenza RNP as a right-handed, antiparallel double helix with the viral RNA encapsidated in the minor groove. Individual nucleoprotein subunits are connected by a flexible tail loop that inserts into a conserved pocket in its neighbor. We visualize the viral polymerase in RNP at different functional states, revealing how it accesses the RNA template while maintaining the double-helical architecture of RNP by strand sliding. Targeting the tail loop binding interface, we identify lead compounds as potential anti-influenza inhibitors. These findings elucidate the molecular determinants underpinning influenza virus replication and highlight a promising target for antiviral development. |
 リンク | Science / PubMed:40373132 / PubMed Central |
| 手法 | EM (らせん対称) / EM (単粒子) / EM (サブトモグラム平均) |
| 解像度 | 5.1 - 32.0 Å |
| 構造データ | EMDB-44980, PDB-9c4h: EMDB-44981, PDB-9bwv:  EMDB-44982: Loop end of influenza D virus RNP, class 1  EMDB-44983: Structure of the loop end of influenza D virus RNP complex, class 2  EMDB-44984: Structure of influenza A RNP, 4xNP local reconstruction, class 1  EMDB-44986: Structure of influenza A RNP, 4xNP local reconstruction, class 2 EMDB-44987, PDB-9bwz: EMDB-44989, PDB-9bx0: EMDB-44990, PDB-9bx1: EMDB-44993, PDB-9bx4:  EMDB-44994: NP subunits next to the polymerase at the end of influenza D virus RNP, piece 1  EMDB-44996: The influenza D virus polymerase at the end of RNP complex  EMDB-44997: The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 1  EMDB-44998: The influenza D virus polymerase in the middle of RNP complex  EMDB-45187: Subtomogram averaging of the polymerase end of influenza RNP complex  EMDB-45188: Subtomogram averaging of influenza D virus RNP during elongation  EMDB-48532: NP subunits next to the polymerase at the end of influenza D virus RNP, piece 2  EMDB-48533: The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 2 |
| 由来 |
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 キーワード | VIRAL PROTEIN/RNA / Influenza / Ribonucleoprotein complex / nucleoprotein / VIRAL PROTEIN-RNA complex / VIRAL PROTEIN / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex |
influenza d virus (インフルエンザウイルス)
influenza a virus (A型インフルエンザウイルス)