Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Visualizing late states of human 40S ribosomal subunit maturation.
Journal, issue, pages	Nature, Vol. 558, Issue 7709, Page 249-253, Year 2018
Publish date	Jun 6, 2018
Authors	Michael Ameismeier / Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed Abstract	The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high- ...The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.
External links	Nature / PubMed:29875412
Methods	EM (single particle)
Resolution	3.5 - 4.5 Å
Structure data	4337 6g18 EMDB-4337, PDB-6g18: Cryo-EM structure of a late human pre-40S ribosomal subunit - State C Method: EM (single particle) / Resolution: 3.6 Å 4348 6g4s EMDB-4348, PDB-6g4s: Cryo-EM structure of a late human pre-40S ribosomal subunit - State B Method: EM (single particle) / Resolution: 4.0 Å 4349 6g4w EMDB-4349, PDB-6g4w: Cryo-EM structure of a late human pre-40S ribosomal subunit - State A Method: EM (single particle) / Resolution: 4.5 Å 4350 6g51 EMDB-4350, PDB-6g51: Cryo-EM structure of a late human pre-40S ribosomal subunit - State D Method: EM (single particle) / Resolution: 4.1 Å 4351 6g53 EMDB-4351, PDB-6g53: Cryo-EM structure of a late human pre-40S ribosomal subunit - State E Method: EM (single particle) / Resolution: 4.5 Å 4352 6g5h EMDB-4352, PDB-6g5h: Cryo-EM structure of a late human pre-40S ribosomal subunit - Mature Method: EM (single particle) / Resolution: 3.6 Å 4353 6g5i EMDB-4353, PDB-6g5i: Cryo-EM structure of a late human pre-40S ribosomal subunit - State R Method: EM (single particle) / Resolution: 3.5 Å
Chemicals	ChemComp-ZN: Unknown entry
Source	homo sapiens (human)
Keywords	RIBOSOME / 40S / pre-40S / ribosome biogenesis