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TitleA surface lipoprotein on Pasteurella multocida binds complement factor I to promote immune evasion.
Journal, issue, pagesPLoS Pathog, Vol. 21, Issue 5, Page e1012686, Year 2025
Publish dateMay 6, 2025
AuthorsQuynh Huong Nguyen / Chun Heng Royce Lai / Michael J Norris / Dixon Ng / Megha Shah / Christine Chieh-Lin Lai / David E Isenman / Trevor F Moraes /
PubMed AbstractPasteurella multocida is the leading cause of wound infections in humans following animals' bites or scratches. This bacterium is also commonly found in the respiratory tract of many mammals and can ...Pasteurella multocida is the leading cause of wound infections in humans following animals' bites or scratches. This bacterium is also commonly found in the respiratory tract of many mammals and can cause serious diseases resulting in the rapid death of infected animals, especially cattle. To prevent these infections in cattle, a subunit-based vaccine utilizing the surface lipoprotein PmSLP was developed and showed remarkable protection with a single dose administration. Here, we report that PmSLP binds host complement factor I (FI) and facilitates cleavage of complement components C3b and C4b independently of any cofactors (e.g., FH, C4BP), thereby allowing the pathogen to evade host defence. Cryo-EM structure of PmSLP bound to FI reveals that PmSLP stimulates FI enzymatic activity by stabilizing the catalytic domain. This is the first time that a bacterial protein has been shown to directly activate FI independent of complement cofactors and target all arms of the complement cascade.
External linksPLoS Pathog / PubMed:40327719 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2 - 4.0 Å
Structure data

44139

9b3h

EMDB-44139, PDB-9b3h:
Structure of a complex between Pasteurella multocida surface lipoprotein, PmSLP-1, and bovine complement factor I
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-44146: Conformation 1 of the assembled dimer of PmSLP-1:FI complex
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-44149: Conformation 2 of the assembled dimer of PmSLP-1:FI complex
Method: EM (single particle) / Resolution: 3.4 Å

PDB-9b3e:
Crystal structure of a Slam-dependent surface lipoprotein, PmSLP, in Pasteurella multocida
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SE:
SELENIUM ATOM

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • bos taurus (domestic cattle)
  • pasteurella multocida 36950 (bacteria)
KeywordsMEMBRANE PROTEIN / Bacterial surface lipoprotein / complement evasion / complement protein / immune evasion

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